scholarly journals Improvement of Cell Growth of Uterosacral Ligament Fibroblast Derived from Pelvic Organ Prolapse Patients by Cold Atmospheric Plasma Treated Liquid

Cells ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 2728
Author(s):  
Ihn Han ◽  
Seung Ah Choi ◽  
Seul I Kim ◽  
Eun Ha Choi ◽  
Young Joo Lee ◽  
...  
Keyword(s):  
Pelvic Organ Prolapse ◽  
Cell Viability ◽  
Cell Apoptosis ◽  
Atmospheric Pressure Plasma ◽  
Pelvic Organ ◽  
Uterosacral Ligament ◽  
Atmospheric Plasma ◽  
Skov3 Cells ◽  
Plasma Device ◽  
Barrier Discharge Plasma

Pelvic organ prolapse (POP) is a chronic disorder that affects quality of life in women. Several POP treatments may be accompanied by abrasion, constant infection, and severe pain. Therefore, new treatment methods and improvements in current treatments for POP are required. Non-thermal atmospheric-pressure plasma is a rising biomedical therapy that generates a mixed cocktail of reactive species by different mechanisms. In this study, we applied a cylinder-type dielectric barrier discharge plasma device to create a plasma-treated liquid (PTL). The PTL was added to primary cultured human uterosacral ligament fibroblast (hUSLF) cells from POP patients at each stage. Surprisingly, treatment with diluted PTL increased hUSLF cell viability but decreased ovarian cancer cell viability. PTL also decreased cell apoptosis in hUSLF cells but increased it in SKOV3 cells. Our results suggest that PTL protects hUSLF cells from cell apoptosis by controlling the p53 pathway and improves cell viability, implying that PTL is a promising application for POP therapy.

scholarly journals IGF-1 regulates the growth of fibroblasts and extracellular matrix deposition in pelvic organ prolapse

Open Medicine ◽  
2020 ◽  
Vol 15 (1) ◽  
pp. 833-840
Author(s):  
Yitong Yin ◽  
Ying Han ◽  
Chang Shi ◽  
Zhijun Xia
Keyword(s):  
Pelvic Organ Prolapse ◽  
Cell Viability ◽  
Cell Apoptosis ◽  
Nuclear Factor Κb ◽  
Pelvic Organ ◽  
Vaginal Wall ◽  
Western Blot Assay ◽  
Matrix Deposition ◽  
Thiazolyl Blue Tetrazolium Bromide ◽  
The Impact

AbstractThis study was carried out to observe the impact of insulin-like growth factor-1 (IGF-1) on human vaginal fibroblasts (HVFs) in the context of pelvic organ prolapse (POP) and to explore its effects on mitogen-activated protein kinases (MAPK) and nuclear factor-κB (NF-κB) signaling pathways. First, it was found that IGF-1 expression reduced in the vaginal wall tissues derived from POP compared to that in non-POP cases. Then the role of IGF-1 was explored in HVFs and thiazolyl blue tetrazolium bromide (MTT) and flow cytometry were used to detect cell viability and cell apoptosis. Western blot assay and quantitative real-time polymerase chain reaction were used to detect the protein and mRNA expression. The results showed that knockdown of IGF-1 inhibited the cell viability of HVFs, promoted the cell apoptosis of HVFs, and decreased the expression of types I and III collagen in HVFs, which was through inhibiting the expression of IGF-1 receptor and MAPK/NF-κB pathways. However, IGF-1 plasmid had the opposite effects on HVFs. In conclusion, our results showed that IGF-1 could activate MAPK and NF-κB pathways, thereby enhancing collagen metabolism and the growth of vaginal wall fibroblasts then to inhibit POP development.

scholarly journals To Compare the Effectiveness of Vaginal High Uterosacral Ligament Suspension and McCall's Culdoplasty during Vaginal Hysterectomy for Pelvic Organ Prolapse

