scholarly journals Ionic Liquids as Protein Crystallization Additives

Crystals ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 1166
Author(s):  
Crissy L. Tarver ◽  
Qunying Yuan ◽  
Marc L. Pusey
Keyword(s):  
Ionic Liquids ◽  
Pathogenic Bacteria ◽  
Alkyl Chain ◽  
Protein Crystallization ◽  
Dihydrogen Phosphate ◽  
Base Metals ◽  
Butyl Group ◽  
Crystallization Conditions

Among its attributes, the mythical philosopher’s stone is supposedly capable of turning base metals to gold or silver. In an analogous fashion, we are finding that protein crystallization optimization using ionic liquids (ILs) often results in the conversion of base protein precipitate to crystals. Recombinant inorganic pyrophosphatases (8 of the 11 proteins) from pathogenic bacteria as well as several other proteins were tested for optimization by 23 ILs, plus a dH2O control, at IL concentrations of 0.1, 0.2, and 0.4 M. The ILs were used as additives, and all proteins were crystallized in the presence of at least one IL. For 9 of the 11 proteins, precipitation conditions were converted to crystals with at least one IL. The ILs could be ranked in order of effectiveness, and it was found that ~83% of the precipitation-derived crystallization conditions could be obtained with a suite of just eight ILs, with the top two ILs accounting for ~50% of the hits. Structural trends were found in the effectiveness of the ILs, with shorter-alkyl-chain ILs being more effective. The two top ILs, accounting for ~50% of the unique crystallization results, were choline dihydrogen phosphate and 1-butyl-3-methylimidazolium tetrafluoroborate. Curiously, however, a butyl group was present on the cation of four of the top eight ILs.

Spectroscopic Study on Imidazolium-Based Ionic Liquids: Effect of Alkyl Chain Length and Anion

2012 ◽  
Vol 116 (11) ◽  
pp. 3512-3518 ◽  
Author(s):  
Enrico Binetti ◽  
Annamaria Panniello ◽  
Leonardo Triggiani ◽  
Raffaele Tommasi ◽  
Angela Agostiano ◽  
...  
Keyword(s):  
Ionic Liquids ◽  
Spectroscopic Study ◽  
Chain Length ◽  
Alkyl Chain ◽  
Alkyl Chain Length

Relationship between mesoscale dynamics and shear relaxation of ionic liquids with long alkyl chain

2012 ◽  
Vol 137 (10) ◽  
pp. 104511 ◽  
Author(s):  
Tsuyoshi Yamaguchi ◽  
Ken-ichi Mikawa ◽  
Shinobu Koda ◽  
Kenta Fujii ◽  
Hitoshi Endo ◽  
...  
Keyword(s):  
Ionic Liquids ◽  
Alkyl Chain ◽  
Mesoscale Dynamics ◽  
Shear Relaxation ◽  
Long Alkyl Chain

Aggregation Behaviours of Surface-Active Ionic Liquids 1-Alkyl-3-methylimidazolium Bis(2-ethylhexyl)sulfosuccinate

2016 ◽  
Vol 69 (11) ◽  
pp. 1254 ◽  
Author(s):  
Jiequn Wu ◽  
Tianxiang Yin ◽  
Shaoxiong Shi ◽  
Weiguo Shen
Keyword(s):  
Ionic Liquids ◽  
Self Assembly ◽  
Alkyl Chain ◽  
Systematic Investigation ◽  
Bulk Solution ◽  
Free Energies ◽  
Imidazolium Cation ◽  
Structure Transition ◽  
Surface Active ◽  
Counter Ion Binding

The systematic investigation of the aggregation behaviours of newly synthesised surface-active ionic liquids 1-alkyl-3-methylimidazolium bis(2-ethylhexyl)sulfosuccinate ([Cnmim][AOT], n = 2, 3, 5, 6, 7) by various techniques is reported. The critical aggregation concentrations (CACs) and the standard Gibbs free energies of aggregation () were determined from measurements on conductivity, fluorescence, and surface tension, which suggested a stronger self-assembly ability in the bulk solution for [Cnmim][AOT] surfactants with longer alkyl chain cations. An interesting structure transition driven by the penetration of the imidazolium cation into the aggregate when n > 4 was found by analysis of the variations of the values of CAC, , the degree of counter ion binding (β), and the micropolarity (I1/I3) immediately after the CAC with changing alkyl chain length of the imidazolium cation, which was further confirmed by 1H NMR measurements.

