Supplementation of ascorbic acid prevents oxidative damages in arsenic-loaded hepatic tissue of rat: An ex vivo study

2010 ◽  
Vol 29 (11) ◽  
pp. 965-972 ◽  
Author(s):  
Tanmoy Rana ◽  
Asit Kumar Bera ◽  
Subhashree Das ◽  
Diganta Pan ◽  
Subhasish Bandyopadhyay ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Ascorbic Acid ◽  
Ex Vivo ◽  
Protein Carbonyl ◽  
Hepatic Tissue ◽  
Arsenic Toxicity ◽  
Oxidative Damages ◽  
Hepatoprotective Agent ◽  
Ex Vivo Study ◽  
Screening Systems

Oxidative stress due to arsenic toxicity and ameliorative potentiality of L-ascorbic acid was evaluated in an ex vivo system of rat hepatic tissue. The study revealed that arsenic increased the activity of superoxide dismutase (SOD) and catalase (CAT) and the level of lipid peroxidation (LPO), protein carbonyl (PC) and nitric oxide (NO) at 1 hour, 1.5 hours and 2 hours of incubation. Co-treatment with L-ascorbic acid was found effective to normalize the activity of SOD and CAT and the production of LPO, PC and NO in hepatic tissue. This ex vivo study suggested that ascorbic acid is helpful to ameliorate arsenic-induced oxidative stress. This may be one of the alternative screening systems to study the efficacy of antioxidant and hepatoprotective agent.

scholarly journals Milk nutrition and childhood epilepsy: An ex vivo study on cytokines and oxidative stress in response to milk protein fractions

2018 ◽  
Vol 101 (6) ◽  
pp. 4842-4852 ◽  
Author(s):  
M. Albenzio ◽  
A. Santillo ◽  
M.G. Ciliberti ◽  
L. Figliola ◽  
M. Caroprese ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Milk Protein ◽  
Ex Vivo ◽  
Protein Fractions ◽  
Childhood Epilepsy ◽  
Ex Vivo Study ◽  
And Oxidative Stress

Oxidative stress in the testis of hyperglycemic rabbits treated with repaglinide

2007 ◽  
Vol 2 (4) ◽  
pp. 538-546
Author(s):  
Anna Gumieniczek ◽  
Hanna Hopkała ◽  
Marcin Pruchniak
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Ascorbic Acid ◽  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Protective Effect ◽  
Protein Carbonyl ◽  
Carbonyl Groups ◽  
Protein Carbonyl Groups ◽  
Diabetic Rabbits

AbstractIn the present study, the induction of oxidative stress was examined in the testis of alloxan-induced diabetic rabbits. In addition, the protective effect of repaglinide, an oral anti-diabetic, at a dose of 1 mg daily was studied after four and eight weeks of the treatment. For these purposes, the levels of superoxide dismutase (Cu,Zn-SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GSSG-R), glutathione (GSH), ascorbic acid (AA), lipid peroxidation products (LPO) and protein carbonyl groups (PCG) were quantified. Hyperglycemia resulted in significant increases in the antioxidative enzymes, Cu, Zn-SOD, CAT, GSH-Px, and GSSG-R after four and eight weeks, respectively. There was also an increase in GSH level, and a decrease in the level of AA. These effects were accompanied by an elevation in testicular LPO levels and PCG levels. Repaglinide was found to normalize the activity of GSSG-R and levels of GSH and AA, and blunted the increased lipid peroxidation, however no decrease in PCG levels were observed. In conclusion, some oxidative changes provoked in the testis of rabbits by hyperglycemia, were found to be reduced with repaglinide treatment at therapeutic dose.

scholarly journals Oxidative stress induced by the Fe2+/ascorbic acid system or model ischemia in vitro: effect of carvedilol and pyridoindole antioxidant SMe1EC2 in young and adult rat brain tissue

2010 ◽  
Vol 3 (4) ◽  
pp. 122-126 ◽  
Author(s):  
Zdenka Gáspárová ◽  
Oľga Ondrejičková ◽  
Alena Gajdošíková ◽  
Andrej Gajdošík ◽  
Vladimír Šnirc ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Ascorbic Acid ◽  
Rat Brain ◽  
Brain Tissue ◽  
Protein Carbonyl ◽  
Experimental Models ◽  
Adult Rat ◽  
Adult Rat Brain ◽  
Rat Brain Tissue

