scholarly journals Biomolecules Responsible for the Total Antioxidant Capacity (TAC) of Human Plasma in Healthy and Cardiopathic Individuals: A Chemical Speciation Model

Antioxidants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 656
Author(s):  
Enrico Prenesti ◽  
Silvia Berto ◽  
Fabio Gosmaro ◽  
Marco Bagnati ◽  
Giorgio Bellomo
Keyword(s):  
Antioxidant Capacity ◽  
Human Plasma ◽  
Total Antioxidant Capacity ◽  
Chemical Speciation ◽  
Chemical Models ◽  
Speciation Model ◽  
Redox Active ◽  
Total Antioxidant ◽  
Exogenous Compounds ◽  
The Relationship

(1) Background: Much effort has been expended to investigate the antioxidant capacity of human plasma, attempting to clarify the roles of both metabolic and food substances in determining defenses against oxidative stress. The relationship between the total antioxidant capacity (TAC) and the concentrations of redox-active biomolecules in the human plasma of healthy and cardiopathic individuals was investigated in the present study to develop a chemical speciation model. (2) Methods: Plasma was collected from 85 blood donors and from 25 cardiovascular surgery patients. The TAC was measured using the CUPRAC-BCS (CUPric Reducing Antioxidant Capacity — Bathocuproinedisulfonic acid) method. Biomolecule concentrations were determined via visible spectrophotometry or HPLC/RP techniques. The relationship between the TAC and the concentrations was defined by applying a multiple regression analysis. The significance of the variables was first tested, and chemical models were proposed for the two datasets. The model equation is TAC=∑iβi∙[Ai] , where βi and [Ai] are the electronic exchange and the molar concentrations of the ith antioxidant component, respectively. (3) Results: The major contributions to the TAC, ~80%, come from endogenous compounds in both healthy and cardiopathic individuals, whereas the contributions from exogenous compounds were different between the two datasets. In particular, γ-tocopherol showed a different role in the chemical models developed for the two groups.

The association between Dietary total antioxidant capacity with Rheumatoid Arthritis in adults: A case-control study

2020 ◽  
Author(s):  
Shokufeh Nezamoleslami ◽  
Saeed Nezamoleslami ◽  
Reza Ghiasvand ◽  
Behnoosh Boozari ◽  
Mohammadhassan Sohouli
Keyword(s):  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Case Control Study ◽  
Case Control ◽  
Antioxidant Power ◽  
Vitamin B9 ◽  
Total Antioxidant ◽  
The Relationship ◽  
Control Study ◽  
Dietary Total Antioxidant Capacity

Abstract Objective Dietary total antioxidant capacity (TAC) has been assumed as a useful tool to assess the relationship between the cumulative antioxidant food capacity and several chronic disorders. However, the relationship between the total antioxidant capacity of the diet (TAC) and the risk of RA has not been previously examined. The aim of this study was to evaluate the association between DTAC and risk of RA in a case-control study. Methods This case-control study was carried out among 100 patients with RA and 200 healthy subjects aged 18–55 years. Dietary data were collected using a validated 147- items quantitative food frequency questionnaire. DTAC was calculated based on the ferric reducing-antioxidant power (FRAP) values that reported by US Department of Agriculture. Results The mean age and BMI of the study participants were 49.4 years and 25.6 Kg.m2.. The energy, protein, fiber, MUFA, n-3 fatty acid, vitamin B9, vegetable, and fruit were significantly increased across tertiles of DTAC. Also, Compared with participants in the lowest tertile of DTAC, those in the highest terrtile had a significantly lower OR for rheumatoid after adjustment for BMI; physical activity; waist circumference; smoking; and dietary intake energy and fat (model 3: OR, 0.19; 95% CI, 0.07–0.51; p for trend, 0.001). Conclusions Our findings showed that a high DTAC was associated with a reduced risk of RA in adult, suggesting that promoting a naturally elevated antioxidant capacity might help prevent the development of RA. Further prospective studies should be conducted in this regard.

scholarly journals Dysmetabolisms Can Affect Total Antioxidant Capacity (TAC) of Human Plasma: Determination of Reference Intervals of TAC by Way of CUPRAC-BCS Method

Antioxidants ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 58
Author(s):  
Enrico Prenesti ◽  
Silvia Berto ◽  
Fabio Gosmaro ◽  
Marco Bagnati ◽  
Giorgio Bellomo
Keyword(s):  
Antioxidant Capacity ◽  
Human Plasma ◽  
Total Antioxidant Capacity ◽  
Clinical Laboratory ◽  
Biological Fluid ◽  
Redox Status ◽  
Reference Intervals ◽  
Healthy Population ◽  
Pathological Conditions ◽  
Total Antioxidant