Long alkyl chain quaternary ammonium-based ionic liquids and potential applications

2006 ◽  
Vol 8 (9) ◽  
pp. 798 ◽  
Author(s):  
Juliusz Pernak ◽  
Marcin Smiglak ◽  
Scott T. Griffin ◽  
Whitney L. Hough ◽  
Timothy B. Wilson ◽  
...  
Keyword(s):  
Ionic Liquids ◽  
Quaternary Ammonium ◽  
Alkyl Chain ◽  
Potential Applications ◽  
Long Alkyl Chain

Thermo-physical and tribological characteristics of ionic liquid-based rice bran oil as green cutting fluid

2021 ◽  
pp. 135065012110461
Author(s):  
Aswani K Singh ◽  
Varun Sharma
Keyword(s):  
Ionic Liquid ◽  
Ionic Liquids ◽  
Coefficient Of Friction ◽  
Rice Bran ◽  
Alkyl Chain ◽  
Rice Bran Oil ◽  
Alkyl Chain Length ◽  
Cutting Fluid ◽  
Cutting Fluids ◽  
Base Oil

During machining, the cutting fluids play an essential role in cooling and lubrication. In order to reduce the friction forces, the excessive amount of the cutting fluids are generally used. This, in turn, leads to wastage of the cutting fluids which results in a serious impact on the environment, health and cost of production. Therefore, the judicious use of lubricants is the foremost concern in the manufacturing industry. In order to mitigate these drawbacks, various alternatives have been developed in the last decade. In the present paper, ionic liquids have been proved as favourable sustainable alternative additives in the base oil. The effect of alkyl chain length of ionic liquids with base oil on the thermo-physical and tribological characteristics of cutting fluids including viscosity, wettability, anticorrosion behaviour, thermal stability, and coefficient of friction have been analysed. In the present study, pyrrolidinium and hexafluoro-phosphate (PF6) have been used as cation and anion, respectively, with rice bran oil as base oil. The five different ionic liquids have been dispersed in base oil by 1.0 wt%. It has been found that longer alkyl chain length showed the favourable results as compared to the shorter one. Results indicated that ionic liquid based cutting fluid attained ample enhanced thermophysical and tribological properties as compared to the neat rice bran oil. There has been 5.08% and 4.29% improvement in viscosity and thermal conductivity for IL4 + RBO in comparison to neat RBO. In addition, the wettability, coefficient of friction, and wear volume have been reduced by 20.34%, 53.79% and 57.87% correspondingly.

Temperature-Screening System for Determining Protein Crystallization Conditions

2005 ◽  
Vol 44 (6A) ◽  
pp. 4080-4083 ◽  
Author(s):  
Hiroaki Adachi ◽  
Ai Niino ◽  
Kazufumi Takano ◽  
Hiroyoshi Matsumura ◽  
Satoshi Murakami ◽  
...  
Keyword(s):  
Protein Crystallization ◽  
Screening System ◽  
Crystallization Conditions

scholarly journals Characterization and Functionality of Immidazolium Ionic Liquids Modified Magnetic Nanoparticles

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Ying Li ◽  
Ning Tang ◽  
Fuyuhiko Inagaki ◽  
Chisato Mukai ◽  
Kazuichi Hayakawa
Keyword(s):  
Ionic Liquids ◽  
Magnetic Nanoparticles ◽  
Extraction Efficiency ◽  
Alkyl Chain ◽  
Organic Pollutant ◽  
High Adsorption ◽  
Transmission Electron ◽  
Polycyclic Aromatic ◽  
One Step ◽  
Modified Magnetic Nanoparticles

1,3-Dialkylimidazolium-based ionic liquids were chemically synthesized and bonded on the surface of magnetic nanoparticles (MNPs) with easy one-step reaction. The obtained six kinds of ionic liquid modified MNPs were characterized with transmission electron microscopy, thermogravimetric analysis, magnetization, and FTIR, which owned the high adsorption capacity due to the nanometer size and high-density modification with ionic liquids. Functionality of MNPs with ionic liquids greatly influenced the solubility of the MNPs with organic solvents depending on the alkyl chain length and the anions of the ionic liquids. Moreover, the obtained MNPs showed the specific extraction efficiency to organic pollutant, polycyclic aromatic hydrocarbons, while superparamagnetic property of the MNPs facilitated the convenient separation of MNPs from the bulks water samples.

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