scholarly journals IGF-1 regulates the growth of fibroblasts and extracellular matrix deposition in pelvic organ prolapse

Open Medicine ◽  
2020 ◽  
Vol 15 (1) ◽  
pp. 833-840
Author(s):  
Yitong Yin ◽  
Ying Han ◽  
Chang Shi ◽  
Zhijun Xia
Keyword(s):  
Pelvic Organ Prolapse ◽  
Cell Viability ◽  
Cell Apoptosis ◽  
Nuclear Factor Κb ◽  
Pelvic Organ ◽  
Vaginal Wall ◽  
Western Blot Assay ◽  
Matrix Deposition ◽  
Thiazolyl Blue Tetrazolium Bromide ◽  
The Impact

AbstractThis study was carried out to observe the impact of insulin-like growth factor-1 (IGF-1) on human vaginal fibroblasts (HVFs) in the context of pelvic organ prolapse (POP) and to explore its effects on mitogen-activated protein kinases (MAPK) and nuclear factor-κB (NF-κB) signaling pathways. First, it was found that IGF-1 expression reduced in the vaginal wall tissues derived from POP compared to that in non-POP cases. Then the role of IGF-1 was explored in HVFs and thiazolyl blue tetrazolium bromide (MTT) and flow cytometry were used to detect cell viability and cell apoptosis. Western blot assay and quantitative real-time polymerase chain reaction were used to detect the protein and mRNA expression. The results showed that knockdown of IGF-1 inhibited the cell viability of HVFs, promoted the cell apoptosis of HVFs, and decreased the expression of types I and III collagen in HVFs, which was through inhibiting the expression of IGF-1 receptor and MAPK/NF-κB pathways. However, IGF-1 plasmid had the opposite effects on HVFs. In conclusion, our results showed that IGF-1 could activate MAPK and NF-κB pathways, thereby enhancing collagen metabolism and the growth of vaginal wall fibroblasts then to inhibit POP development.

scholarly journals The Cytotoxic Effect of the Benzene Metabolite Hydroquinone is Mediated by the Modulation of MDR1 Expression via the NF-κB Signaling Pathway

2015 ◽  
Vol 37 (2) ◽  
pp. 592-602 ◽  
Author(s):  
Jianshu Huang ◽  
Mingdong Zhao ◽  
Xiuju Li ◽  
Li Ma ◽  
Jihong Zhang ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Cell Viability ◽  
Cell Apoptosis ◽  
Nuclear Translocation ◽  
Nuclear Factor Κb ◽  
Inhibitory Effect ◽  
Mdr1 Expression ◽  
Underlying Mechanisms ◽  
Cycle Regulation ◽  
The Impact

Background/Aims: Benzene is a toxic chemical whose leukemogenic effects have been studied for decades. The mechanisms of benzene-induced toxicity and leukemogenicity are not fully understood, although the involvement of several pathways has been suggested, including oxidative stress, DNA damage, cell cycle regulation and programmed cell death. In the present study, we investigated the effect of hydroquinone (HQ), a major benzene metabolite, on the viability of bone marrow derived mesenchymal stem cells (BMSCs) and explored the underlying mechanisms. Methods: First, we study the the effect of HQ on BMSCs cell viability, apoptosis and the expressions of MDR1 and NF-κB. Then we investigate the MDR1 on cell viability and cell apoptosis for BMSCs under HQ treatment. Finally, we studied the impact of nuclear factor κB (NF-κB) on the expression of MDR1. Results: Our results showed that HQ decreased cell viability and promoted cell apoptosis of BMSCs, as determined by the MTT assay and flow cytometry. Western blotting and quantitative PCR showed that HQ downregulated the expression of the MDR1 gene by inhibiting the activation and nuclear translocation of the transcription factor NF-κB. Overexpression of MDR1 attenuated the inhibitory effect of HQ on cell viability in BMSC. Conclusion: The results of the present study suggest the involvement of the multidrug resistance membrane transporter MDR1 and the NF-κB pathway in the cytotoxicity of benzene and its metabolites. Further studies are necessary to clarify the role of the pathways involved and the crosstalk between them in mediating the effects of HQ in bone marrow progenitor cells.

scholarly journals Improvement of Cell Growth of Uterosacral Ligament Fibroblast Derived from Pelvic Organ Prolapse Patients by Cold Atmospheric Plasma Treated Liquid

