scholarly journals Perlecan in the Natural and Cell Therapy Repair of Human Adult Articular Cartilage: Can Modifications in This Proteoglycan Be a Novel Therapeutic Approach?

Biomolecules ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 92
Author(s):  
John Garcia ◽  
Helen S. McCarthy ◽  
Jan Herman Kuiper ◽  
James Melrose ◽  
Sally Roberts
Keyword(s):  
Articular Cartilage ◽  
Cell Therapy ◽  
Heparan Sulphate ◽  
Cartilage Defects ◽  
Human Articular Cartilage ◽  
Normal Cartilage ◽  
Embryonic Cartilage ◽  
Autologous Cell Therapy ◽  
Articular Cartilage Defects

Articular cartilage is considered to have limited regenerative capacity, which has led to the search for therapies to limit or halt the progression of its destruction. Perlecan, a multifunctional heparan sulphate (HS) proteoglycan, promotes embryonic cartilage development and stabilises the mature tissue. We investigated the immunolocalisation of perlecan and collagen between donor-matched biopsies of human articular cartilage defects (n = 10 × 2) that were repaired either naturally or using autologous cell therapy, and with age-matched normal cartilage. We explored how the removal of HS from perlecan affects human chondrocytes in vitro. Immunohistochemistry showed both a pericellular and diffuse matrix staining pattern for perlecan in both natural and cell therapy repaired cartilage, which related to whether the morphology of the newly formed tissue was hyaline cartilage or fibrocartilage. Immunostaining for perlecan was significantly greater in both these repair tissues compared to normal age-matched controls. The immunolocalisation of collagens type III and VI was also dependent on tissue morphology. Heparanase treatment of chondrocytes in vitro resulted in significantly increased proliferation, while the expression of key chondrogenic surface and genetic markers was unaffected. Perlecan was more prominent in chondrocyte clusters than in individual cells after heparanase treatment. Heparanase treatment could be a means of increasing chondrocyte responsiveness to cartilage injury and perhaps to improve repair of defects.

scholarly journals A composite scaffold of Wharton’s jelly and chondroitin sulphate loaded with human umbilical cord mesenchymal stem cells repairs articular cartilage defects in rat knee

2021 ◽  
Vol 32 (4) ◽  
Author(s):  
Zhong Li ◽  
Yikang Bi ◽  
Qi Wu ◽  
Chao Chen ◽  
Lu Zhou ◽  
...  
Keyword(s):  
Stem Cells ◽  
Articular Cartilage ◽  
Composite Scaffold ◽  
Biomechanical Properties ◽  
Cartilage Defects ◽  
Human Umbilical Cord ◽  
Composite Scaffolds ◽  
Articular Cartilage Defects

AbstractTo evaluate the performance of a composite scaffold of Wharton’s jelly (WJ) and chondroitin sulfate (CS) and the effect of the composite scaffold loaded with human umbilical cord mesenchymal stem cells (hUCMSCs) in repairing articular cartilage defects, two experiments were carried out. The in vitro experiments involved identification of the hUCMSCs, construction of the biomimetic composite scaffolds by the physical and chemical crosslinking of WJ and CS, and testing of the biomechanical properties of both the composite scaffold and the WJ scaffold. In the in vivo experiments, composite scaffolds loaded with hUCMSCs and WJ scaffolds loaded with hUCMSCs were applied to repair articular cartilage defects in the rat knee. Moreover, their repair effects were evaluated by the unaided eye, histological observations, and the immunogenicity of scaffolds and hUCMSCs. We found that in vitro, the Young’s modulus of the composite scaffold (WJ-CS) was higher than that of the WJ scaffold. In vivo, the composite scaffold loaded with hUCMSCs repaired rat cartilage defects better than did the WJ scaffold loaded with hUCMSCs. Both the scaffold and hUCMSCs showed low immunogenicity. These results demonstrate that the in vitro construction of a human-derived WJ-CS composite scaffold enhances the biomechanical properties of WJ and that the repair of knee cartilage defects in rats is better with the composite scaffold than with the single WJ scaffold if the scaffold is loaded with hUCMSCs.

Histological and biochemical evaluation of perichondrial transplants in human articular cartilage defects

1999 ◽  
Vol 17 (6) ◽  
pp. 843-849 ◽  
Author(s):  
Phianne Bouwmeester ◽  
Roel Kuijer ◽  
Els Terwindt-Rouwenhorst ◽  
Ton van der Linden ◽  
Sjoerd Bulstra
Keyword(s):  
Articular Cartilage ◽  
Cartilage Defects ◽  
Human Articular Cartilage ◽  
Biochemical Evaluation ◽  
Articular Cartilage Defects

Study on Polyvinyl Alcohol Hydrogel Materials for Improving the Performance of Artificial Articular Cartilage

2013 ◽  
Vol 703 ◽  
pp. 29-32 ◽  
Author(s):  
Hou Jiang Yuan ◽  
Zhou Jian Wei ◽  
Xia Zhen Yu
Keyword(s):  
Articular Cartilage ◽  
Polyvinyl Alcohol ◽  
Impact Force ◽  
Biomechanical Properties ◽  
Research Progress ◽  
Cartilage Defects ◽  
Human Articular Cartilage ◽  
The Impact ◽  
Articular Cartilage Defects

