scholarly journals Ultra-Small Superparamagnetic Iron-Oxide Nanoparticles Exert Different Effects on Erythrocytes in Normotensive and Hypertensive Rats

Biomedicines ◽  
2021 ◽  
Vol 9 (4) ◽  
pp. 377
Author(s):  
Jana Radosinska ◽  
Tomas Jasenovec ◽  
Dominika Radosinska ◽  
Peter Balis ◽  
Angelika Puzserova ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Superparamagnetic Iron Oxide ◽  
Erythrocyte Deformability ◽  
Oxide Nanoparticles ◽  
Nitric Oxide Production ◽  
Hypertensive Rats ◽  
Oxide Production ◽  
Nominal Dose

We determined erythrocyte physiological and biochemical properties after the single and repeated administration of ultra-small superparamagnetic iron-oxide nanoparticles (USPIONs) in normotensive Wistar–Kyoto (WKY) and spontaneously hypertensive (SHR) rats. Polyethylene glycol-coated USPIONs (transmission electron microscope detected a mean size of ~30 nm and hydrodynamic size ~51 nm) were intravenously administered to rats either in one infusion at nominal dose 1 mg Fe/kg or in two infusions (administered with a difference of 24 h) at nominal dose 2 mg Fe/kg. Results showed that USPIONs did not deteriorate erythrocyte deformability, nitric oxide production, and osmotic resistance in both experimental settings. Both the single and repeated USPION administration elevated erythrocyte deformability in WKY. However, this effect was not present in SHR; deformability in USPION-treated SHR was significantly lower than in USPION-treated WKY. Nitric oxide production by erythrocytes was increased after a single USPION treatment in WKY, so it can be associated with improvement in erythrocyte deformability. Using biomagnetometry, we revealed significantly lower amounts of USPION-originated iron in erythrocytes in SHR compared with WKY. We found a much faster elimination of USPIONs from erythrocytes in hypertensive rats compared with the normotensive ones, which might be relevant for clinical practice in hypertensive patients undergoing clinical examination with the use of iron-oxide nanoparticles.

Nitric oxide donor superparamagnetic iron oxide nanoparticles

2013 ◽  
Vol 33 (2) ◽  
pp. 746-751 ◽  
Author(s):  
Miguel M. Molina ◽  
Amedea B. Seabra ◽  
Marcelo G. de Oliveira ◽  
Rosangela Itri ◽  
Paula S. Haddad
Keyword(s):  
Nitric Oxide ◽  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Superparamagnetic Iron Oxide ◽  
Superparamagnetic Iron Oxide Nanoparticles ◽  
Nitric Oxide Donor ◽  
Oxide Nanoparticles

scholarly journals Superparamagnetic iron oxide nanoparticles dispersed in Pluronic F127 hydrogel: potential uses in topical applications

RSC Advances ◽  
2017 ◽  
Vol 7 (24) ◽  
pp. 14496-14503 ◽  
Author(s):  
L. C. Gonçalves ◽  
A. B. Seabra ◽  
M. T. Pelegrino ◽  
D. R. de Araujo ◽  
J. S. Bernardes ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Superparamagnetic Iron Oxide ◽  
Superparamagnetic Iron Oxide Nanoparticles ◽  
Pluronic F127 ◽  
Synthesis And Characterization ◽  
Oxide Nanoparticles ◽  
Topical Applications

The present study is focused on the synthesis and characterization of nitric oxide (NO)-releasing superparamagnetic iron oxide nanoparticles, and their incorporation in Pluronic F127 hydrogel with great potential for topical applications.