The total antioxidant capacity (TAC) of human plasma is an index of the redox buffer capacity of this biological fluid and could be a biomarker for those disorders affecting redox status. Distinguishing physiological from pathological conditions needs a reference. Therefore, this work aims to define the reference intervals for TAC of human plasma of apparently healthy adult individuals. TAC was measured using the CUPRAC-BCS (CUPric reducing antioxidant capacity-bathocuproinedisulfonic acid) method previously optimized and tested in a clinical laboratory. A population of 500 blood donors was selected, plus an additional 222 pathological patients carrying specific defective metabolisms, namely, hyperuricemia, hyperbilirubinemia, and type 2 diabetic mellitus. The reference intervals of TAC were calculated according to international guidelines. Due to the response of a partitioning test, the reference intervals for healthy population were separately defined for male (258) and female (151) groups. The reference intervals (µmol L−1) resulted: 727–1248 for the male subgroup and 637–1048 for the female subgroup. The absence of an age effect on TAC values was verified. The reference intervals evaluated allow a discussion on some pathological conditions overloading the plasma with redox-active waste substances.

scholarly journals A new automated method for the determination of the Total Antioxidant Capacity (TAC) of human plasma, based on the crocin bleaching assay

2002 ◽  
Vol 2 (1) ◽  
Author(s):  
Marilena Kampa ◽  
Anastasia Nistikaki ◽  
Vassilios Tsaousis ◽  
Niki Maliaraki ◽  
George Notas ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Human Plasma ◽  
Total Antioxidant Capacity ◽  
Automated Method ◽  
Total Antioxidant

scholarly journals Oxidative stress and total antioxidant capacity in human plasma

Redox Report ◽  
2009 ◽  
Vol 14 (3) ◽  
pp. 125-131 ◽  
Author(s):  
Alessandra Buico ◽  
Claudio Cassino ◽  
Mauro Ravera ◽  
Pier-Giacomo Betta ◽  
Domenico Osella
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Capacity ◽  
Human Plasma ◽  
Total Antioxidant Capacity ◽  
Total Antioxidant

scholarly journals The Relationship between Haptoglobin Polymorphism and Oxidative Stress in Hemodialysis Patients

2013 ◽  
Vol 32 (3) ◽  
pp. 220-226 ◽  
Author(s):  
Mawieh Hamad ◽  
Samir Awadallah ◽  
Hamzah Nasr
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Hemodialysis Patients ◽  
Haptoglobin Phenotype ◽  
Total Antioxidant ◽  
Ferroxidase Activity ◽  
The Relationship ◽  
And Oxidative Stress ◽  
Haptoglobin Polymorphism

Summary Background: The relationship between haptoglobin polymorphism and oxidative stress in hemodialysis patients is not fully understood. In this study, total antioxidant capacity and ce ru - loplasmin ferroxidase activity were evaluated in relation to haptoglobin phenotype distribution in hemodialysis patients. Methods: Serum samples collected from 161 patients and 84 healthy controls were haptoglobin-typed by electrophoresis. Ceruloplasmin ferroxidase activity and total antioxidant capacity were assayed using colorimetric methods. Results: Irrespective of the haptoglobin phenotype, patients exhibited significantly lower total antioxidant capacity (1.42± 0.29 vs. 1.55±0.28 mmol/L, P=0.002) and higher ferroxidase activity than controls. Frequency of Hp1-1 and Hp2-1 in patients was 15.5% and 36% as compared with 9.5% and 41.7% in controls. While ferroxidase activity was lower in Hp2-2 patients than in controls (142±61 vs. 179±47 U/L, P=0.002), it was higher in Hp2-1 (173±56 U/L) and Hp1-1 (170±54 U/L) patients than in controls (141±43 and 99±30 U/L respectively) (P=0.002 and 0.009). Ferroxidase activity in Hp2-2 patients was significantly lower than that of Hp2-1 or Hp1-1 patients (P=0.004 and 0.034). Total antioxidant capacity was significantly lower only in Hp2-2 patients (1.44±0.25) compared to that in Hp2-2 controls (1.65±0.22) (P=0.000). Conclusions:These findings suggest that haptoglobin polymorphism can differentially impact oxidative stress levels in hemodialysis patients.

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