Morphological and Molecular Characterization of Trichuris sp. (Nematoda: Trichuridae) in Crested Porcupines (Hystrix cristata; Rodentia: Hystricidae) from Italy

Adult specimens of Trichuris sp. collected from crested porcupines (Hystrix cristata) from Italy were characterized using an integrative taxonomic approach involving morphological and molecular tools. The morphological features of this Trichuris sp. were compared to data already available for Trichuris spp. from Hystrix sp., revealing diagnostic traits, such as spicule length in males or vulva shape in females, which distinguish this Trichuris sp. from the other species. Evidence from sequences analysis of the partial mitochondrial COX1 region indicated that the taxon under study is a distinct lineage. Biometrical and genetic data suggested this Trichuris sp. to be a valid and separated taxon. However, since molecular data from other Trichuris spp. infecting Hystrix, such as T. infundibulus, T. hystricis, T. javanica, T.landak and T. lenkorani, are missing in public repositories, the number and identity of distinct lineages able to infect porcupines remain only partially defined.

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Morphological and Molecular Characterization of Entomophthorales (Entomophthoromycota: Entomophthoromycotina) from Argentina

Acta Mycologica ◽

10.5586/am.5522 ◽

2020 ◽

Vol 55 (2) ◽

Author(s):

Romina G. Manfrino ◽

Louela A. Castrillo ◽

Claudia C. López Lastra ◽

Andrea V. Toledo ◽

Walter Ferrari ◽

...

Keyword(s):

Molecular Data ◽

Molecular Techniques ◽

Accurate Identification ◽

Fungal Isolates ◽

Unidentified Species ◽

First Time ◽

Aphid Host ◽

Morphological And Molecular Characterization ◽

Molecular Characters

We characterized 17 insect-pathogenic entomophthoralean fungal isolates (Entomophthoromycotina: Entomophthorales) using morphological and molecular techniques. We identified four species from various insect hosts: (i) Entomophthora planchoniana, six specimens from aphids; (ii) Pandora neoaphidis, three specimens from aphids; (iii) Zoophthora phalloides from an aphid; and (iv) Z. radicans, seven specimens from insects in the orders Diptera, Hemiptera, and Lepidoptera. Analysis of ITS1 data from E. planchoniana showed clustering in accordance to aphid host species. Entomophthora planchoniana from Macrosiphum euphorbiae clustered together, separate from the isolate from Myzus persicae. The P. neoaphidis specimens clustered with sequences from other aphid-pathogenic Pandora species in GenBank. In this study, Z. phalloides from Brevicoryne brassicae and Z. radicans from an unidentified species of Chironomidae (Diptera) in Argentina were characterized for the first time. The present study was initiated to elucidate the taxonomy of the entomophthoralean fungi in Argentina according to their morphological and molecular characters. The presented results emphasize the significance of the combination of molecular data and information on morphology, ecology, and host range for accurate identification of entomophthoralean and allied genera.

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First assessment of the diversity of coralline species forming maerl and rhodoliths in Guadeloupe, Caribbean using an integrative systematic approach

Phytotaxa ◽

10.11646/phytotaxa.190.1.13 ◽

2014 ◽

Vol 190 (1) ◽

pp. 190 ◽

Cited By ~ 11

Author(s):

Viviana Peña Freire ◽

Florence Rousseau ◽

Bruno De Reviers ◽

Line Le Gall

Keyword(s):

