Sinapic Acid Protects SH-SY5Y Human Neuroblastoma Cells against 6-Hydroxydopamine-Induced Neurotoxicity

Parkinson’s disease (PD) is characterized by progressive dopaminergic neuron loss or dysfunction and is the second most prevalent neurodegenerative disorder after Alzheimer’s disease. However, current therapeutic strategies for PD are limited to treating the outcomes of this disease rather than preventing it. Sinapic acid (SA) is a phenolic compound with potential antioxidant properties, which reportedly acts as a therapeutic agent against many diseases including cancer, as well as cardiac and liver diseases. However, little is known about the effects of SA against neurodegenerative disorders. Therefore, our study sought to evaluate the neuroprotective effects of non-cytotoxic concentrations of SA against 6-hydroxydopamine (6-OHDA)-induced neurotoxicity in SH-SY5Y human neuroblastoma cells, which we used as an in vitro PD model. SA increased cell viability and rescued the cells from 6-OHDA-induced apoptotic cell death. Additionally, oxidative stress responses were significantly blocked by SA, including reactive oxygen species (ROS) overproduction and decreased expression levels of antioxidant proteins. Notably, SA also attenuated mitochondrial dysfunction and endoplasmic reticulum (ER) stress. Moreover, SA dramatically inhibited the activation of mitogen-activated protein kinase (MAPK) proteins. Taken together, our findings highlight the potential PD prevention effects of SA, as well as its underlying mechanisms, making this compound a promising prevention and treatment agent for PD.

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Tocotrienols protect differentiated SH-SY5Y human neuroblastoma cells against 6-hydroxydopamine-induced cytotoxicity by ameliorating dopamine biosynthesis and dopamine receptor D2 gene expression

Nutrition Research ◽

10.1016/j.nutres.2021.09.003 ◽

2021 ◽

Author(s):

Kasthuri Bai Magalingam ◽

Sushela Devi Somanath ◽

Shadab Md ◽

Nagaraja Haleagrahara ◽

Ju-Yen Fu ◽

...

Keyword(s):

Gene Expression ◽

Dopamine Receptor ◽

Neuroblastoma Cells ◽

Human Neuroblastoma Cells ◽

Human Neuroblastoma ◽

Dopamine Receptor D2 ◽

Dopamine Biosynthesis ◽

6 Hydroxydopamine

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P3-371: Mitochondrial GSH depletion sensitizes human neuroblastoma cells to beta-amyloid peptide-induced oxidative stress and apoptotic cell death

Alzheimer s & Dementia ◽

10.1016/j.jalz.2006.05.1641 ◽

2006 ◽

Vol 2 ◽

pp. S484-S485

Author(s):

Anna Colell ◽

Anna Fernandez ◽

Jose C. Fernandez-Checa

Keyword(s):

Oxidative Stress ◽

Cell Death ◽

Apoptotic Cell ◽

Neuroblastoma Cells ◽

Beta Amyloid ◽

Apoptotic Cell Death ◽

Amyloid Peptide ◽

Human Neuroblastoma Cells ◽

Human Neuroblastoma ◽

Beta Amyloid Peptide

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The Effects of Oleuropein on Different Clinically Types of Human Neuroblastoma Cells

Proceedings ◽

10.3390/proceedings2251591 ◽

2018 ◽

Vol 2 (25) ◽

pp. 1591

Author(s):

Zekiye Altun ◽

Efe Ozgur Serinan ◽

Merve Tütüncü ◽

Safiye Aktaş ◽

Nur Olgun

Keyword(s):

Bone Marrow ◽

Cell Proliferation ◽

Cell Death ◽

Apoptotic Cell ◽

Neuroblastoma Cell ◽

Neuroblastoma Cells ◽

Model Organisms ◽

Late Relapse ◽

Human Neuroblastoma Cells ◽

Human Neuroblastoma

Neuroblastoma is an embryonic tumor originating from the neural crest. It accounts for 8–10% of all childhood cancers. Although Cisplatin is used in neuroblastoma treatment, it has many side effects, such as ototoxicity, nephrotoxicity, and neurotoxicity. One herbal agent that has attracted attention in recent years is oleuropein (OLE), the active component of olive leaf. This component belongs to the polyphenol group and it has antioxidant, anti-microbial, anti-inflammatory, anti-hypertensive and anti-carcinogenic effects. It has beneficial effects against neurodegeneration in both culture cells and model organisms. Oleuropein has been shown to be increased apoptosis in SH-SY5Y neuroblastoma cell line in one study. Cisplatin (cis-diaminedichloroplatinum II (CDDP) is a widely used agent for the treatment of many different human cancers in childhood and adults with antimitotic and antineoplastic properties. CDDP is the most effective chemotherapeutic agent in specially treatment of neuroblastoma. Purpose of this study was to determine whether oleuropein and CDDP have possible anti-proliferative activity in different types of human neuroblastoma cells as representing different clinical features (bone marrow metastatic LAN-5 cells and treated with chemotherapy and beam therapy CHP-134 cells representing late relapse) investigated. Human bone marrow metastatic LAN-5 and treated with chemotherapy and beam therapy CHP-134 neuroblastoma cells representing late relapse were used in this study. The effects of OLE and CDDP on LAN-5 and CHP-134 neuroblastoma cell proliferation and apoptotic cell death was investigated using WST-1 cell proliferation and Annexin-V/PI flow cytometric assays. Oleuropein and CDDP have been shown to inhibit proliferation of LAN-5 and CHP-134 neuroblastoma cells. In further studies, it is planned to investigate different cell death mechanisms by using combination of oleuropein and cisplatin in different kind of human neuroblastoma cells.

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