scholarly journals The Antibacterial Activity of Human Amniotic Membrane against Multidrug-Resistant Bacteria Associated with Urinary Tract Infections: New Insights from Normal and Cancerous Urothelial Models

Biomedicines ◽  
2021 ◽  
Vol 9 (2) ◽  
pp. 218
Author(s):  
Taja Železnik Ramuta ◽  
Larisa Tratnjek ◽  
Aleksandar Janev ◽  
Katja Seme ◽  
Marjanca Starčič Erjavec ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Amniotic Membrane ◽  
Multidrug Resistant ◽  
Basic Knowledge ◽  
Resistant Bacteria ◽  
Human Amniotic Membrane ◽  
Multidrug Resistant Bacteria ◽  
Tract Infections

Urinary tract infections (UTIs) represent a serious global health issue, especially due to emerging multidrug-resistant UTI-causing bacteria. Recently, we showed that the human amniotic membrane (hAM) could be a candidate for treatments and prevention of UPEC and Staphylococcus aureus infections. However, its role against multidrug-resistant bacteria, namely methicillin-resistant S. aureus (MRSA), extended-spectrum beta-lactamases (ESBL) producing Escherichia coli and Klebsiella pneumoniae, vancomycin-resistant Enterococci (VRE), carbapenem-resistant Acinetobacter baumannii, and Pseudomonas aeruginosa has not yet been thoroughly explored. Here, we demonstrate for the first time that the hAM homogenate had antibacterial activity against 7 out of 11 tested multidrug-resistant strains, the greatest effect was on MRSA. Using novel approaches, its activity against MRSA was further evaluated in a complex microenvironment of normal and cancerous urinary bladder urothelia. Even short-term incubation in hAM homogenate significantly decreased the number of bacteria in MRSA-infected urothelial models, while it did not affect the viability, number, and ultrastructure of urothelial cells. The hAM patches had no antibacterial activity against any of the tested strains, which further exposes the importance of the hAM preparation. Our study substantially contributes to basic knowledge on the antibacterial activity of hAM and reveals its potential to be used as an antibacterial agent against multidrug-resistant bacteria.

Intravesical Gentamicin Treatment for Recurrent Urinary Tract Infections Caused by Multidrug Resistant Bacteria

2019 ◽  
Vol 201 (3) ◽  
pp. 549-555 ◽  
Author(s):  
Janneke E. Stalenhoef ◽  
Cees van Nieuwkoop ◽  
Petra H. Menken ◽  
Sandra T. Bernards ◽  
Henk W. Elzevier ◽  
...  
Keyword(s):  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Multidrug Resistant ◽  
Resistant Bacteria ◽  
Multidrug Resistant Bacteria ◽  
Recurrent Urinary Tract Infections ◽  
Gentamicin Treatment ◽  
Tract Infections

scholarly journals Rapid antibiotic susceptibility testing using resazurin bulk modified screen-printed electrochemical sensing platforms

The Analyst ◽  
2021 ◽  
Author(s):  
Benjamin Crane ◽  
Jack Hughes ◽  
Samuel James Rowley-Neale ◽  
Mamun Rashid ◽  
Patrica Linton ◽  
...  
Keyword(s):  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Susceptibility Testing ◽  
Multidrug Resistant ◽  
Electrochemical Sensing ◽  
Resistant Bacteria ◽  
Antibiotic Susceptibility Testing ◽  
Multidrug Resistant Bacteria ◽  
Sensing Platforms ◽  
Tract Infections

Urinary tract infections (UTIs) are one of the most common types of bacterial infection. UTIs can be associated with multidrug resistant bacteria and current methods of determining an effective antibiotic...

scholarly journals Evaluation of the Prevalence of ESBL Multi-Resistant Bacteria, Carbapenemase Producing Enterobacteriaceae in Urinary Tract Infections