Cells ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 2728
Author(s):  
Ihn Han ◽  
Seung Ah Choi ◽  
Seul I Kim ◽  
Eun Ha Choi ◽  
Young Joo Lee ◽  
...  
Keyword(s):  
Pelvic Organ Prolapse ◽  
Cell Viability ◽  
Cell Apoptosis ◽  
Atmospheric Pressure Plasma ◽  
Pelvic Organ ◽  
Uterosacral Ligament ◽  
Atmospheric Plasma ◽  
Skov3 Cells ◽  
Plasma Device ◽  
Barrier Discharge Plasma

Pelvic organ prolapse (POP) is a chronic disorder that affects quality of life in women. Several POP treatments may be accompanied by abrasion, constant infection, and severe pain. Therefore, new treatment methods and improvements in current treatments for POP are required. Non-thermal atmospheric-pressure plasma is a rising biomedical therapy that generates a mixed cocktail of reactive species by different mechanisms. In this study, we applied a cylinder-type dielectric barrier discharge plasma device to create a plasma-treated liquid (PTL). The PTL was added to primary cultured human uterosacral ligament fibroblast (hUSLF) cells from POP patients at each stage. Surprisingly, treatment with diluted PTL increased hUSLF cell viability but decreased ovarian cancer cell viability. PTL also decreased cell apoptosis in hUSLF cells but increased it in SKOV3 cells. Our results suggest that PTL protects hUSLF cells from cell apoptosis by controlling the p53 pathway and improves cell viability, implying that PTL is a promising application for POP therapy.

scholarly journals Sexual function in women with pelvic floor disorders

2013 ◽  
Vol 7 (9-10) ◽  
pp. 199 ◽  
Author(s):  
Rebecca G. Rogers
Keyword(s):  
Urinary Incontinence ◽  
Pelvic Organ Prolapse ◽  
Stress Urinary Incontinence ◽  
Sexual Function ◽  
Pelvic Floor ◽  
Pelvic Floor Disorders ◽  
Pelvic Floor Dysfunction ◽  
Pelvic Organ ◽  
The Impact

Pelvic floor disorders (PFDs) can impact sexual function. This summary provides an overview of the impact of stress urinary incontinence and pelvic organ prolapse and their treatments on sexual function. In general, interventions that successfully address PFDs will generally improve sexual function as well. However, there are patients whose sexual function will remain unchanged despite treatment, and a small but significant minority who will report worsened sexual function following treatment for their pelvic floor dysfunction.

Tissue Composition of the Vaginal Wall in Women with Pelvic Organ Prolapse

2013 ◽  
Vol 75 (1) ◽  
pp. 21-27 ◽  
Author(s):  
Aukje M. Meijerink ◽  
Reinier H. van Rijssel ◽  
Paul J.Q. van der Linden
Keyword(s):  
Pelvic Organ Prolapse ◽  
Pelvic Organ ◽  
Vaginal Wall ◽  
Tissue Composition

scholarly journals Changes in muscularis propria of anterior vaginal wall in women with pelvic organ prolapse

2016 ◽  
Vol 60 (1) ◽  
Author(s):  
A. Vetuschi ◽  
A. D'Alfonso ◽  
R. Sferra ◽  
D. Zanelli ◽  
S. Pompili ◽  
...  
Keyword(s):  
Risk Factors ◽  
Smooth Muscle ◽  
Pelvic Organ Prolapse ◽  
Smooth Muscle Cells ◽  
Muscularis Propria ◽  
Muscle Cells ◽  
Pelvic Organ ◽  
Vaginal Wall ◽  
Anterior Vaginal Wall ◽  
Collagen Iii

<p>The objective<strong> </strong>of this study was to evaluate the morphological and immunohistochemical alterations of tissue removed from the upper third of anterior vaginal wall in a sample group of the female population presenting homogenous risk factors associated with Pelvic Organ Prolapse (POP). The case study consisted of 14 patients with POP and there were 10 patients in the control group. Patient selection was carried on the basis of specific criteria and all of the patients involved in the study presented one or more of the recognized POP risk factors. Samples were taken from POP patients during vaginal plastic surgery following  colpohysterectomy, and from control patients during closure of the posterior fornix following hysterectomy. Samples were processed for histological and  immunohistochemical analyses for Collagen I and Collagen III, α-Smooth Muscle Actin (α-SMA), Platelet-Derived-Growth-Factor (PDGF), matrix metalloproteinase 3 (MMP3), Caspase3. Immunofluorescence analyses for Collagen I and III and PDGF were also carried out. In prolapsed specimens our results show a disorganization of smooth muscle cells that appeared to have been displaced by an increased collagen III deposition resulting in rearrangement of the muscularis propria architecture. These findings suggest that the increase in the expression of collagen fibers in muscularis could probably due to a phenotypic switch resulting in the dedifferentiation of smooth muscle cells into myofibroblasts. These alterations could be responsible for the compromising of the dynamic functionality of the pelvic floor.</p><p><strong> </strong></p>

