scholarly journals Ethosomes for Coenzyme Q10 Cutaneous Administration: From Design to 3D Skin Tissue Evaluation

Antioxidants ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 485 ◽  
Author(s):  
Maddalena Sguizzato ◽  
Paolo Mariani ◽  
Francesco Spinozzi ◽  
Mascia Benedusi ◽  
Franco Cervellati ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Coenzyme Q10 ◽  
Ex Vivo ◽  
Human Epidermis ◽  
Transmission Electron ◽  
Endogenous Antioxidant ◽  
Cutaneous Administration ◽  
Ex Vivo Study ◽  
Cutaneous Application ◽  
3D Skin

Ethosome represents a smart transdermal vehicle suitable for solubilization and cutaneous application of drugs. Coenzyme Q10 is an endogenous antioxidant whose supplementation can counteract many cutaneous disorders and pathologies. In this respect, the present study describes the production, characterization, and cutaneous protection of phosphatidylcholine based ethosomes as percutaneous delivery systems for coenzyme Q10. CoQ10 entrapment capacity in ethosomes was almost 100%, vesicles showed the typical ‘fingerprint’ structure, while mean diameters were around 270 nm, undergoing an 8% increase after 3 months from production. An ex-vivo study, conducted by transmission electron microscopy, could detect the uptake of ethosomes in human skin fibroblasts and the passage of the vesicles through 3D reconstituted human epidermis. Immunofluorescence analyses were carried on both on fibroblasts and 3D reconstituted human epidermis treated with ethosomes in the presence of H2O2 as oxidative stress challenger, evaluating 4-hydroxynonenal protein adducts which is as a reliable biomarker for oxidative damage. Notably, the pretreatment with CoQ10 loaded in ethosomes exerted a consistent protective effect against oxidative stress, in both models, fibroblasts and in reconstituted human epidermis respectively.

scholarly journals Milk nutrition and childhood epilepsy: An ex vivo study on cytokines and oxidative stress in response to milk protein fractions

2018 ◽  
Vol 101 (6) ◽  
pp. 4842-4852 ◽  
Author(s):  
M. Albenzio ◽  
A. Santillo ◽  
M.G. Ciliberti ◽  
L. Figliola ◽  
M. Caroprese ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Milk Protein ◽  
Ex Vivo ◽  
Protein Fractions ◽  
Childhood Epilepsy ◽  
Ex Vivo Study ◽  
And Oxidative Stress

Assessment of principal parabens used in cosmetics after their passage through human epidermis?dermis layers (ex-vivo study)

2007 ◽  
Vol 16 (10) ◽  
pp. 830-836 ◽  
Author(s):  
Sawsan El Hussein ◽  
Patrice Muret ◽  
Michel Berard ◽  
Safwat Makki ◽  
Philippe Humbert
Keyword(s):  
Ex Vivo ◽  
Human Epidermis ◽  
Ex Vivo Study

Supplementation of ascorbic acid prevents oxidative damages in arsenic-loaded hepatic tissue of rat: An ex vivo study

2010 ◽  
Vol 29 (11) ◽  
pp. 965-972 ◽  
Author(s):  
Tanmoy Rana ◽  
Asit Kumar Bera ◽  
Subhashree Das ◽  
Diganta Pan ◽  
Subhasish Bandyopadhyay ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Ascorbic Acid ◽  
Ex Vivo ◽  
Protein Carbonyl ◽  
Hepatic Tissue ◽  
Arsenic Toxicity ◽  
Oxidative Damages ◽  
Hepatoprotective Agent ◽  
Ex Vivo Study ◽  
Screening Systems

Oxidative stress due to arsenic toxicity and ameliorative potentiality of L-ascorbic acid was evaluated in an ex vivo system of rat hepatic tissue. The study revealed that arsenic increased the activity of superoxide dismutase (SOD) and catalase (CAT) and the level of lipid peroxidation (LPO), protein carbonyl (PC) and nitric oxide (NO) at 1 hour, 1.5 hours and 2 hours of incubation. Co-treatment with L-ascorbic acid was found effective to normalize the activity of SOD and CAT and the production of LPO, PC and NO in hepatic tissue. This ex vivo study suggested that ascorbic acid is helpful to ameliorate arsenic-induced oxidative stress. This may be one of the alternative screening systems to study the efficacy of antioxidant and hepatoprotective agent.

