scholarly journals Development of a Sensitive Self-Powered Glucose Biosensor Based on an Enzymatic Biofuel Cell

Biosensors ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 16
Author(s):  
Kantapat Chansaenpak ◽  
Anyanee Kamkaew ◽  
Sireerat Lisnund ◽  
Pannaporn Prachai ◽  
Patipat Ratwirunkit ◽  
...  
Keyword(s):  
Carbon Nanostructures ◽  
Glucose Sensor ◽  
Glucose Dehydrogenase ◽  
Food Samples ◽  
Glucose Biosensor ◽  
Biofuel Cell ◽  
Multi Wall Carbon Nanotubes ◽  
Gas Purge ◽  
External Power Source ◽  
Self Powered

Biofuel cells allow for constructing sensors that leverage the specificity of enzymes without the need for an external power source. In this work, we design a self-powered glucose sensor based on a biofuel cell. The redox enzymes glucose dehydrogenase (NAD-GDH), glucose oxidase (GOx), and horseradish peroxidase (HRP) were immobilized as biocatalysts on the electrodes, which were previously engineered using carbon nanostructures, including multi-wall carbon nanotubes (MWCNTs) and reduced graphene oxide (rGO). Additional polymers were also introduced to improve biocatalyst immobilization. The reported design offers three main advantages: (i) by using glucose as the substrate for the both anode and cathode, a more compact and robust design is enabled, (ii) the system operates under air-saturating conditions, with no need for gas purge, and (iii) the combination of carbon nanostructures and a multi-enzyme cascade maximizes the sensitivity of the biosensor. Our design allows the reliable detection of glucose in the range of 0.1–7.0 mM, which is perfectly suited for common biofluids and industrial food samples.

scholarly journals Detection of Human Plasma Glucose Using a Self-Powered Glucose Biosensor

Energies ◽  
2019 ◽  
Vol 12 (5) ◽  
pp. 825 ◽  
Author(s):  
Gymama Slaughter ◽  
Tanmay Kulkarni
Keyword(s):  
Human Plasma ◽  
Power Density ◽  
Plasma Glucose ◽  
Cell Voltage ◽  
Glucose Biosensor ◽  
Biofuel Cell ◽  
Diabetic Patients ◽  
Glucose Levels ◽  
External Power Source ◽  
Self Powered

This work presents the characterization of a self-powered glucose biosensor using individual sequential assays of human plasma glucose obtained from diabetic patients. The self-powered glucose biosensor is exploited to optimize the assay parameters for sensing plasma glucose levels. In particular, the biofuel cell component of the system at pH 7.4, 37 °C generates a power density directly proportional to plasma glucose and exhibited a maximum power density of 0.462 mW·cm−2 at a cell voltage of 0.213 V in 5 mM plasma glucose. Plasma glucose is further sensed by monitoring the charge/discharge frequency (Hz) of the integrated capacitor functioning as the transducer. With this method, the plasma glucose is quantitatively detected in 100 microliters of human plasma with unprecedented sensitivity, as high as 104.51 ± 0.7 Hz·mM−1·cm−2 and a detection limit of 2.31 ± 0.3 mM. The results suggest the possibility to sense human plasma glucose at clinically relevant concentrations without the use of an external power source.

Three-Dimensional Glucose/Oxygen Biofuel Cells Based on Enzymes Embedded in Tetrabutylammonium Modified Nafion

2021 ◽  
Vol 18 (4) ◽  
Author(s):  
Yuchen Hui ◽  
Xiaoyan Ma ◽  
Rong Cai ◽  
Shelley D. Minteer
Keyword(s):  
Direct Electron Transfer ◽  
Glucose Dehydrogenase ◽  
Tetrabutylammonium Bromide ◽  
Three Dimensional ◽  
Phosphate Buffer Solution ◽  
Open Circuit ◽  
Biofuel Cell ◽  
Buffer Solution ◽  
Multi Wall Carbon Nanotubes ◽  
Enzymatic Biofuel Cell