Detection of oncological diseases in patients with pelvic organ prolapse. Case report

GYNECOLOGY ◽  
2021 ◽  
Vol 23 (2) ◽  
pp. 193-197
Author(s):  
Dmitrii V. Baibuz ◽  
Oleg L. Molchanov ◽  
Zhanna V. Glushchenko ◽  
Yanina A. Lebedeva ◽  
Sergei V. Utkin ◽  
...  
Keyword(s):  
Pelvic Organ Prolapse ◽  
Preoperative Diagnosis ◽  
Abdominal Cavity ◽  
Pelvic Organ ◽  
Vaginal Wall ◽  
Benign Disease ◽  
Pelvic Tumor ◽  
Planning Treatment ◽  
Oncological Diseases ◽  
Anterior Posterior

Pelvic organ prolapse is a common benign disease among women that is characterized by an isolated or combined descent of the anterior, posterior vaginal wall, uterus, and vaginal dome after hysterectomy. Given the fact that most of the operations are performed among middle-aged and elderly patients, the probability of detecting previously undiagnosed tumor of the abdominal cavity and pelvic region increases, which may be due to the limited volume of basic preoperative diagnosis. The purpose of our report is to draw the attention of clinicians to the problem of insufficient examination of women when planning treatment of pelvic organ prolapse on the example of our experience in managing a patient with this pathology in combination with pelvic tumor.

Is pelvic organ prolapse associated with altered microcirculation of the vaginal wall?

2015 ◽  
Vol 35 (7) ◽  
pp. 764-770 ◽  
Author(s):  
M.A. Weber ◽  
D.M.J. Milstein ◽  
C. Ince ◽  
J.P.W.R. Roovers
Keyword(s):  
Pelvic Organ Prolapse ◽  
Pelvic Organ ◽  
Vaginal Wall

Computational Modeling of Anterior and Posterior Pelvic Organ Prolapse (POP)

2016 ◽  
Author(s):  
Arnab Chanda ◽  
Vinu Unnikrishnan ◽  
Holly E. Richter ◽  
Mark E. Lockhart
Keyword(s):  
Pelvic Organ Prolapse ◽  
Urinary Bladder ◽  
High Stress ◽  
Significant Proportion ◽  
Pelvic Organ ◽  
Vaginal Wall ◽  
Vaginal Tissue ◽  
Bladder Filling ◽  
Vaginal Length ◽  
Vaginal Canal

Pelvic Organ Prolapse (POP) is a condition of the female pelvic system suffered by a significant proportion of women in the U.S. and more across the globe, every year. POP is caused by the weakening of the pelvic floor muscles and musculo-connective tissues due to child birth, menopause and morbid obesity. Prolapse of the pelvic organs namely the urinary bladder, uterus, and rectum into the vaginal canal can cause vaginal discomfort, strained urination or defecation, and sexual dysfunction. To date, success rates of native tissue POP surgeries vary from 50–70% depending on the definition of cure and time-point of assessment. A better understanding of the mechanics of prolapse may lead to improvement in surgical outcomes. In the current work, the mechanics of progression of anterior and posterior vaginal prolapse were modeled to understand the effect of bladder fill and posterior vaginal stresses using computational approaches. A realistic and full-scale female pelvic system model, comprised of the urinary bladder, vaginal canal, uterus, rectum, and fascial connective tissue, was developed using image segmentation methods. All of the relevant loads and boundary conditions were applied based on a comprehensive study of the anatomy and functional morphology of the female pelvis. Hyperelastic material models were adopted to characterize all pelvic tissues, and a non-linear analysis was invoked. In the first set of simulations, a realistic bladder filling and vaginal tissue stiffening in prolapse were modeled and their effects on the anterior vaginal wall (AVW) were estimated in terms of the induced stresses, strains and displacements. The degree of bladder filling was found to be a strong indicator of stress build-up on the AVW. Also, vaginal tissue stiffening was found to increase the size of the high stress zone on the AVW. The second simulation consisted of modeling the different degrees of posterior vaginal wall (PVW) prolapse, in the presence of an average abdominal pressure. The vaginal length was segmented into four sections to study the localized stresses and strains. Also, a clinically well-known phenomena known as the kneeling effect was observed with the PVW in which the vaginal wall displaces away from the rectum and downward towards the vaginal hiatus. All of these results have relevant clinical implications and may provide important perspective for better understanding the mechanics of POP pathophysiology.

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