New Insights to Regulation of Fructose-1,6-bisphosphatase during Anoxia in Red-Eared Slider, Trachemys scripta elegans

The red-eared slider (Trachemys scripta elegans) undergoes numerous changes to its physiological and metabolic processes to survive without oxygen. During anoxic conditions, its metabolic rate drops drastically to minimize energy requirements. The alterations in the central metabolic pathways are often accomplished by the regulation of key enzymes. The regulation of one such enzyme, fructose-1,6-bisphosphatase (FBPase; EC 3.1.3.11), was characterized in the present study during anoxia in liver. FBPase is a crucial enzyme of gluconeogenesis. The FBPase was purified from liver tissue in both control and anoxic conditions and subsequently assayed to determine the kinetic parameters of the enzyme. The study revealed the relative degree of post-translational modifications in the FBPase from control and anoxic turtles. Further, this study demonstrated a significant decrease in the maximal activity in anoxic FBPase and decreased sensitivity to its substrate fructose-1,6-bisphosphate (FBP) when compared to the control. Immunoblotting demonstrated increased threonine phosphorylation (~1.4-fold) in the anoxic FBPase. Taken together, these results suggest that the phosphorylation of liver FBPase is an important step in suppressing FBPase activity, ultimately leading to the inhibition of gluconeogenesis in the liver of the red-eared slider during anaerobic conditions.

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Characterization of Fructose-1,6-Bisphosphate Aldolase during Anoxia in the Tolerant Turtle, Trachemys scripta elegans: An Assessment of Enzyme Activity, Expression and Structure

PLoS ONE ◽

10.1371/journal.pone.0068830 ◽

2013 ◽

Vol 8 (7) ◽

pp. e68830 ◽

Cited By ~ 11

Author(s):

Neal J. Dawson ◽

Kyle K. Biggar ◽

Kenneth B. Storey

Keyword(s):

Enzyme Activity ◽

Trachemys Scripta ◽

Trachemys Scripta Elegans ◽

Fructose 1,6 Bisphosphate

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INABILITY TO INDUCE TYMPANIC SQUAMOUS METAPLASIA USING ORGANOCHLORINE COMPOUNDS IN VITAMIN A-DEFICIENT RED-EARED SLIDERS (TRACHEMYS SCRIPTA ELEGANS)

Journal of Wildlife Diseases ◽

10.7589/0090-3558-44.3.664 ◽

2008 ◽

Vol 44 (3) ◽

pp. 664-669 ◽

Cited By ~ 5

Author(s):

Karl R. Kroenlein ◽

Jonathan M. Sleeman ◽

Steven D. Holladay ◽

Priscilla H. Joyner ◽

Justin D. Brown ◽

...

Keyword(s):

Vitamin A ◽

Squamous Metaplasia ◽

Trachemys Scripta ◽

Organochlorine Compounds ◽

Trachemys Scripta Elegans

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Helminths from the red-eared slider Trachemys scripta elegans (Chelonia: Emydidae) in marshes from the eastern Iberian Peninsula: first report of Telorchis attenuata (Digenea: Telorchiidae)

Basic and Applied Herpetology ◽

10.11160/bah.38 ◽

2014 ◽

Vol 28 ◽

Author(s):

JesÃºs Cardells ◽

MarÃa Magdalena Garijo ◽

Clara MarÃn ◽

Santiago Vera

Keyword(s):

Iberian Peninsula ◽

Intestinal Tract ◽

Trachemys Scripta ◽

Trachemys Scripta Elegans ◽

Emys Orbicularis ◽

First Report ◽

Digenean Trematode ◽

Limited Sampling ◽

Mauremys Leprosa ◽

New Habitats

The present work describes the presence of a digenean in the red-eared turtle Trachemys scripta elegans (Wied-Neuwied, 1839) in marshes of the Valencian Community. The faeces and intestinal tract of 105 animals were examined. Only one helminth species was found and identified as the digenean trematode Telorchis atenuatta (Goldberger, 1911), present in the 7.6% of the animals analysed. This is the first report of the parasite in sliders from Spain. Although conclusions are preliminary due to the limited sampling, our results suggest that the presence of red-eared turtles in new habitats may increase the risk of introducing new microorganisms and new diseases with them, altering the sanitary status of the autochthonous terrapins Mauremys leprosa (Schweigger, 1812) and Emys orbicularis (Linnaeus, 1758).

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Metabolism-driven post-translational modifications of H3K9 in early bovine embryos

Reproduction ◽

10.1530/rep-21-0134 ◽

2021 ◽

Vol 162 (3) ◽

pp. 181-191

Author(s):

Jessica Ispada ◽

Aldcejam Martins da Fonseca Junior ◽

Otávio Luiz Ramos Santos ◽

Camila Bruna de Lima ◽

Erika Cristina dos Santos ◽

...

Keyword(s):

Metabolic Pathways ◽

Developmental Stages ◽

De Novo ◽

Blastocyst Stage ◽

Developmental Model ◽

Bovine Embryos ◽

Acetyl Coa ◽

Post Translational Modifications ◽

The Relationship ◽

Different Levels

Metabolic and molecular profiles were reported as different for bovine embryos with distinct kinetics during the first cleavages. In this study, we used this same developmental model (fast vs slow) to determine if the relationship between metabolism and developmental kinetics affects the levels of acetylation or tri-methylation at histone H3 lysine 9 (H3K9ac and H3K9me3, respectively). Fast and slow developing embryos presented different levels of H3K9ac and H3K9me3 from the earliest stages of development (40 and 96 hpi) and up to the blastocyst stage. For H3K9me3, both groups of embryos presented a wave of demethylation and de novo methylation, although it was more pronounced in fast than slow embryos, resulting in blastocysts with higher levels of this mark. The H3K9ac reprogramming profile was distinct between kinetics groups. While slow embryos presented a wave of deacetylation, followed by an increase in this mark at the blastocyst stage, fast embryos reduced this mark throughout all the developmental stages studied. H3K9me3 differences corresponded to writer and eraser transcript levels, while H3K9ac patterns were explained by metabolism-related gene expression. To verify if metabolic differences could alter levels of H3K9ac, embryos were cultured with sodium-iodoacetate (IA) or dichloroacetate (DCA) to disrupt the glycolytic pathway or increase acetyl-CoA production, respectively. IA reduced H3K9ac while DCA increased H3K9ac in blastocysts. Concluding, H3K9me3 and H3K9ac patterns differ between embryos with different kinetics, the second one explained by metabolic pathways involved in acetyl-CoA production. So far, this is the first study demonstrating a relationship between metabolic differences and histone post-translational modifications in bovine embryos.

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