Morphological and Molecular Characterization of Brasilonema roberti-lamii (Cyanophyceae, Nostocales, Scytonemataceae), from Central Mexico

This paper is a contribution to the morphological and molecular characterization of the cyanobacterium Brasilonema roberti-lamii from populations found in Central Mexico. The general growth form and the morphological, morphometric and ecological characteristics of the populations studied clearly correspond to those described for Brasilonema roberti-lamii (basionym: Tolypothrix roberti-lamii) from the French Antilles. Based on molecular data from DNA sequencing of the16S rRNA gene and the IGS of the cpcB-cpcA phycocyanin operon (cpcBA-IGS), we propose that the populations that we studied are closely related to those of other Brasilonema species, including B. octagenarum UFV-OR1, UFV-E1 and HA4187-MV1-p1F, Brasilonema sennae CENA 114, B. tolantongensis, B. terrestre CENA 116, B. angustatum HA4187-MV1-B2+p1F and HA4187-MV1-B2+p1H and B. bromeliae SPC951. Our findings support the transference of Tolypothrix roberti-lamii, which was made based exclusively on morphological criteria, to Brasilonema. The use of molecular analyses in addition to traditional morphological and ecological criteria, known as polyphasic approach, is a good alternative to describe taxa of cyanobacteria, mainly at the genus and species levels.

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Morphological and Molecular Characterization of a New Nearctic Species ofCalligraphaChevrolat, 1836 (Coleoptera: Chrysomelidae, Chrysomelinae) from Central Mexico

Proceedings of the Entomological Society of Washington ◽

10.4289/0013-8797.115.4.369 ◽

2013 ◽

Vol 115 (4) ◽

pp. 369-391 ◽

Cited By ~ 4

Author(s):

Tinguaro Montelongo ◽

Jesús Gómez-Zurita

Keyword(s):

Molecular Characterization ◽

Central Mexico ◽

Morphological And Molecular Characterization

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Morphological and molecular characterization of Termitomyces (Lyophyllaceae, Agaricales) in Thailand

Biodiversitas Journal of Biological Diversity ◽

10.13057/biodiv/d210620 ◽

2020 ◽

Vol 21 (6) ◽

Cited By ~ 1

Author(s):

NICHAREE JANNUAL ◽

MINGKWAN NIPITWATTANAPHON ◽

SASITORN HASIN ◽

Tharnrat Kaewgrajang

Keyword(s):

Molecular Characterization ◽

Morphological Characteristics ◽

Molecular Data ◽

Morphological Data ◽

Molecular Characteristics ◽

Its2 Region ◽

Diagnostic Key ◽

Morphological And Molecular Characterization ◽

Home Consumption

Abstract. Jannual N, Nipitwattanaphon M, Hasin S, Kaewgrajang T. 2020. Morphological and molecular characterization of Termitomyces (Lyophyllaceae, Agaricales) in Thailand. Biodiversitas 21: 2481-2491. Termitomyces is considered to be a highly prized delicacy collected both for home consumption and for sale in local markets. Although the taxonomic information about this genus is well known in Africa, the identification of Termitomyces species in Thailand is unclear. Therefore, this study presented an assessment of phylogenetic relationships in the genus Termitomyces by means of sequencing of the ITS1-5.8S-ITS2 region and the species characterization by combination of morphological data and molecular data. Total of 61 Termitomyces specimens was collected from a variety of geographical localities of Thailand. They were classified into six species, including T. clypeatus, T. cylindricus, T. fuliginosus, T. heimii, T. microcarpus, and T. striatus based on morphological characteristics combining with molecular characteristics. This is the first diagnostic key of Thai Termitomyces.

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Morphological and Molecular Characterization of Kochinema farodai Baqri and Bohra, 2001 (Dorylaimida: Nordiidae) from California, with the First Molecular Study and an Updated Taxonomy of the Genus

Animals ◽

10.3390/ani10122300 ◽

2020 ◽

Vol 10 (12) ◽

pp. 2300

Author(s):

Sergio Álvarez-Ortega

Keyword(s):

Molecular Characterization ◽

Molecular Study ◽

Recent Common Ancestor ◽

Integrative Approach ◽

Distribution Data ◽

Rrna Gene ◽

28S Rrna ◽

The Family ◽

Morphological And Molecular Characterization

This paper deals with the morphological and molecular characterization of Kochinema farodai Baqri and Bohra, 2001, with an integrative approach. The finding of K. faroidai in California is a remarkable biogeographical novelty, as it is the first American record of the species. Molecular data herein obtained represent the first molecular study of the genus Kochinema. Furthermore, scanning electron microscopy (SEM) observations of a member of Kochinema are provided for the first time. Additionally, this contribution provides new insights into the phylogeny and taxonomy of the nematode genus Kochinema. A brief historical outline of the matter is presented. Then, the morphological pattern of the genus is revised and illustrated, the anterior position of amphids, whose opening is located on lateral lip, being its most relevant diagnostic feature. The phylogenetic analysis inferred from D2–D3 expansion segments of 28S rRNA gene shows that Kochinema clustered together with other dorylaimid species characterized by the absence of pars refringens vaginae and that it does not share a recent common ancestor with other members of the family Nordiidae. A likely polyphyly of the family Nordiidae is confirmed. Finally, an updated taxonomy of the genus is proposed, including a revised diagnosis, a list of species, a key to species identification, and a compendium of their main morphometrics and distribution data.

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