Systematic Approach ◽

Molecular Data ◽

Coralline Algae ◽

Anatomical Features ◽

Anatomical Data ◽

Distinct Lineage ◽

Reproductive Material ◽

The Caribbean ◽

Taxonomic Approach ◽

Study Species

The present study documents species of coralline algae that form maerl and rhodoliths in Guadeloupe, Caribbean using an integrative systematic approach of combining molecular (COI-5P, psbA) and morphological/anatomical data. Maerl and rhodoliths were collected by SCUBA and dredging from six localities in Guadeloupe during the Karubenthos Expedition, which was coordinated by the Parc National de la Guadeloupe and the Muséum National d´Histoire Naturelle. Of the twelve maerl and rhodolith specimens collected and sequenced, eight specific entities were delimitated based on the analysis of molecular data: Lithothamnion cf. ruptile, five species of the genus Lithothamnion, one species of the genus Spongites, and the remaining one was either assigned to the genus Lithoporella or Mastophora. Morphological/anatomical data are presented for each of these species. Molecular analyses revealed that Lithothamnion. cf ruptile and other four species (Lithothamnion spp.1–4) were resolved in a distinct lineage than the other Lithothamnion species examined. Nevertheless, these five species were provisionally remained in the genus Lithothamnion based on the presence of flared epithallial cells. Previously, only three species of Lithothamnion have been cited in the Caribbean. Till this study species belonging to the genus Spongites have not been hitherto reported for this region. The specimen identified as either Lithoporella or Mastophora shared anatomical features with both genera. Further examination of reproductive material is required to identify this specimen to a lower taxonomical rank. This study is the first to report the occurrence of maerl and rhodoliths belonging to the genus Lithothamnion at 110 m depth; this record being the deepest for the genus in the Caribbean Sea. Our results have revealed that species diversity of Caribbean maerl has been underestimated and further surveys coupled with an integrative taxonomic approach on this biodiversity hotspot are necessary.

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Morphological and molecular characterization of Xiphinema species from Shenzhen, China

Helminthologia ◽

10.1515/helmin-2015-0068 ◽

2016 ◽

Vol 53 (1) ◽

pp. 62-75 ◽

Cited By ~ 4

Author(s):

Y. Zeng ◽

W. Ye ◽

Z. Zhang ◽

H. Sun ◽

L. Yong ◽

...

Keyword(s):

Acacia Mangium ◽

Distinct Species ◽

Morphological Characters ◽

Molecular Data ◽

Biodiversity Survey ◽

Close Relationship ◽

Nematode Biodiversity ◽

Morphological And Molecular Characterization ◽

Partial 18S

Summary During a nematode biodiversity survey from 2012 to 2014 in Shenzhen, China, ten nematode populations (SZX1301–SZX1310) of Xiphinema were recovered from rhizosphere of different plants, namely Acacia mangium (SZX1306), A. confuse (SZX1309), Blechnum orientale (SZX1301, SZX1302, SZX1307, SZX1308), Litchi chinensis (SZX1304, SZX1310) in Tianxinshan and Gleichenia linearis (SZX1303, SZX1305) in Yangmeikeng environmental monitoring sites. Morphological and molecular profiles of these populations were determined. Three species of Xiphinema, i.e., X. hunaniense Wang & Wu, 1992, X. brasiliense Lordello, 1951 and X. americanum Cobb, 1913 sensu lato were identified using morphological characters and molecular data of partial 18S and 28S D2–D3 rDNA expansion segments. Four populations (SZX1301–SZX1304) were X. hunaniense, one population (SZX1305) X. brasiliense, and five populations (SZX1306–SZX1310) X. americanum s.l.. Phylogenetic analysis based on sequences of the 28S rDNA D2–D3 expansion segment revealed these three species are all distinct species and supported a close relationship with their corresponding species. This is the first report of X. hunaniense, X. brasiliense and X. americanum s.l. in their hosts except for L. chinensis.

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Morphological and molecular characterization of three new Parastenocarididae (Copepoda: Harpacticoida) from caves in Southern Italy

European Journal of Taxonomy ◽

10.5852/ejt.2020.689 ◽

2020 ◽

Author(s):

Maria Cristina Bruno ◽

Vezio Cottarelli ◽

Federico Marrone ◽

Rosario Grasso ◽

Erika Stefani ◽

...

Keyword(s):

Southern Italy ◽

Molecular Study ◽

Molecular Data ◽

Morphological Data ◽

Ecological Knowledge ◽

Mitochondrial Coi ◽

Copepodid Stage ◽

Morphological And Molecular Data ◽

Morphological And Molecular Characterization

We describe three new parastenocaridid: Cottarellicaris sanctiangeli Bruno & Cottarelli sp. nov., Stammericaris vincentimariae Bruno & Cottarelli sp. nov. and Proserpinicars specincola Bruno & Cottarelli sp. nov., collected in the pools of five different caves located in Calabria (Southern Italy). We conducted a phylogenetic analysis based on the mitochondrial COI and ribosomal 18S sequences of C. sanctiangeli sp. nov. and S. vincentimariae sp. nov., and of four more species of Stammericaris and one Proserpinicaris available from literature. Based on the molecular study, the specimens of C. sanctiangeli sp. nov. are clearly separated from the species belonging to the closely-related genus Stammericaris. The morphological and molecular data indicate that the genera belonging to the two subfamilies Parastenocaridinae and Fontinalicaridinae form two monophyletic and distinct clades, thus supporting their status. We also provide insights on the genus Proserpinicaris based on morphological data only; in particular, the most important synapomorphic character of the genus, i.e., the taxonomic value of the hyaline structure inserted on the anterior surface of the male leg 4 basis, is discussed based on the development of the P4 endopod, as observed in the last copepodid stage of some species of this genus. Finally, we widen the biogeographic and ecological knowledge of the three genera.

