scholarly journals Structure of Microbial Communities When Complementary Effluents Are Anaerobically Digested

2021 ◽  
Vol 11 (3) ◽  
pp. 1293
Author(s):  
Ana Eusébio ◽  
André Neves ◽  
Isabel Paula Marques
Keyword(s):  
Anaerobic Digestion ◽  
Microbial Communities ◽  
Volatile Fatty Acids ◽  
Olive Mill Wastewater ◽  
Organic Load ◽  
Rrna Gene ◽  
Bacterial Populations ◽  
Next Generation Sequencing Ngs ◽  
Generation Sequencing ◽  
Olive Mill

Olive oil and pig productions are important industries in Portugal that generate large volumes of wastewater with high organic load and toxicity, raising environmental concerns. The principal objective of this study is to energetically valorize these organic effluents—piggery effluent and olive mill wastewater—through the anaerobic digestion to the biogas/methane production, by means of the effluent complementarity concept. Several mixtures of piggery effluent were tested, with an increasing percentage of olive mill wastewater. The best performance was obtained for samples of piggery effluent alone and in admixture with 30% of OMW, which provided the same volume of biogas (0.8 L, 70% CH4), 63/75% COD removal, and 434/489 L CH4/kg SVin, respectively. The validation of the process was assessed by molecular evaluation through Next Generation Sequencing (NGS) of the 16S rRNA gene. The structure of the microbial communities for both samples, throughout the anaerobic process, was characterized by the predominance of bacterial populations belonging to the phylum Firmicutes, mainly Clostridiales, with Bacteroidetes being the subdominant populations. Archaea populations belonging to the genus Methanosarcina became predominant throughout anaerobic digestion, confirming the formation of methane mainly from acetate, in line with the greatest removal of volatile fatty acids (VFAs) in these samples.

scholarly journals Limiting factors for anaerobic digestion of olive mill wastewater blends under mesophilic and thermophilic conditions

2018 ◽  
Vol 49 (2) ◽  
pp. 130-137
Author(s):  
Demetrio Antonio Zema ◽  
Giovanni Zappia ◽  
Souraya Benalia ◽  
Giuseppe Zimbalatti ◽  
Enzo Perri ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Anaerobic Digestion ◽  
Volatile Fatty Acids ◽  
Biogas Production ◽  
Olive Mill Wastewater ◽  
Limiting Factors ◽  
Citrus Peel ◽  
Thermophilic Conditions ◽  
High Concentrations ◽  
Olive Mill

Experimental trials of anaerobic digestion of olive mill wastewater (OMW) blended with other agro-industrial by-products were carried out to evaluate biogas production and sensitivity of the process to inhibiting compounds. Blends containing different percentages of OMW, digested liquid manure, and citrus peel were subjected to a batch anaerobic digestion process under both mesophilic and thermophilic conditions. The results showed that blends with percentages of OMW higher than 20% (v/v) had low methane yields due high concentrations of polyphenols (PPs) and/or volatile fatty acids (concentrations above 0.8 g kg–1 and 2.4 g L–1, respectively). The addition of other substrates such as citrus peel may have induced synergic inhibiting effects of PPs and essential oils (EO) on microbial growth. Thermophilic processes were more sensitive to these inhibiting compounds than mesophilic processes. The results of this study suggest that reducing PPs and EO concentrations in blends subject to anaerobic digestion below the inhibiting concentrations of 0.6 g L–1 and 0.5 g kg–1, respectively, is suitable. Additionally, it is advisable to maintain the volatile fatty acids content below 2 g L–1 to avoid its evident toxic effects on the growth of microorganisms in biochemical processes.