Nitric oxide (NO) releasing-superparamagnetic iron oxide nanoparticles for biomedical applications

Nitric Oxide ◽  
2012 ◽  
Vol 27 ◽  
pp. S38
Author(s):  
Amedea B. Seabra ◽  
Paula S. Haddad ◽  
Miguel M. Molina ◽  
Rosangela Itri ◽  
Marcelo G. de Oliveira
Keyword(s):  
Nitric Oxide ◽  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Biomedical Applications ◽  
Superparamagnetic Iron Oxide ◽  
Superparamagnetic Iron Oxide Nanoparticles ◽  
Oxide Nanoparticles

TARGETING CYCLIN B1 WITH ANTISENSE OLIGONUCLETOTIDES- COATED SUPERPARAMAGNETIC IRON OXIDE NANOPARTICLES

2018 ◽  
Vol 6 (10) ◽  
Author(s):  
Hosam Zaghloul ◽  
Doaa A. Shahin ◽  
Ibrahim El- Dosoky ◽  
Mahmoud E. El-awady ◽  
Fardous F. El-Senduny ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Cellular Uptake ◽  
Superparamagnetic Iron Oxide ◽  
Cyclin B1 ◽  
Superparamagnetic Iron Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Mcf7 Cells ◽  
M Phase ◽  
The Impact

Antisense oligonucleotides (ASO) represent an attractive trend as specific targeting molecules but sustain poor cellular uptake meanwhile superparamagnetic iron oxide nanoparticles (SPIONs) offer stability of ASO and improved cellular uptake. In the present work we aimed to functionalize SPIONs with ASO targeting the mRNA of Cyclin B1 which represents a potential cancer target and to explore its anticancer activity. For that purpose, four different SPIONs-ASO conjugates, S-M (1–4), were designated depending on the sequence of ASO and constructed by crosslinking carboxylated SPIONs to amino labeled ASO. The impact of S-M (1–4) on the level of Cyclin B1, cell cycle, ROS and viability of the cells were assessed by flowcytometry. The results showed that S-M3 and S-M4 reduced the level of Cyclin B1 by 35 and 36%, respectively. As a consequence to downregulation of Cyclin B1, MCF7 cells were shown to be arrested at G2/M phase (60.7%). S-M (1–4) led to the induction of ROS formation in comparison to the untreated control cells. Furthermore, S-M (1–4) resulted in an increase in dead cells compared to the untreated cells and SPIONs-treated cells. In conclusion, targeting Cyclin B1 with ASO-coated SPIONs may represent a specific biocompatible anticancer strategy.

Evaluation of Surface-modified Superparamagnetic Iron Oxide Nanoparticles to Optimize Bacterial Immobilization for Bio-separation with the Least Inhibitory Effect on Microorganism Activity

2020 ◽  
Vol 10 (2) ◽  
pp. 166-174
Author(s):  
Mehdi Khoshneviszadeh ◽  
Sarah Zargarnezhad ◽  
Younes Ghasemi ◽  
Ahmad Gholami
Keyword(s):  
Iron Oxide ◽  
Amino Acid ◽  
Magnetic Nanoparticles ◽  
Iron Oxide Nanoparticles ◽  
Surface Coating ◽  
Superparamagnetic Iron Oxide ◽  
Superparamagnetic Iron Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Surface Charges ◽  
Surface Modified

Background: Magnetic cell immobilization has been introduced as a novel, facile and highly efficient approach for cell separation. A stable attachment between bacterial cell wall with superparamagnetic iron oxide nanoparticles (SPIONs) would enable the microorganisms to be affected by an outer magnetic field. At high concentrations, SPIONs produce reactive oxygen species in cytoplasm, which induce apoptosis or necrosis in microorganisms. Choosing a proper surface coating could cover the defects and increase the efficiency. Methods: In this study, asparagine, APTES, lipo-amino acid and PEG surface modified SPIONs was synthesized by co-precipitation method and characterized by FTIR, TEM, VSM, XRD, DLS techniques. Then, their protective effects against four Gram-positive and Gram-negative bacterial strains including Enterococcus faecalis, Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa were examined through microdilution broth and compared to naked SPION. Results: The evaluation of characterization results showed that functionalization of magnetic nanoparticles could change their MS value, size and surface charges. Also, the microbial analysis revealed that lipo-amino acid coated magnetic nanoparticles has the least adverse effect on microbial strain among tested SPIONs. Conclusion: This study showed lipo-amino acid could be considered as the most protective and even promotive surface coating, which is explained by its optimizing effect on cell penetration and negligible reductive effects on magnetic properties of SPIONs. lipo-amino acid coated magnetic nanoparticles could be used in microbial biotechnology and industrial microbiology.

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