biological fluid
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Micromachines ◽  
2021 ◽  
Vol 13 (1) ◽  
pp. 20
Author(s):  
Nikita Sitkov ◽  
Tatiana Zimina ◽  
Alexey Kolobov ◽  
Evgeny Sevostyanov ◽  
Valentina Trushlyakova ◽  
...  

A study of the peculiarities and a comparative analysis of the technologies used for the fabrication of elements of novel hybrid microfluidic biochips for express biomedical analysis have been carried out. The biochips were designed with an incorporated microfluidic system, which enabled an accumulation of the target compounds in a biological fluid to be achieved, thus increasing the biochip system’s sensitivity and even implementing a label-free design of the detection unit. The multilevel process of manufacturing a microfluidic system of a given topology for label-free fluorometric detection of protein structures is presented. The technological process included the chemical modification of the working surface of glass substrates by silanization using (3-aminopropyl) trimethoxysilane (APTMS), formation of the microchannels, for which SU-8 technologies and a last generation dry film photoresist were studied and compared. The solid-state phosphor layers were deposited using three methods: drop application; airbrushing; and mechanical spraying onto the adhesive surface. The processes of sealing the system, installing input ports, and packaging using micro-assembly technologies are described. The technological process has been optimized and the biochip was implemented and tested. The presented system can be used to design novel high-performance diagnostic tools that implement the function of express detection of protein markers of diseases and create low-power multimodal, highly intelligent portable analytical decision-making systems in medicine.


Materials ◽  
2021 ◽  
Vol 15 (1) ◽  
pp. 131
Author(s):  
Daniela Silva ◽  
Camila Arcos ◽  
Cecilia Montero ◽  
Carolina Guerra ◽  
Carola Martínez ◽  
...  

The increase in longevity worldwide has intensified the use of different types of prostheses for the human body, such as those used in dental work as well as in hip and knee replacements. Currently, Ti-6Al-4V is widely used as a joint implant due to its good mechanical properties and durability. However, studies have revealed that this alloy can release metal ions or particles harmful to human health. The mechanisms are not well understood yet and may involve wear and/or corrosion. Therefore, in this work, commercial pure titanium and a Ti-6Al-4V alloy were investigated before and after being exposed to a simulated biological fluid through tribological tests, surface analysis, and ionic dissolution characterization by ICP-AES. Before exposure, X-ray diffraction and optical microscopy revealed equiaxed α-Ti in both materials and β-Ti in Ti-6Al-4V. Scratch tests exhibited a lower coefficient of friction for Ti-6Al-4V alloy than commercially pure titanium. After exposure, X-ray photoelectron spectroscopy and surface-enhanced Raman spectroscopy results showed an oxide film formed by TiO2, both in commercially pure titanium and in Ti-6Al-4V, and by TiO and Al2O3 associated with the presence of the alloys. Furthermore, inductively coupled plasma atomic emission spectroscopy revealed that aluminum was the main ion released for Ti-6Al-4V, giving negligible values for the other metal ions.


Pramana ◽  
2021 ◽  
Vol 96 (1) ◽  
Author(s):  
Khurram Javid ◽  
Zeeshan Asghar ◽  
Umer Saeed ◽  
Muhammad Waqas

2021 ◽  
pp. 339350
Author(s):  
Michel Y. Fares ◽  
Nada S. Abdelwahab ◽  
Maha A. Hegazy ◽  
Maha M. Abdelrahman ◽  
Amr M. Mahmoud ◽  
...  

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yun-Jie Xu ◽  
Mubbashar Nazeer ◽  
Farooq Hussain ◽  
M. Ijaz Khan ◽  
M. K. Hameed ◽  
...  

AbstractThe multi-phase flow of non-Newtonian through a divergent channel is studied in this article. Jeffrey fluid is considered as the base liquid and tiny gold particles for the two-phase suspension. Application of external electric field parallel to complicated capillary with net surface charge density causes the bulk motion of the bi-phase fluid. In addition to, electro-osmotic flow with heat transfer, the simultaneous effects of viscous dissipation and nonlinear thermal radiation have also been incorporated. Finally, cumbersome mathematical manipulation yields a closed-form solution to the nonlinear differential equations. Parametric study reveals that more thermal energy is contributed in response to Brinkman number which significantly assists gold particles to more heat attain high temperature, as the remedy for compressed or swollen capillaries/arteries.


