scholarly journals Methodology of Selecting the Optimal Receptor to Create an Electrochemical Immunosensor for Equine Arteritis Virus Protein Detection

Chemosensors ◽  
2021 ◽  
Vol 9 (9) ◽  
pp. 265
Author(s):  
Mateusz Brodowski ◽  
Marcin Kowalski ◽  
Wioleta Białobrzeska ◽  
Katarzyna Pałka ◽  
Rafał Walkusz ◽  
...  
Keyword(s):  
Electrochemical Impedance ◽  
Protein Detection ◽  
Electrochemical Immunosensor ◽  
Equine Arteritis Virus ◽  
Rapid Screening ◽  
Virus Protein ◽  
Glycoprotein G ◽  
Streptococcus Equi ◽  
Rapid Screening Test ◽  
High Concentrations

The study reports a methodology of selecting the optimal receptor to create an electrochemical immunosensor for equine arteritis virus (EAV) protein detection. The detection was based on antigen recognition by antibodies immobilized on gold electrodes. Modification steps were controlled by electrochemical impedance spectroscopy and cyclic voltammetry measurements. In order to obtain the impedance immunosensor with the best parameters, seven different receptors complementary to equine arteritis virus protein were used. In order to make the selection, a rapid screening test was carried out to check the sensor’s response to blank, extremely low and high concentrations of target EAV protein, and negative sample: M protein from Streptococcus equi and glycoprotein G from Equid alphaherpesvirus 1. F6 10G receptor showed the best performance.

Rapid screening test for porphyrinuria

1970 ◽  
Vol 102 (2) ◽  
pp. 237-237
Author(s):  
R. M. McDonald
Keyword(s):  
Screening Test ◽  
Rapid Screening ◽  
Rapid Screening Test

A Rapid Screening Test for Detection of Poisons in Blood

Nature ◽  
1962 ◽  
Vol 193 (4822) ◽  
pp. 1298-1298 ◽  
Author(s):  
H. V. STREET
Keyword(s):  
Screening Test ◽  
Rapid Screening ◽  
Rapid Screening Test

scholarly journals Evaluation of a prototype sub-unit vaccine against equine arteritis virus comprising the entire ectodomain of the virus large envelope glycoprotein (GL): induction of virus-neutralizing antibody and assessment of protection in ponies

2001 ◽  
Vol 82 (10) ◽  
pp. 2425-2435 ◽  
Author(s):  
J. Castillo-Olivares ◽  
A. A. F. de Vries ◽  
M. J. B. Raamsman ◽  
P. J. M. Rottier ◽  
K. Lakhani ◽  
...  
Keyword(s):  
Envelope Glycoprotein ◽  
Neutralizing Antibody ◽  
Equine Arteritis Virus ◽  
Post Vaccination ◽  
Degree Of Protection ◽  
Glycoprotein G ◽  
Virus Excretion ◽  
Unvaccinated Control ◽  
Antibody Titres ◽  
Immunization Study

An Escherichia coli-expressed recombinant protein (6hisGLecto) comprising the entire ectodomain (aa 18–122) of equine arteritis virus (EAV) glycoprotein GL, the immunodominant viral antigen, induced higher neutralizing antibody titres than other GL-derived polypeptides when compared in an immunization study in ponies. The potential of the recombinant GL ectodomain to act as a sub-unit vaccine against EAV was evaluated further in three groups of four ponies vaccinated with doses of 35, 70 or 140 μg of protein. All vaccinated animals developed a virus-neutralizing antibody (VNAb) response with peak titres 1–2 weeks after the administration of a booster on week 5 (VNAb titres of 1·8–3·1), 13 (VNAb titres of 1·4–2·9) or 53 (VNAb titres of 1·2–2·3). Vaccinated and unvaccinated control ponies were infected with EAV at different times post-vaccination to obtain information about the degree of protection relative to the levels of pre-challenge VNAb. Vaccination conferred varying levels of protection, as indicated by reduced or absent pyrexia, viraemia and virus excretion from the nasopharynx. The degree of protection correlated well with the levels of pre-challenge VNAb and, in particular, with levels of virus excretion. These results provide the first evidence that a sub-unit vaccine protects horses against EAV. The use of the sub-unit vaccine in combination with a differential diagnostic test based on other EAV antigens would enable serological discrimination between naturally infected and vaccinated equines.

