Modifications of the PAMONO-Sensor Help to Size and Quantify Low Number of Individual Biological and Non-Biological Nano-Particles

In a series of recently published works, we demonstrated that the plasmon-assisted microscopy of nano-objects (PAMONO) technique can be successfully employed for the sizing and quantification of single viruses, virus-like particles, microvesicles and charged non-biological particles. This approach enables label-free, but specific detection of biological nano-vesicles. Hence, the sensor, which was built up utilizing plasmon-assisted microscopy, possesses relative versatility and it can be used as a platform for cell-based assays. However, one of the challenging tasks for such a sensor was the ability to reach a homogeneous illumination of the whole surface of the gold sensor slide. Moreover, in order to enable the detection of even relatively low concentrations of nano-particles, the focused image area had to be expanded. Both tasks were solved via modifications of previously described PAMONO-sensor set ups. Taken together, our latest findings can help to develop a research and diagnostic platform based on the principles of the surface plasmon resonance (SPR)-assisted microscopy of nano-objects.

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Label-free and specific detection of soluble programmed death ligand-1 using a localized surface plasmon resonance biosensor based on excessively tilted fiber gratings

Biomedical Optics Express ◽

10.1364/boe.10.005136 ◽

2019 ◽

Vol 10 (10) ◽

pp. 5136

Author(s):

Binbin Luo ◽

Yajie Wang ◽

Huafeng Lu ◽

Shengxi Wu ◽

Youming Lu ◽

...

Keyword(s):

Surface Plasmon Resonance ◽

Surface Plasmon ◽

Plasmon Resonance ◽

Localized Surface Plasmon Resonance ◽

Localized Surface Plasmon ◽

Label Free ◽

Specific Detection ◽

Fiber Gratings ◽

Programmed Death Ligand 1 ◽

Programmed Death

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Plasmonic Optical Biosensors for Detecting C-Reactive Protein: A Review

Micromachines ◽

10.3390/mi11100895 ◽

2020 ◽

Vol 11 (10) ◽

pp. 895

Author(s):

Joo Seon Seok ◽

Heongkyu Ju

Keyword(s):

Surface Plasmon Resonance ◽

Surface Plasmon ◽

Plasmon Resonance ◽

Protein A ◽

Localized Surface Plasmon ◽

Label Free ◽

C Reactive Protein ◽

Specific Detection ◽

Detection Range ◽

Reactive Protein

C-reactive protein (CRP), a potent acute-phase reactant that increases rapidly in response to inflammation, tissue damage or infections, is also considered an indicator of the risk of cardiovascular diseases and neurological disorders. Recent advances in nanofabrication and nanophotonic technologies have prompted the optical plasmonic phenomena to be tailored for specific detection of human serum CRP into label-free devices. We review the CRP-specific detection platforms with high sensitivity, which feature the thin metal films for surface plasmon resonance, nano-enhancers of zero dimensional nanostructures, and metal nanoparticles for localized surface plasmon resonance. The protocols used for various types of assay reported in literature are also outlines with surface chemical pretreatment required for specific detection of CRPs on a plasmonic surface. Properties including sensitivity and detection range are described for each sensor device reviewed, while challenges faced by plasmonic CRP sensors are discussed in the conclusion, with future directions towards which research efforts need to be made.

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Plasma enhanced label-free immunoassay for alpha-fetoprotein based on a U-bend fiber-optic LSPR biosensor

RSC Advances ◽

10.1039/c5ra02910d ◽

2015 ◽

Vol 5 (31) ◽

pp. 23990-23998 ◽

Cited By ~ 37

Author(s):

Gaoling Liang ◽

Zhongjun Zhao ◽

Yin Wei ◽

Kunping Liu ◽

Wenqian Hou ◽

...

Keyword(s):

Surface Plasmon Resonance ◽

Surface Plasmon ◽

Plasmon Resonance ◽

Localized Surface Plasmon Resonance ◽

Fiber Optic ◽

Cost Effective ◽

Alpha Fetoprotein ◽

Localized Surface Plasmon ◽

Label Free

A simple, label-free and cost-effective localized surface plasmon resonance (LSPR) immunosensing method was developed for detection of alpha-fetoprotein (AFP).

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Trends in SPR Cytometry: Advances in Label-Free Detection of Cell Parameters

Biosensors ◽

10.3390/bios8040102 ◽

2018 ◽

Vol 8 (4) ◽

pp. 102 ◽

Cited By ~ 8

Author(s):

Richard Schasfoort ◽

Fikri Abali ◽

Ivan Stojanovic ◽

Gestur Vidarsson ◽

Leon Terstappen

Keyword(s):

Surface Plasmon Resonance ◽

Surface Plasmon ◽

Plasmon Resonance ◽

Mammalian Cells ◽

Living Cells ◽

Label Free ◽

Intact Cells ◽

Cell Parameters ◽

Spr Imaging ◽

Label Free Detection

SPR cytometry entails the measurement of parameters from intact cells using the surface plasmon resonance (SPR) phenomenon. Specific real-time and label-free binding of living cells to sensor surfaces has been made possible through the availability of SPR imaging (SPRi) instruments and researchers have started to explore its potential in the last decade. Here we will discuss the mechanisms of detection and additionally describe the problems and issues of mammalian cells in SPR biosensing, both from our own experience and with information from the literature. Finally, we build on the knowledge and applications that has already materialized in this field to give a forecast of some exciting applications for SPRi cytometry.

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Rapid Antibody Selection Using Surface Plasmon Resonance for High-Speed and Sensitive Hazelnut Lateral Flow Prototypes

Biosensors ◽

10.3390/bios8040130 ◽

2018 ◽

Vol 8 (4) ◽

pp. 130 ◽

Cited By ~ 6

Author(s):

Georgina Ross ◽

Maria Bremer ◽

Jan Wichers ◽

Aart van Amerongen ◽

Michel Nielen

Keyword(s):

Surface Plasmon Resonance ◽

Surface Plasmon ◽

Plasmon Resonance ◽

High Speed ◽

Selection Process ◽

Low Cost ◽

Real Life ◽

Cross Reactivity ◽

Lateral Flow ◽

Label Free

Lateral Flow Immunoassays (LFIAs) allow for rapid, low-cost, screening of many biomolecules such as food allergens. Despite being classified as rapid tests, many LFIAs take 10–20 min to complete. For a really high-speed LFIA, it is necessary to assess antibody association kinetics. By using a label-free optical technique such as Surface Plasmon Resonance (SPR), it is possible to screen crude monoclonal antibody (mAb) preparations for their association rates against a target. Herein, we describe an SPR-based method for screening and selecting crude anti-hazelnut antibodies based on their relative association rates, cross reactivity and sandwich pairing capabilities, for subsequent application in a rapid ligand binding assay. Thanks to the SPR selection process, only the fast mAb (F-50-6B12) and the slow (S-50-5H9) mAb needed purification for labelling with carbon nanoparticles to exploit high-speed LFIA prototypes. The kinetics observed in SPR were reflected in LFIA, with the test line appearing within 30 s, almost two times faster when F-50-6B12 was used, compared with S-50-5H9. Additionally, the LFIAs have demonstrated their future applicability to real life samples by detecting hazelnut in the sub-ppm range in a cookie matrix. Finally, these LFIAs not only provide a qualitative result when read visually, but also generate semi-quantitative data when exploiting freely downloadable smartphone apps.

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