scholarly journals Sequential Isolation and Characterization of Single CTCs and Large CTC Clusters in Metastatic Colorectal Cancer Patients

Cancers ◽  
2021 ◽  
Vol 13 (24) ◽  
pp. 6362
Author(s):  
Federica Francescangeli ◽  
Valentina Magri ◽  
Maria Laura De Angelis ◽  
Gianluigi De Renzi ◽  
Orietta Gandini ◽  
...  

Circulating tumor cells (CTCs) detach from a primary tumor or its metastases and circulate in the bloodstream. The vast majority of CTCs are deemed to die into the bloodstream, with only few cells representing viable metastatic precursors. Particularly, single epithelial CTCs do not survive long in the circulation due to the loss of adhesion-dependent survival signals. In metastatic colorectal cancer, the generation of large CTC clusters is a very frequent occurrence, able to increase the aptitude of CTCs to survive in the bloodstream. Although a deepened analysis of large-sized CTC clusters might certainly offer new insights into the complexity of the metastatic cascade, most CTC isolation techniques are unfortunately not compatible with large-sized CTC clusters isolation. The inappropriateness of standard CTC isolation devices for large clusters isolation and the scarce availability of detection methods able to specifically isolate and characterize both single CTCs and CTC clusters finally prevented in-depth studies on the prognostic and predictive value of clusters in clinical practice, unlike that which has been described for single CTCs. In the present study, we validated a new sequential filtration method for the simultaneous isolation of large CTC clusters and single CTCs in patients with metastatic colorectal cancer at failure of first-line treatments. The new method might allow differential downstream analyses for single and clustered CTCs starting from a single blood draw, opening new scenarios for an ever more precise characterization of colorectal cancer metastatic cascade.

2006 ◽  
Vol 6 (2) ◽  
pp. 125-132 ◽  
Author(s):  
Steven J. Cohen ◽  
R. Katherine Alpaugh ◽  
Steve Gross ◽  
Shawn M. O'Hara ◽  
Denis A. Smirnov ◽  
...  

BMC Cancer ◽  
2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Ruwan Parakrama ◽  
Elisha Fogel ◽  
Carol Chandy ◽  
Titto Augustine ◽  
Matt Coffey ◽  
...  

2015 ◽  
Vol 34 (5) ◽  
pp. 2768-2775 ◽  
Author(s):  
MAŁGORZATA STEC ◽  
MONIKA BAJ-KRZYWORZEKA ◽  
JAROSŁAW BARAN ◽  
KAZIMIERZ WĘGLARCZYK ◽  
MARIA ZEMBALA ◽  
...  

2017 ◽  
Vol 63 (2) ◽  
pp. 165-169 ◽  
Author(s):  
S.N. Tamkovich ◽  
N.V. Yunusova ◽  
M.N. Stakheeva ◽  
A.K. Somov ◽  
A.Y. Frolova ◽  
...  

A simple approach for isolation of exosomes from the blood plasma, which allows to obtain highly purified preparations of microvesicles no larger than 100 nm has been proposed. The presence of different subpopulations of exosomes in the blood plasma of healthy donors and cancer patients has been recognized. We found the presence of the universal markers CD9, CD24 and CD81 on exosomes isolated from blood plasma that can be used to their routine typing.


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