Abstract A stable three-dimensional glucose/oxygen enzymatic biofuel cell is fabricated based on the method of polymer encapsulation-based immobilization. And three-dimensional carbon felt is used as the substrate of the bio-electrode for increasing enzymatic loading density. Gold nanoparticles and multi-wall carbon nanotubes are employed to promote direct electron transfer and enhance conductivity and electron conduction rate of bio-electrodes. Glucose dehydrogenase and bilirubin oxidase are immobilized with tetrabutylammonium bromide (TBAB) modified Nafion, which enhances the stability of the bio-electrodes by the immobilization method. A membrane-free glucose/oxygen biofuel cell is assembled with a high open-circuit voltage of 0.85 V and a maximum power density of 21.9 ± 0.1 μW/cm2 in 0.1 M pH 7.0 phosphate buffer solution with 100 mM glucose and air saturation. And the biofuel cell shows high stability to the condition. After 60 days of periodic storage experiments, the performance of the enzymatic biofuel cell still maintained 90.3% of its electrochemical performance.

scholarly journals Paper-Based Disk-Type Self-Powered Glucose Biosensor Based on Screen-Printed Biofuel Cell Array

2019 ◽  
Vol 166 (12) ◽  
pp. B1063-B1068 ◽  
Author(s):  
Isao Shitanda ◽  
Yuki Fujimura ◽  
Saki Nohara ◽  
Yoshinao Hoshi ◽  
Masayuki Itagaki ◽  
...  
Keyword(s):  
Glucose Biosensor ◽  
Biofuel Cell ◽  
Cell Array ◽  
Self Powered ◽  
Screen Printed

A mediator-free self-powered glucose biosensor based on a hybrid glucose/MnO2 enzymatic biofuel cell

Nano Research ◽  
2020 ◽  
Vol 14 (3) ◽  
pp. 707-714
Author(s):  
Shuai Hao ◽  
He Zhang ◽  
Xiaoxuan Sun ◽  
Junfeng Zhai ◽  
Shaojun Dong
Keyword(s):  
Glucose Biosensor ◽  
Biofuel Cell ◽  
Enzymatic Biofuel Cell ◽  
Self Powered

scholarly journals A quasi-solid-state and self-powered biosupercapacitor based on flexible nanoporous gold electrodes

2018 ◽  
Vol 54 (46) ◽  
pp. 5823-5826 ◽  
Author(s):  
Xinxin Xiao ◽  
Edmond Magner
Keyword(s):  
Solid State ◽  
Nanoporous Gold ◽  
Biofuel Cell ◽  
Gold Electrodes ◽  
Self Powered

A quasi-solid-state and flexible biofuel cell using a hydrogel electrolyte preloaded with sugar as a fuel is described.

In Vitro Evaluation of Miniaturized Amperometric Enzyme Sensor Based on the Direct Electron Transfer Principle for Continuous Glucose Monitoring

2022 ◽  
pp. 193229682110706
Author(s):  
Yutaro Inoue ◽  
Yasuhide Kusaka ◽  
Kotaro Shinozaki ◽  
Inyoung Lee ◽  
Koji Sode
Keyword(s):  
Electron Transfer ◽  
Continuous Glucose Monitoring ◽  
Glucose Sensor ◽  
Direct Electron Transfer ◽  
Glucose Dehydrogenase ◽  
Glucose Monitoring ◽  
Reference Electrode ◽  
Enzyme Sensor

Background: The bacterial derived flavin adenine dinucleotide (FAD)-dependent glucose dehydrogenase (FADGDH) is the most promising enzyme for the third-generation principle-based enzyme sensor for continuous glucose monitoring (CGM). Due to the ability of the enzyme to transfer electrons directly to the electrode, recognized as direct electron transfer (DET)-type FADGDH, although no investigation has been reported about DET-type FADGDH employed on a miniaturized integrated electrode. Methods: The miniaturized integrated electrode was formed by sputtering gold (Au) onto a flexible film with 0.1 mm in thickness and divided into 3 parts. After an insulation layer was laminated, 3 openings for a working electrode, a counter electrode and a reference electrode were formed by dry etching. A reagent mix containing 1.2 × 10−4 Unit of DET-type FADGDH and carbon particles was deposited. The long-term stability of sensor was evaluated by continuous operation, and its performance was also evaluated in the presence of acetaminophen and the change in oxygen partial pressure (pO2) level. Results: The amperometric response of the sensor showed a linear response to glucose concentration up to 500 mg/dL without significant change of the response over an 11-day continuous measurement. Moreover, the effect of acetaminophen and pO2 on the response were negligible. Conclusions: These results indicate the superb potential of the DET-type FADGDH-based sensor with the combination of a miniaturized integrated electrode. Thus, the described miniaturized DET-type glucose sensor for CGM will be a promising tool for effective glycemic control. This will be further investigated using an in vivo study.