2017 ◽  
Vol 5 (2) ◽  
pp. 81-86
Author(s):  
Krutika Bhalerao ◽  
Anuja V Bhalerao ◽  
Richa Garg
Keyword(s):  
Pelvic Organ Prolapse ◽  
Coming Out ◽  
Vaginal Hysterectomy ◽  
Pelvic Organ ◽  
Uterosacral Ligament ◽  
Vaginal Vault ◽  
Care Hospital ◽  
Uterosacral Ligament Suspension ◽  
Vaginal Length ◽  
Vault Suspension

ABSTRACT Introduction Vaginal vault prolapse can be prevented by supporting the vaginal cuff, which is an essential part of hysterectomy, whether done abdominally or vaginally. The American Association of Gynecologic Laparoscopists (AAGL) has recommended for future research, specifically, a randomized trial comparing McCall's culdoplasty (with uterosacral ligament plication) with vaginal high uterosacral ligament suspension (HUSLS) (without plication), since both procedures are accessible to gynecological surgeons without urologic background. Hence, this study was carried out. Aim To compare both anatomic and functional outcomes of patients undergoing vaginal HUSLS or McCall's culdoplasty at the time of vaginal hysterectomy. Materials and methods This hospital-based prospective comparative study was carried out at a tertiary care hospital from January 1, 2013 to December 31, 2015 over a period of 3 years after obtaining Ethical Committee approval. All women attending gynecological outpatient department having symptom of mass coming out of vagina were subjected to detailed history, examination, and later underwent either HUSLS (43) or McCall's culdoplasty (42), for vault suspension with concomitant hysterectomy. The effectiveness of both the procedures was assessed by preoperative and postoperative pelvic organ prolapse quantification (POP-Q) and both were compared. Observations There was statistically significant improvement in all the sites of POP-Q points by HUSLS and McCall's culdoplasty as a method of vault suspension except in total vaginal length (TVL). Vault suspension by HUSLS is better than McCall's culdoplasty. All the points of POP-Q showed better results but the point C was significantly placed at a higher level by HUSLS (p = 0.000) as compared with McCall's culdoplasty. The time required for HUSLS was statistically more as compared with repair by McCall's culdoplasty (81.55/74.53 minutes, T: 1.981, p: 0.05). Complications, such as hemorrhage and ureteric injuries were more in HUSLS (2/43, 4.8%) as compared with McCall's culdoplasty (0/42); this is statistically significant. Conclusion High uterosacral ligament suspension provides excellent suspensory support to vaginal vault. Vagina is suspended over the levator ani with normal axis toward sacrum. By doing HUSLS, the vagina is symmetrically supported directed toward the hollow of sacrum. High uterosacral ligament suspension is highly recommended for young women with POP as vaginal length is not altered at all and so is the quality of life. How to cite this article Bhalerao AV, Bhalerao K, Garg R. To Compare the Effectiveness of Vaginal High Uterosacral Ligament Suspension and McCall's Culdoplasty during Vaginal Hysterectomy for Pelvic Organ Prolapse. J South Asian Feder Menopause Soc 2017;5(2):81-86.

scholarly journals Proteomic Analysis of the Uterosacral Ligament in Postmenopausal Women with and without Pelvic Organ Prolapse

2015 ◽  
Vol 128 (23) ◽  
pp. 3191-3196 ◽  
Author(s):  
Zhi-Jing Sun ◽  
Lan Zhu ◽  
Jing-He Lang ◽  
Zhao Wang ◽  
Shuo Liang
Keyword(s):  
Pelvic Organ Prolapse ◽  
Postmenopausal Women ◽  
Proteomic Analysis ◽  
Pelvic Organ ◽  
Uterosacral Ligament

scholarly journals Atmospheric Pressure Plasma Grafting of a Vinyl-Quaternary Compound to Nonwoven Polypropylene and Cotton