2021 ◽  
Author(s):  
Felix Kramer ◽  
Carl D Modes

A plethora of computational models have been developed in recent decades to account for the morphogenesis of complex biological fluid networks, such as capillary beds. Contemporary adaptation models are based on optimization schemes where networks react and adapt toward given flow patterns. Doing so, a system reduces dissipation and network volume, thereby altering its final form. Yet, recent numeric studies on network morphogenesis, incorporating uptake of metabolites by the embedding tissue, have indicated the conventional approach to be insufficient. Here, we systematically study a hybrid-model which combines the network adaptation schemes intended to generate space-filling perfusion as well as optimal filtration of metabolites. As a result, we find hydrodynamic stimuli (wall-shear stress) and filtration based stimuli (uptake of metabolites) to be antagonistic as hydrodynamically optimized systems have suboptimal uptake qualities and vice versa. We show that a switch between different optimization regimes is typically accompanied with a complex transition between topologically redundant meshes and spanning trees. Depending on the metabolite demand and uptake capabilities of the adaptating networks, we are further able to demonstrate the existence of nullity re-entrant behavior and the development of compromised phenotypes such as dangling non-perfused vessels and bottlenecks.


BMC Biology ◽  
2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Emilie Cardona ◽  
Cervin Guyomar ◽  
Thomas Desvignes ◽  
Jérôme Montfort ◽  
Samia Guendouz ◽  
...  

Abstract Background Circulating miRNAs (c-miRNAs) are found in most, if not all, biological fluids and are becoming well-established non-invasive biomarkers of many human pathologies. However, their features in non-pathological contexts and whether their expression profiles reflect normal life history events have received little attention, especially in non-mammalian species. The aim of the present study was to investigate the potential of c-miRNAs to serve as biomarkers of reproductive and metabolic states in fish. Results The blood plasma was sampled throughout the reproductive cycle of female rainbow trout subjected to two different feeding regimes that triggered contrasting metabolic states. In addition, ovarian fluid was sampled at ovulation, and all samples were subjected to small RNA-seq analysis, leading to the establishment of a comprehensive miRNA repertoire (i.e., miRNAome) and enabling subsequent comparative analyses to a panel of RNA-seq libraries from a wide variety of tissues and organs. We showed that biological fluid miRNAomes are complex and encompass a high proportion of the overall rainbow trout miRNAome. While sharing a high proportion of common miRNAs, the blood plasma and ovarian fluid miRNAomes exhibited strong fluid-specific signatures. We further revealed that the blood plasma miRNAome significantly changed depending on metabolic and reproductive states. We subsequently identified three evolutionarily conserved muscle-specific miRNAs or myomiRs (miR-1-1/2-3p, miR-133a-1/2-3p, and miR-206-3p) that accumulated in the blood plasma in response to high feeding rates, making these myomiRs strong candidate biomarkers of active myogenesis. We also identified miR-202-5p as a candidate biomarker for reproductive success that could be used to predict ovulation and/or egg quality. Conclusions Together, these promising results reveal the high potential of c-miRNAs, including evolutionarily conserved myomiRs, as physiologically relevant biomarker candidates and pave the way for the use of c-miRNAs for non-invasive phenotyping in various fish species.


2021 ◽  
Vol 6 (2) ◽  
pp. 136
Author(s):  
Rini Rahmiyati ◽  
Didit Aspriyanto ◽  
Beta Widya Oktiani

Background: Gingival crevicular fluid (GCF) is a biological fluid derived from the gingival sulcus and can be elevated in the inflammatory state of periodontal tissue, such as gingivitis. In previous studies, the number of GCF could also increase after panoramic radiographic exposure. Increase in GCF due to panoramic radiography is a sign of cell damage. Objective: To analyze the effects of panoramic radiography on the volume of GCF in wistar rats with gingivitis. Method: This type of research was true experimental with post test only and control group design. The sampling technique used was simple random sampling. A total of 25 wistar rats were classified into two control groups without exposure (state without gingivitis and gingivitis) and three groups treatment of exposure (state of gingivitis with 1 time, 2 times, and 3 times the exposure). GCF sampling using filter paper was carried out 10 minutes after panoramic radiographic exposure. The filter paper was stained by 2% ninhydrin solution, after that the GCF volume was calculated. Results: There was a significant difference in the number of GCF (p<0.05) in the group without gingivitis and exposure compared to all other groups, the gingivitis group without exposure compared to the gingivitis group with 3 times exposure, and the gingivitis group with 1 time exposure compared to the gingivitis group 3 times with exposure. Conclusion: Panoramic radiography can cause an increase in the volume of GCF in wistar rats with gingivitis. Keywords: GCF, gingivitis, panoramic radiography


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