Mathematical Modeling Of An Amperometric Glucose Sensor: The Effect Of Membrane Permeability And Selectivity On Performance

2012 ◽  
Author(s):  
Azila Abd. Aziz
Keyword(s):  
Mathematical Modeling ◽  
Transport Properties ◽  
Membrane Permeability ◽  
Glucose Sensor ◽  
Digital Simulation ◽  
Glucose Biosensor ◽  
Glucose Biosensors ◽  
Interference Reduction ◽  
Membrane Selectivity ◽  
Selective Membrane

Gangguan dari bahan kimia elektro–aktif seperti asid askorbik, asid urik dan asetaminofen adalah merupakan satu masalah bagi biosensor glukosa berasaskan peroksid. Kebanyakan kerja penyelidikan memfokuskan kepada penggunaan membran yang perm–selektif di antara elektrod dan komponen aktif sensor untuk menghilangkan masalah ini. Dalam kerja penyelidikan ini, satu model matematik telah dibina untuk mengkaji kesan kebolehtelapan dan kememilihan bagi prestasi biosensor glukosa berasaskan peroksid. Simulasi digital telah dijalankan menggunakan kaedah pembezaan terhingga. Seperti yang dijangka, kememilihan membran kepada peroksid memainkan peranan besar dalam mengurangkan gangguan. Namun begitu, model juga mencadangkan yang manipulasi sifat pengangkutan lapisan pelindung luar boleh juga menghasilkan keputusan yang memberangsangkan dalam mengurangkan gangguan. Kata kunci: Biosensor glukosa; model matematik; asetaminofen; pengganggu; sifat pengangkutan Interference from electro–active chemicals such as ascorbic acid, uric acid and acetaminophen can be a problem for peroxide based glucose biosensors. Most works focused on the employment of a perm–selective membrane sandwiched between the electrode and the active component of the sensor to overcome this problem. In this work, a mathematical model has been developed to study the effect of membrane permeability and selectivity on peroxide based glucose biosensor performance. Digital simulation was carried out using the finite difference method. As expected, membrane selectivity to peroxide played a major role in interference reduction. However, interestingly, the model also suggested that the manipulation of the transport properties of the protective outer layer would also result in acceptable interference reduction. Key words: Glucose biosensors; mathematical modeling; acetaminophen; interferents; transport properties

scholarly journals A Self-Powered Biosensor for the Detection of Glutathione

Biosensors ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 114 ◽  
Author(s):  
Brandon G. Roy ◽  
Julia L. Rutherford ◽  
Anna E. Weaver ◽  
Kevin Beaver ◽  
Michelle Rasmussen
Keyword(s):  
Detection Limit ◽  
Glucose Oxidase ◽  
Power Output ◽  
Low Cost ◽  
Linear Range ◽  
Biofuel Cell ◽  
Bilirubin Oxidase ◽  
Biological Molecule ◽  
Enzymatic Biofuel Cell ◽  
Self Powered

Glutathione is an important biological molecule which can be an indicator of numerous diseases. A method for self-powered detection of glutathione levels in solution has been developed using an enzymatic biofuel cell. The device consists of a glucose oxidase anode and a bilirubin oxidase cathode. For the detection of glutathione, the inhibition of bilirubin oxidase leads to a measurable decrease in current and power output. The reported method has a detection limit of 0.043 mM and a linear range up to 1.7 mM. Being able to detect a range of concentrations can be useful in evaluating a patient’s health. This method has the potential to be implemented as a quick, low-cost alternative to previously reported methods.

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