2020 ◽  
Vol 17 (6) ◽  
pp. 19-24
Author(s):  
Alina Oana Iacob Cucian ◽  
Septimiu Toader Voidazan ◽  
Nicolae Ovidiu Pop
Keyword(s):  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Multidrug Resistant ◽  
Resistant Bacteria ◽  
Beta Lactamase ◽  
Multidrug Resistant Bacteria ◽  
Extended Spectrum Beta Lactamase ◽  
Cross Border ◽  
Potential Impact ◽  
Tract Infections

Abstract Microbial resistance to antibiotics represents a complex global, cross-border and intergenerational problem that requires interventions in due time, taking into consideration the enormous potential impact on human health. The purpose of this study is to evaluate the prevalence of extended-spectrum beta-lactamase producing bacteria (ESBL), carbapenemase-producing enterobacteriaceae (CPE) multidrug-resistant bacteria in urinary tract infections in the County Emergency Clinical Hospital of Târgu-Mureş during 2017-2018.

scholarly journals Urinary tract infections by multidrug resistant Acinetobacter species- A Retrospective Analysis

2018 ◽  
Vol 08 (03) ◽  
pp. 017-024
Author(s):  
Prashant Mule ◽  
Niranjan Patil ◽  
Seema Gaikwad
Keyword(s):  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Multidrug Resistant ◽  
Hospitalized Patients ◽  
Resistant Bacteria ◽  
Routine Practice ◽  
Polymorphonuclear Leucocytes ◽  
Acinetobacter Species ◽  
Therapeutic Decisions ◽  
Tract Infections

Abstract Background: Acinetobacter species have emerged as important cause of nosocomial infections like pneumonia and urinary tract infections. Acinetobacter species are known to be highly resistant to commonly used antimicrobial agents. SinceAcinetobacter species are ubiquitous and have tremendous colonizing capacity, it is difficult to explain the role of Acinetobacter acquisition in the ICU and medical wards. The objective of this study was to determine the frequency of urinary tract infection (UTI) caused by different Acinetobacter species in hospitalized patients and in the community and to analyze their antimicrobial susceptibility pattern. Materials and Methods: This is a retrospective analysisfrom January 2016 to December 2017. Urine samples collected in appropriate sterile manner were screened for polymorphonuclear leucocytes and bacteria by routine microscopic examination. This was followed by plating on MacConkey's agar and Blood agar. Isolated Acinetobacter strains which are oxidase negative and non-lactose fermenters from MacConkey's agar were identified with Matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) to confirm the TM identification. Antibiotic susceptibility was performed by VitekCompact™ 2 (Biomeuriux, France) as per CLSI standards establishing MIC (Minimum Inhibitory Concentration). Results: Of the 429 isolates of Acinetobacterspecies from urinary tract, Acinetobacterbaumannii complex (78%) found to be the most common species followed by Acinetobacterjunii(10%) and Acinetobacterbaumannii(8%). Of the 334 (78%) isolates of Acinetobacterbaumannii complex from urinary tract, 73 (21.85%) isolates were resistant with doripenem, 62 (18.56%) with imipenem and 59 (17.66%) were resistant with meropenem with MIC values >=8 μg/ml. The rest of the isolates like Acinetobacterjunii, Acinetobacterjohnsonii, andAcinetobacterlwoffiiare found to be more sensitive with carbapenems with lower MIC values. Discussion: The treatment of multidrug-resistant bacteria in hospitalized patients continues to be a challenge for the clinician's in routine practice. Acinetobacter baumannii complex has proven to be an important pathogen in health care associated infections with significant mortality and morbidity. The drug resistant nature of the pathogen and its unpredictable susceptibility patterns make empirical and therapeutic decisions even more difficult. Conclusion: A. baumanniiis an important opportunistic agent of nosocomial UTI, especially in patients with longer hospitalization, antibiotic exposure, urinary catheterization and decreased immunity. High antimicrobial resistance and patient co-morbidities limit therapeutic choices. Hence, alternative therapeutic options are urgently needed to treat a patient with A. baumanniiinfection.

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