Polyvinyl alcohol hydrogel has compatibility and biomechanical properties of human articular cartilage similar and good biological. The implantation in the human body can replace part of articular cartilage, which plays the role of bearing and alleviate the impact force. It has the prospect of clinical application. This paper introduces the research progress of polyvinyl alcohol hydro-gel materials. And compared with the characteristics of articular cartilage, clarify the possibility of repair of articular cartilage defects of the materials.

scholarly journals Silk fibroin hydrogel scaffolds incorporated with chitosan nanoparticles repair articular cartilage defects by regulating TGF-β1 and BMP-2

2021 ◽  
Vol 23 (1) ◽  
Author(s):  
Yuan Li ◽  
Yanping Liu ◽  
Qiang Guo
Keyword(s):  
Articular Cartilage ◽  
Knee Joint ◽  
Silk Fibroin ◽  
Cartilage Defects ◽  
Joint Cartilage ◽  
Hydrogel Scaffolds ◽  
Articular Cartilage Defects ◽  
Tgf Β1

AbstractCartilage defects frequently occur around the knee joint yet cartilage has limited self-repair abilities. Hydrogel scaffolds have excellent potential for use in tissue engineering. Therefore, the aim of the present study was to assess the ability of silk fibroin (SF) hydrogel scaffolds incorporated with chitosan (CS) nanoparticles (NPs) to repair knee joint cartilage defects. In the present study, composite systems of CS NPs incorporated with transforming growth factor-β1 (TGF-β1; TGF-β1@CS) and SF incorporated with bone morphogenetic protein-2 (BMP-2; TGF-β1@CS/BMP-2@SF) were developed and characterized with respect to their size distribution, zeta potential, morphology, and release of TGF-β1 and BMP-2. Bone marrow stromal cells (BMSCs) were co-cultured with TGF-β1@CS/BMP-2@SF extracts to assess chondrogenesis in vitro using a cell counting kit-8 assay, which was followed by in vivo evaluations in a rabbit model of knee joint cartilage defects. The constructed TGF-β1@CS/BMP-2@SF composite system was successfully characterized and showed favorable biocompatibility. In the presence of TGF-β1@CS/BMP-2@SF extracts, BMSCs exhibited normal cell morphology and enhanced chondrogenic ability both in vitro and in vivo, as evidenced by the promotion of cell viability and the alleviation of cartilage defects. Thus, the TGF-β1@CS/BMP-2@SF hydrogel developed in the present study promoted chondrogenic ability of BMSCs both in vivo and in vitro by releasing TGF-β1 and BMP-2, thereby offering a novel therapeutic strategy for repairing articular cartilage defects in knee joints.

scholarly journals Repair of full-thickness articular cartilage defects using IEIK13 self-assembling peptide hydrogel in a non-human primate model

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Alexandre Dufour ◽  
Jérôme E. Lafont ◽  
Marie Buffier ◽  
Michaël Verset ◽  
Angéline Cohendet ◽  
...  
Keyword(s):  
Articular Cartilage ◽  
Articular Chondrocytes ◽  
Cartilage Tissue ◽  
Cartilage Defects ◽  
Full Thickness ◽  
Primate Model ◽  
Self Assembling ◽  
Articular Cartilage Defects ◽  
Human Primate

AbstractArticular cartilage is built by chondrocytes which become less active with age. This declining function of the chondrocytes, together with the avascular nature of the cartilage, impedes the spontaneous healing of chondral injuries. These lesions can progress to more serious degenerative articular conditions as in the case of osteoarthritis. As no efficient cure for cartilage lesions exist yet, cartilage tissue engineering has emerged as a promising method aiming at repairing joint defects and restoring articular function. In the present work, we investigated if a new self-assembling peptide (referred as IEIK13), combined with articular chondrocytes treated with a chondrogenic cocktail (BMP-2, insulin and T3, designated BIT) could be efficient to restore full-thickness cartilage defects induced in the femoral condyles of a non-human primate model, the cynomolgus monkey. First, in vitro molecular studies indicated that IEIK13 was efficient to support production of cartilage by monkey articular chondrocytes treated with BIT. In vivo, cartilage implant integration was monitored non-invasively by contrast-enhanced micro-computed tomography, and then by post-mortem histological analysis and immunohistochemical staining of the condyles collected 3 months post-implantation. Our results revealed that the full-thickness cartilage injuries treated with either IEIK13 implants loaded with or devoid of chondrocytes showed similar cartilage-characteristic regeneration. This pilot study demonstrates that IEIK13 can be used as a valuable scaffold to support the in vitro activity of articular chondrocytes and the repair of articular cartilage defects, when implanted alone or with chondrocytes.

Histological and Biochemical Evaluation of Perichondrial Transplants in Human Articular Cartilage Defects

2000 ◽  
Vol 82 (4) ◽  
pp. 48
Author(s):  
Phianne Bouwmeester ◽  
Roel Kuijer ◽  
Els Terwindt-Rouwenhorst ◽  
Ton van der Linden ◽  
Sjoerd Bulstra
Keyword(s):  
Articular Cartilage ◽  
Cartilage Defects ◽  
Human Articular Cartilage ◽  
Biochemical Evaluation ◽  
Articular Cartilage Defects
Sign in / Sign up

Export Citation Format

Share Document