Response of methanogen populations to organic load increase during anaerobic digestion of olive mill wastewater

2006 ◽  
Vol 81 (9) ◽  
pp. 1556-1562 ◽  
Author(s):  
Aurora Rizzi ◽  
Mauro Zucchi ◽  
Sara Borin ◽  
Massimo Marzorati ◽  
Claudia Sorlini ◽  
...  
Keyword(s):  
Anaerobic Digestion ◽  
Olive Mill Wastewater ◽  
Organic Load ◽  
Load Increase ◽  
Olive Mill

Influence of hydrothermal pretreatment conditions, typology of anaerobic digestion system, and microbial profile in the production of volatile fatty acids from olive mill solid waste

2021 ◽  
Vol 9 (2) ◽  
pp. 105055
Author(s):  
Yasmim Arantes da Fonseca ◽  
Nayara Clarisse Soares Silva ◽  
Adonai Bruneli de Camargos ◽  
Silvana de Queiroz Silva ◽  
Hector Javier Luna Wandurraga ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Anaerobic Digestion ◽  
Solid Waste ◽  
Volatile Fatty Acids ◽  
Hydrothermal Pretreatment ◽  
Microbial Profile ◽  
Olive Mill Solid Waste ◽  
Pretreatment Conditions ◽  
Olive Mill

Enhancement of the anaerobic digestion of olive mill wastewater by the removal of phenolic inhibitors

1992 ◽  
Vol 27 (4) ◽  
pp. 231-237 ◽  
Author(s):  
R. Borja ◽  
A. Martin ◽  
R. Maestro ◽  
J. Alba ◽  
J.A. Fiestas
Keyword(s):  
Anaerobic Digestion ◽  
Olive Mill Wastewater ◽  
Olive Mill

scholarly journals Feasibility of Co-Treating Olive Mill Wastewater and Acid Mine Drainage

2020 ◽  
Vol 39 (4) ◽  
pp. 859-880
Author(s):  
Jorge Dias Carlier ◽  
Ana Teresa Luís ◽  
Luís Miguel Alexandre ◽  
Maria Clara Costa
Keyword(s):  
Acid Mine Drainage ◽  
Mine Drainage ◽  
Olive Mill Wastewater ◽  
Bacterial Populations ◽  
Limit Values ◽  
Acid Mine ◽  
Batch Tests ◽  
Successful Removal ◽  
Irrigation Waters ◽  
Olive Mill

Abstract Previous tests using a growth medium and olive mill wastewater (OMWW) have shown that it supplies carbon and electron donors suitable for sulphate reducing bacteria (SRB). We assessed the co-treatment of acid mine drainage (AMD) and OMWW using SRB-enriched bioreactors and identified the most abundant bacterial populations present under optimized conditions. The process requires a neutralizing agent to create optimal pH conditions for successful removal of the AMD’s main contaminants. Concentrations of SO42−, Al, Fe, Cu, Zn, and Mn decreased to below Portugal’s maximum admissible values for irrigation waters, and all but Mn were reduced to less than Portugal’s emission limit values (ELVs) for wastewater discharges. Phenol concentrations—the main pollutants in OMWW—dropped to values between 1/10 and 1/5 their initial concentrations in batch tests using mixtures of AMD and OMWW, and to 1/2 their initial concentrations in flow-through tests. The final total phenol concentrations were still above the ELV for wastewater discharges, but phenols are not regulated in irrigation waters, and OMWW is used by some producers to irrigate soils. Six main SRB groups were identified as likely having a fundamental role in the bioremediation process: the genera Desulfovibrio, Sulfurospirillum, and Acetobacter and the families Sphingomonadaceae, Prevotellaceae, and Deferribacteraceae.

scholarly journals Adaptation of Microbial Communities to Environmental Arsenic and Selection of Arsenite-Oxidizing Bacteria From Contaminated Groundwaters

2021 ◽  
Vol 12 ◽  
Author(s):  
Sarah Zecchin ◽  
Simona Crognale ◽  
Patrizia Zaccheo ◽  
Stefano Fazi ◽  
Stefano Amalfitano ◽  
...  
Keyword(s):  
Microbial Communities ◽  
Inorganic Arsenic ◽  
16S Rrna Gene Sequencing ◽  
Northern Italy ◽  
Rrna Gene ◽  
Bacterial Populations ◽  
Main Driver ◽  
Rrna Gene Sequencing ◽  
Arsenite Oxidizing Bacteria ◽  
Card Fish