Erythrocyte adenosine transport a rapid screening test for cardiovascular drugs

1993 ◽  
Vol 30 (3) ◽  
pp. 163-167 ◽  
Author(s):  
Pollen K.F. Yeung ◽  
Susan J. Mosher ◽  
Rongshi Li ◽  
Patrick S. Farmer ◽  
Gerald A. Klassen ◽  
...  
Keyword(s):  
Screening Test ◽  
Cardiovascular Drugs ◽  
Rapid Screening ◽  
Rapid Screening Test ◽  
Adenosine Transport

scholarly journals On the effects of some carcinogenic hydrocarbons on the growth of sporelings of marine red algae

1962 ◽  
Vol 42 (3) ◽  
pp. 579-585 ◽  
Author(s):  
A. D. Boney ◽  
E. D. S. Corner
Keyword(s):  
Aromatic Hydrocarbons ◽  
Red Algae ◽  
Considerable Increase ◽  
Rapid Screening ◽  
Cell Production ◽  
Low Concentrations ◽  
Rapid Screening Test ◽  
Polycyclic Aromatic ◽  
Carcinogenic Hydrocarbons ◽  
Derivatives Of

Low concentrations of various carcinogenic polycyclic aromatic hydrocarbons cause a considerable increase in cell production when applied to sporelings of certain marine red algae.Whereas low concentrations of carcinogenic derivatives of benzanthracene stimulate cell production, similar concentrations of structurally related non-carcinogens inhibit it.The applicability of the method as a rapid screening test for carcinogens is discussed.

Electrochemical Immunosensor Based on Highly Sensitive Amino Functionalized Graphene Nanoplatelets-Modified Screen Printed Carbon Electrode

2020 ◽  
Vol 833 ◽  
pp. 171-175
Author(s):  
Nurul Azurin Badruzaman ◽  
Mohd Azraie Mohd Azmi ◽  
Nur Azura Mohd Said
Keyword(s):  
Incubation Time ◽  
Electrochemical Impedance ◽  
Electrochemical Immunosensor ◽  
Graphene Nanoplatelets ◽  
Carbon Electrode ◽  
Functionalized Graphene ◽  
Working Electrode ◽  
Screen Printed Carbon Electrode ◽  
Rabbit Igg ◽  
Screen Printed

We presented here the development of an immunosensor based on graphene nanoplatelets-modified screen printed carbon electrode (SPCE) with incorporated rabbit IgG on the amino functionalized surface area. In order to improve sensitivity of working electrode, graphene-nanoplatelets solution was fabricated onto surface carbon working electrode. The effect of different (3-aminopropyl) triethoxysilane (APTES) concentrations (0.125, 0.5, 2 and 8% (v/v)) and incubation time of silanization (30, 60 and 90 min) were studied and compared. An electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) were used to characterize our immunosensor based. It is showed that the optimum APTES concentration which provides higher surface coverage and electron transfer rate was 2% concentration (v/v) at 60 min of incubation time. The modified surface was then evaluated by measuring immobilized rabbit IgG via indirect assay using horseradish peroxidase labelled secondary antibody. The optimum detection immobilized IgG was 0.05 mg/mL. These results indicate the potential for amino functionalized graphene nanoplatelets-modified SPCE in detecting protein biomarkers.

Rapid Solubility Test for Detection of Hemoglobin S

1971 ◽  
Vol 17 (11) ◽  
pp. 1081-1082 ◽  
Author(s):  
Jean E Matusik ◽  
James B Powell ◽  
David M Gregory
Keyword(s):  
Phosphate Buffer ◽  
Screening Test ◽  
Rapid Screening ◽  
Hemoglobin S ◽  
Solubility Test ◽  
Negative Results ◽  
Rapid Screening Test

Abstract We have devised a rapid screening test for use in detecting sickling hemoglobins. This test depends on the insolubility of sickling hemoglobins in 2.24 molar phosphate buffer. Sickling hemoglobins form a turbid suspension in this test, in which positive and negative results, respectively, appear very much like those for the "Sickledex" test.

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