The Role of Polyphenols in the Modulation of Plasma Non-Enzymatic Antioxidant Capacity (NEAC)

2012 ◽  
Vol 82 (3) ◽  
pp. 228-232 ◽  
Author(s):  
Mauro Serafini ◽  
Giuseppa Morabito
Keyword(s):  
Antioxidant Capacity ◽  
Dietary Intervention ◽  
Ex Vivo ◽  
The Body ◽  
Dietary Polyphenols ◽  
Enzymatic Antioxidant ◽  
Endogenous Antioxidant

Dietary polyphenols have been shown to scavenge free radicals, modulating cellular redox transcription factors in different in vitro and ex vivo models. Dietary intervention studies have shown that consumption of plant foods modulates plasma Non-Enzymatic Antioxidant Capacity (NEAC), a biomarker of the endogenous antioxidant network, in human subjects. However, the identification of the molecules responsible for this effect are yet to be obtained and evidences of an antioxidant in vivo action of polyphenols are conflicting. There is a clear discrepancy between polyphenols (PP) concentration in body fluids and the extent of increase of plasma NEAC. The low degree of absorption and the extensive metabolism of PP within the body have raised questions about their contribution to the endogenous antioxidant network. This work will discuss the role of polyphenols from galenic preparation, food extracts, and selected dietary sources as modulators of plasma NEAC in humans.

Direct Visualization of A2, A3, and A4 Finger Flexor Pulley Injuries at 3-T and 7-T MRI: An Ex Vivo Study

2020 ◽  
Author(s):  
Rafael Heiss ◽  
Frank W. Roemer ◽  
Christoph Lutter ◽  
Rolf Janka ◽  
Volker Schöffl ◽  
...  
Keyword(s):  
Ex Vivo ◽  
Direct Visualization ◽  
Ex Vivo Study

Impact of metal artefacts in QCT of the hip caused by a contralateral Gamma type nail: an ex vivo study

2014 ◽  
Author(s):  
Klaus Engelke ◽  
Nicolas Bouler ◽  
Oleg Museyko Fuerst ◽  
Sebastien Parratte ◽  
Thomas Fuerst ◽  
...  
Keyword(s):  
Ex Vivo ◽  
Metal Artefacts ◽  
Ex Vivo Study

Interactions between programmable shunt valves and magnetically controlled growing rods for scoliosis

2020 ◽  
Vol 26 (6) ◽  
pp. 667-670
Author(s):  
Thomas Larrew ◽  
Mohammed Alshareef ◽  
Robert F. Murphy ◽  
Ramin Eskandari ◽  
Libby Kosnik Infinger
Keyword(s):  
Remote Control ◽  
Ex Vivo ◽  
Shunt Valve ◽  
Growing Rods ◽  
Growing Rod ◽  
Before And After ◽  
Magnetically Controlled Growing Rods ◽  
Ex Vivo Study

OBJECTIVEAlthough the advent of magnetic growing rod technology for scoliosis has provided a means to bypass multiple hardware lengthening operations, it is important to be aware that many of these same patients have a codiagnosis of hydrocephalus with magnet-sensitive programmable ventricular shunts. As the magnetic distraction of scoliosis rods has not previously been described to affect the shunt valve setting, the authors conducted an investigation to characterize the interaction between the two devices.METHODSIn this ex vivo study, the authors carried out 360 encounters between four different shunt valve types at varying distances from the magnetic external remote control (ERC) used to distract the growing rods. Valve settings were examined before and after every interaction with the remote control to determine if there was a change in the setting.RESULTSThe Medtronic Strata and Codman Hakim valves were found to have setting changes at distances of 3 and 6 inches but not at 12 inches. The Aesculap proGAV and Codman Certas valves, typically described as MRI-resistant, did not have any setting changes due to the magnetic ERC regardless of distance.CONCLUSIONSAlthough it is not necessary to check a shunt valve after every magnetic distraction of scoliosis growing rods, if there is concern that the magnetic ERC may have been within 12 inches (30 cm) of a programmable ventricular shunt valve, the valve should be checked at the bedside with a programmer or with a skull radiograph along with postdistraction scoliosis radiographs.

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