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Morphological and molecular characterization of Termitomyces (Lyophyllaceae, Agaricales) in Thailand

Biodiversitas Journal of Biological Diversity ◽

10.13057/biodiv/d210620 ◽

2020 ◽

Vol 21 (6) ◽

Cited By ~ 1

Author(s):

NICHAREE JANNUAL ◽

MINGKWAN NIPITWATTANAPHON ◽

SASITORN HASIN ◽

Tharnrat Kaewgrajang

Keyword(s):

Molecular Characterization ◽

Morphological Characteristics ◽

Molecular Data ◽

Morphological Data ◽

Molecular Characteristics ◽

Its2 Region ◽

Diagnostic Key ◽

Morphological And Molecular Characterization ◽

Home Consumption

Abstract. Jannual N, Nipitwattanaphon M, Hasin S, Kaewgrajang T. 2020. Morphological and molecular characterization of Termitomyces (Lyophyllaceae, Agaricales) in Thailand. Biodiversitas 21: 2481-2491. Termitomyces is considered to be a highly prized delicacy collected both for home consumption and for sale in local markets. Although the taxonomic information about this genus is well known in Africa, the identification of Termitomyces species in Thailand is unclear. Therefore, this study presented an assessment of phylogenetic relationships in the genus Termitomyces by means of sequencing of the ITS1-5.8S-ITS2 region and the species characterization by combination of morphological data and molecular data. Total of 61 Termitomyces specimens was collected from a variety of geographical localities of Thailand. They were classified into six species, including T. clypeatus, T. cylindricus, T. fuliginosus, T. heimii, T. microcarpus, and T. striatus based on morphological characteristics combining with molecular characteristics. This is the first diagnostic key of Thai Termitomyces.

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Morphological and Molecular Characterization of Brasilonema roberti-lamii (Cyanophyceae, Nostocales, Scytonemataceae), from Central Mexico

Phytotaxa ◽

10.11646/phytotaxa.164.4.4 ◽

2014 ◽

Vol 164 (4) ◽

pp. 255 ◽

Cited By ~ 8

Author(s):

BEATRIZ RODARTE ◽

ITZEL BECERRA-ABSALÓN ◽

GUSTAVO A. MONTEJANO ◽

KARINA OSORIO-SANTOS ◽

LUISA ALBA-LOIS ◽

...

Keyword(s):

Molecular Characterization ◽

Growth Form ◽

Good Alternative ◽

Molecular Data ◽

Central Mexico ◽

Rrna Gene ◽

Morphological Criteria ◽

Ecological Criteria ◽

Morphological And Molecular Characterization

This paper is a contribution to the morphological and molecular characterization of the cyanobacterium Brasilonema roberti-lamii from populations found in Central Mexico. The general growth form and the morphological, morphometric and ecological characteristics of the populations studied clearly correspond to those described for Brasilonema roberti-lamii (basionym: Tolypothrix roberti-lamii) from the French Antilles. Based on molecular data from DNA sequencing of the16S rRNA gene and the IGS of the cpcB-cpcA phycocyanin operon (cpcBA-IGS), we propose that the populations that we studied are closely related to those of other Brasilonema species, including B. octagenarum UFV-OR1, UFV-E1 and HA4187-MV1-p1F, Brasilonema sennae CENA 114, B. tolantongensis, B. terrestre CENA 116, B. angustatum HA4187-MV1-B2+p1F and HA4187-MV1-B2+p1H and B. bromeliae SPC951. Our findings support the transference of Tolypothrix roberti-lamii, which was made based exclusively on morphological criteria, to Brasilonema. The use of molecular analyses in addition to traditional morphological and ecological criteria, known as polyphasic approach, is a good alternative to describe taxa of cyanobacteria, mainly at the genus and species levels.

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