2018 ◽  
Vol 13 (3) ◽  
pp. 155892501801300
Author(s):  
Carrie Cornelius ◽  
Marian McCord ◽  
Mohamed Bourham ◽  
Peter Hauser
Keyword(s):  
North Carolina ◽  
Atmospheric Pressure ◽  
Atmospheric Pressure Plasma ◽  
Atmospheric Plasma ◽  
Quaternary Compound ◽  
North Carolina State University ◽  
Pressure Plasma ◽  
Plasma Device ◽  
Plasma Grafting ◽  
Graft Yield

Nonwoven polypropylene and cotton fabrics are grafted to a vinyl quaternary compound using atmospheric-pressure plasma. Two different atmospheric plasma devices are used -the NCAPS (North Carolina Atmospheric Plasma System), a dielectric barrier discharge device created by North Carolina State University, and a plasma device from APJeT® Inc. The addition of additives such as Mohr's salt, potassium persulfate, and diacrylates are assessed to see if graft yield can be increased. Acid dye tests, SEM, and XPS reveal successful grafting of the vinyl quaternary compound. A combination of all four additives is found to yield the highest graft yields and greatest uniformity.

Proteomics of Uterosacral Ligament Connective Tissue from Women with and without Pelvic Organ Prolapse

2018 ◽  
Vol 13 (4) ◽  
pp. 1800086
Author(s):  
Xiang‐Juan Li ◽  
Hai‐Tao Pan ◽  
Juan‐Juan Chen ◽  
Yi‐Bin Fu ◽  
Min Fang ◽  
...  
Keyword(s):  
Pelvic Organ Prolapse ◽  
Connective Tissue ◽  
Pelvic Organ ◽  
Uterosacral Ligament

scholarly journals Transvaginal natural orifice transluminal endoscopic surgery for uterosacral ligament suspension: pilot study of 35 cases of severe pelvic organ prolapse

BMC Surgery ◽  
2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Zhiying Lu ◽  
Yisong Chen ◽  
Xiaojuan Wang ◽  
Junwei Li ◽  
Keqin Hua ◽  
...  
Keyword(s):  
Pelvic Organ Prolapse ◽  
Pelvic Organ ◽  
Uterosacral Ligament ◽  
Uterosacral Ligament Suspension ◽  
Efficacy And Safety ◽  
Natural Orifice ◽  
Short Term ◽  
Before And After ◽  
The Mean

Abstract Background To describe the short-term outcomes of transvaginal natural orifice transluminal endoscopic surgery (vNOTES) for uterosacral ligament suspension (USLS) in patients with severe prolapse. Methods This was a retrospective study of patients with severe prolapse (≥ stage 3) who underwent vNOTES for USLS between May 2019 and July 2020. The Pelvic Organ Prolapse Quantification (POP-Q) score, Pelvic Organ Prolapse/Urinary Incontinence Sexual Questionnaire short form (PISQ-12) and Pelvic Floor Inventory-20 (PFDI-20) were used to evaluate physical prolapse and quality of life before and after vNOTES for USLS. Results A total of 35 patients were included. The mean operative duration was 111.7 ± 39.4 min. The mean blood loss was 67.9 ± 35.8 ml. Statistically significant differences were observed between before and after vNOTES USLS in Aa (+ 0.6 ± 1.7 versus − 2.9 ± 0.2), Ba (+ 1.9 ± 2.2 versus − 2.9 ± 0.3), C (+ 1.5 ± 2.2 versus − 6.9 ± 0.9), Ap (− 1.4 ± 1.0 versus − 3.0 ± 0.1) and Bp (− 1.1 ± 1.4 versus − 2.9 ± 0.1) (P < 0.05 for all). The mean pre- and postoperative PFDI-20 score was 19.9 ± 6.7 and 3.2 ± 5.4, respectively, and the mean pre- and postoperative PISQ-12 score was 24.8 ± 2.3 and 38.3 ± 4.1, respectively (P < 0.05 for both). During 1–13 months of follow-up, there were no cases of severe complications or recurrence. Conclusions vNOTES for USLS may be a feasible technique to manage severe prolapse, with promising short-term efficacy and safety. Larger studies with more patients and longer follow-up periods should be performed to evaluate the long-term efficacy and safety profile of vNOTES for USLS.

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