Oxidative stress induced by the Fe2+/ascorbic acid system or model ischemiain vitro: effect of carvedilol and pyridoindole antioxidant SMe1EC2 in young and adult rat brain tissueNew effective strategies and new highly effective neuroprotective agents are being searched for the therapy of human stroke and cerebral ischemia. The compound SMe1EC2 is a new derivative of stobadine, with enhanced antioxidant properties compared to the maternal drug. Carvedilol, a non-selective beta-blocker, possesses besides its cardioprotective and vasculoprotective properties also an antioxidant effect. We compared the effect of carvedilol and SMe1EC2, antioxidants with a similar chemical structure, in two experimental models of oxidative stress in young and adult rat brain tissue. SMe1EC2 was found to improve the resistance of hippocampal neurons to ischemiain vitroin young and even in 18-month-old rats and inhibited formation of protein carbonyl groups induced by the Fe2+/ascorbic acid pro-oxidative system in brain cortex homogenates of young rats. Carvedilol exerted a protective effect only in the hippocampus of 2-month-old rats and that at the concentration 10-times higher than did SMe1EC2. The inhibitory effect of carvedilol on protein carbonyl formation induced by the pro-oxidative system was not proved in the cortex of either young or adult rats. An increased baseline level of the content of protein carbonyl groups in the adult versus young rat brain cortex confirmed age-related changes in neuronal tissue and may be due to increased production of reactive oxygen species and low antioxidant defense mechanisms in the adult rat brain. The results revealed the new pyridoindole SMe1EC2 to be more effective than carvedilol in neuroprotection of rat brain tissue in both experimental models involving oxidative stress.

scholarly journals Ethosomes for Coenzyme Q10 Cutaneous Administration: From Design to 3D Skin Tissue Evaluation

Antioxidants ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 485 ◽  
Author(s):  
Maddalena Sguizzato ◽  
Paolo Mariani ◽  
Francesco Spinozzi ◽  
Mascia Benedusi ◽  
Franco Cervellati ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Coenzyme Q10 ◽  
Ex Vivo ◽  
Human Epidermis ◽  
Transmission Electron ◽  
Endogenous Antioxidant ◽  
Cutaneous Administration ◽  
Ex Vivo Study ◽  
Cutaneous Application ◽  
3D Skin

Ethosome represents a smart transdermal vehicle suitable for solubilization and cutaneous application of drugs. Coenzyme Q10 is an endogenous antioxidant whose supplementation can counteract many cutaneous disorders and pathologies. In this respect, the present study describes the production, characterization, and cutaneous protection of phosphatidylcholine based ethosomes as percutaneous delivery systems for coenzyme Q10. CoQ10 entrapment capacity in ethosomes was almost 100%, vesicles showed the typical ‘fingerprint’ structure, while mean diameters were around 270 nm, undergoing an 8% increase after 3 months from production. An ex-vivo study, conducted by transmission electron microscopy, could detect the uptake of ethosomes in human skin fibroblasts and the passage of the vesicles through 3D reconstituted human epidermis. Immunofluorescence analyses were carried on both on fibroblasts and 3D reconstituted human epidermis treated with ethosomes in the presence of H2O2 as oxidative stress challenger, evaluating 4-hydroxynonenal protein adducts which is as a reliable biomarker for oxidative damage. Notably, the pretreatment with CoQ10 loaded in ethosomes exerted a consistent protective effect against oxidative stress, in both models, fibroblasts and in reconstituted human epidermis respectively.

scholarly journals Arsenic trioxide and ascorbic acid interfere with the BCL2 family genes in patients with myelodysplastic syndromes: an ex-vivo study

2012 ◽  
Vol 5 (1) ◽  
Author(s):  
Sara Galimberti ◽  
Francesca Guerrini ◽  
Flavia Salvi ◽  
Iacopo Petrini ◽  
Daniela Gioia ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Arsenic Trioxide ◽  
Myelodysplastic Syndromes ◽  
Ex Vivo ◽  
Bcl2 Family ◽  
Ex Vivo Study

scholarly journals Effect of Alpha-lipoic Acid on the Metabolism of Arsenic in Arsenic-loaded Isolated Liver Tissues of Rat

2009 ◽  
Vol 12 (Number 1) ◽  
pp. 3-9
Author(s):  
N Tabassum
Keyword(s):  
Oxidative Stress ◽  
Ascorbic Acid ◽  
Lipoic Acid ◽  
Inorganic Arsenic ◽  
Arsenic Toxicity ◽  
Alpha Lipoic Acid ◽  
Arsenic Metabolism ◽  
Isolated Liver ◽  
Lipid Soluble Antioxidant ◽  
Liver Tissues

The patient of chronic arsenic toxicity shows oxidative stress. To overcome the oxidative stress. several antioxidants such as fl-carotene, ascorbic acid, attocopherol, sine and selenium had been suggested. in the present study. universal antioxidant (both water and lipid soluble antioxidant) alpha-lipoic acid was used to examine the effectiveness of this thin( on arsenic metabolism. Alpha-lipoic acid increases the inethylation of inorganic arsenic and releases nionomethylarsenic acid (MMA) and dimethylarstatic acid (DMA) from the haracellular pool. These results suggest that alpha-lipoic acid increases metabolism of arsenic thereby reduces the concentration of arsenic from arsenic-loaded isolated liver tissues of rat.

Direct Visualization of A2, A3, and A4 Finger Flexor Pulley Injuries at 3-T and 7-T MRI: An Ex Vivo Study

2020 ◽  
Author(s):  
Rafael Heiss ◽  
Frank W. Roemer ◽  
Christoph Lutter ◽  
Rolf Janka ◽  
Volker Schöffl ◽  
...  
Keyword(s):  
Ex Vivo ◽  
Direct Visualization ◽  
Ex Vivo Study
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