Arsenic mobilization in groundwater systems is driven by a variety of functionally diverse microorganisms and complex interconnections between different physicochemical factors. In order to unravel this great ecosystem complexity, groundwaters with varying background concentrations and speciation of arsenic were considered in the Po Plain (Northern Italy), one of the most populated areas in Europe affected by metalloid contamination. High-throughput Illumina 16S rRNA gene sequencing, CARD-FISH and enrichment of arsenic-transforming consortia showed that among the analyzed groundwaters, diverse microbial communities were present, both in terms of diversity and functionality. Oxidized inorganic arsenic [arsenite, As(III)] was the main driver that shaped each community. Several uncharacterized members of the genus Pseudomonas, putatively involved in metalloid transformation, were revealed in situ in the most contaminated samples. With a cultivation approach, arsenic metabolisms potentially active at the site were evidenced. In chemolithoautotrophic conditions, As(III) oxidation rate linearly correlated to As(III) concentration measured at the parental sites, suggesting that local As(III) concentration was a relevant factor that selected for As(III)-oxidizing bacterial populations. In view of the exploitation of these As(III)-oxidizing consortia in biotechnology-based arsenic bioremediation actions, these results suggest that contaminated aquifers in Northern Italy host unexplored microbial populations that provide essential ecosystem services.

Kinetic study of anaerobic digestion of olive mill wastewater previously fermented with Aspergillus terreus

1993 ◽  
Vol 28 (6) ◽  
pp. 397-404 ◽  
Author(s):  
R. Borja ◽  
S.E. Garrido ◽  
L. Martínez ◽  
A. Ramos-Cormenzana ◽  
A. Martín
Keyword(s):  
Anaerobic Digestion ◽  
Kinetic Study ◽  
Aspergillus Terreus ◽  
Olive Mill Wastewater ◽  
Olive Mill

Performance and microbial analysis during long‐term anaerobic digestion of olive mill wastewater in a packed‐bed biofilm reactor

2019 ◽  
Vol 95 (3) ◽  
pp. 850-861 ◽  
Author(s):  
Ghizlane Enaime ◽  
Edith Nettmann ◽  
Stephan Berzio ◽  
Abdelaziz Baçaoui ◽  
Abdelrani Yaacoubi ◽  
...  
Keyword(s):  
Anaerobic Digestion ◽  
Packed Bed ◽  
Olive Mill Wastewater ◽  
Biofilm Reactor ◽  
Microbial Analysis ◽  
Olive Mill

scholarly journals Rapid Two Stage Anaerobic Digestion of Nejayote through Microaeration and Direct Interspecies Electron Transfer

Processes ◽  
2020 ◽  
Vol 8 (12) ◽  
pp. 1614
Author(s):  
David Valero ◽  
Carlos Rico ◽  
Raul Tapia-Tussell ◽  
Liliana Alzate-Gaviria
Keyword(s):  
Fatty Acids ◽  
Anaerobic Digestion ◽  
Volatile Fatty Acids ◽  
Uasb Reactor ◽  
Organic Load ◽  
Great Abundance ◽  
Conductive Material ◽  
Direct Interspecies Electron Transfer ◽  
Interspecies Electron Transfer ◽  
Granulated Activated Carbon

Corn is one of the main food products in Mexico. The elaboration of corn-derived products generates wastewater with a high organic load (nejayote). Anaerobic digestion is an indicated treatment for wastewater with high organic loads. The results of this study show that the application of microaeration in the hydrolysis-fermentative reactor increased the percentage of volatile fatty acids (VFA) available in the medium by 62%. The addition of a conductive material, such as granulated activated carbon (GAC), promotes DIET (Direct interspecies electrons transfer) in the methanogenic UASB reactor increasing the methane yield by 55%. Likewise, a great diversity of exoelectrogenic bacteria, with the ability to donate electrons DIET mechanisms, were developed in the GAC biofilm, though interestingly, Peptoclostridium and Clostridium (17.3% and 12.75%, respectively) were detected with a great abundance in the GAC biofilm. Peptoclostridium has not been previously reported as a participant in DIET process.

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