scholarly journals Transcriptomic Insights into Mechanisms of Early Seed Maturation in the Garden Pea (Pisum sativum L.)

Cells ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 779 ◽  
Author(s):  
Yury V. Malovichko ◽  
Oksana Y. Shtark ◽  
Ekaterina N. Vasileva ◽  
Anton A. Nizhnikov ◽  
Kirill S. Antonets
Keyword(s):  
Pisum Sativum ◽  
Hormonal Regulation ◽  
Dna Methyltransferase ◽  
De Novo ◽  
Seed Maturation ◽  
Garden Pea ◽  
Altered Expression ◽  
Early Maturation ◽  
Pisum Sativum L ◽  
Maturation Rate

The garden pea (Pisum sativum L.) is a legume crop of immense economic value. Extensive breeding has led to the emergence of numerous pea varieties, of which some are distinguished by accelerated development in various stages of ontogenesis. One such trait is rapid seed maturation, which, despite novel insights into the genetic control of seed development in legumes, remains poorly studied. This article presents an attempt to dissect mechanisms of early maturation in the pea line Sprint-2 by means of whole transcriptome RNA sequencing in two developmental stages. By using a de novo assembly approach, we have obtained a reference transcriptome of 25,756 non-redundant entries expressed in pea seeds at either 10 or 20 days after pollination. Differential expression in Sprint-2 seeds has affected 13,056 transcripts. A comparison of the two pea lines with a common maturation rate demonstrates that while at 10 days after pollination, Sprint-2 seeds show development retardation linked to intensive photosynthesis, morphogenesis, and cell division, and those at 20 days show a rapid onset of desiccation marked by the cessation of translation and cell anabolism and accumulation of dehydration-protective and -storage moieties. Further inspection of certain transcript functional categories, including the chromatin constituent, transcription regulation, protein turnover, and hormonal regulation, has revealed transcriptomic trends unique to specific stages and cultivars. Among other remarkable features, Sprint-2 demonstrated an enhanced expression of transposable element-associated open reading frames and an altered expression of major maturation regulators and DNA methyltransferase genes. To the best of our knowledge, this is the first comparative transcriptomic study in which the issue of the seed maturation rate is addressed.

Breeding Strategies of Garden Pea (Pisum sativum L.)

2021 ◽  
pp. 331-377
Author(s):  
Amal M. E. Abdel-Hamid ◽  
Khaled F. M. Salem
Keyword(s):  
Pisum Sativum ◽  
Breeding Strategies ◽  
Garden Pea ◽  
Pisum Sativum L

scholarly journals Introgression of the Afila Gene into Climbing Garden Pea (Pisum sativum L.)

Agronomy ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 1537
Author(s):  
Oscar Checa ◽  
Marino Rodriguez ◽  
Xingbo Wu ◽  
Matthew Blair
Keyword(s):  
Pisum Sativum ◽  
Production Costs ◽  
Yield Potential ◽  
Recurrent Parent ◽  
Linkage Drag ◽  
Progeny Testing ◽  
High Biomass ◽  
Garden Pea ◽  
Pisum Sativum L ◽  
Field Peas

The pea (Pisum sativum L.) is one of the most important crops in temperate agriculture around the world. In the tropics, highland production is also common with multiple harvests of nearly mature seeds from climbing plant types on trellises. While the leafless variant caused by the afila gene is widely used in developing row-cropped field peas in Europe, its use for trellised garden peas has not been reported. In this study we describe a pea breeding program for a high-elevation tropical environment in the Department of Nariño in Colombia, where over 16,000 hectares of the crop are produced. The most widespread climbing varieties in the region are ‘Andina’ and ‘Sindamanoy’, both of which have high-biomass architecture with abundant foliage. They are prone to many diseases, but preferred by farmers given their long production season. This plant type is expensive to trellis, with wooden posts and plastic strings used for vine staking constituting 52% of production costs. The afila trait could reduce these costs by creating interlocking plants as they do in field peas. Therefore, our goal for this research was to develop a rapid breeding method to introduce the recessive afila gene, which replaces leaves with tendrils, into the two commercial varieties used as recurrent parents (RPs) with three donor parents (DPs)—‘Dove’, ‘ILS3575′ and ‘ILS3568′—and to measure the effect on plant height (PH) and yield potential. Our hypothesis was that the afila gene would not cause linkage drag while obtaining a leafless climbing pea variety. Backcrossing was conducted without selfing for two generations and plants were selected to recover recurrent parent characteristics. Chi-square tests showed a ratio of 15 normal leaved to one afila leaved in the BC2F2 plants, and 31:1 in the BC3F2 generation. Selecting in the last of these generations permitted a discovery of tall climbing plants that were similar to those preferred commercially, but with the stable leafless afila. The method saved two seasons compared to the traditional method of progeny testing before each backcross cycle; the peas reached the BC2F2 generation in five seasons and the BC3F2 in seven seasons. This is advantageous with trellised peas that normally require half a year to reach maturity. Leafless garden peas containing the afila gene were of the same height as recurrent parents and, by the third backcross, were equally productive, without the high biomass found in the traditional donor varieties. The value of the afila gene and the direct backcrossing scheme is discussed in terms of garden pea improvement and crop breeding.

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