scholarly journals Information System for Selection of Conditions and Equipment for Mammalian Cell Cultivation

Data ◽  
2021 ◽  
Vol 6 (3) ◽  
pp. 23
Author(s):  
Natalia Menshutina ◽  
Elena Guseva ◽  
Diana Batyrgazieva ◽  
Igor Mitrofanov
Keyword(s):  
Cell Culture ◽  
Information System ◽  
Mammalian Cell ◽  
Mammalian Cells ◽  
Scientific Research ◽  
Modern Concept ◽  
Successful Implementation ◽  
Cell Cultivation ◽  
Mammalian Cell Cultivation ◽  
Culture Technology

Over the past few decades, animal cell culture technology has advanced significantly. It is now considered a reliable, functional, and relatively well-developed technology. At present, biotherapeutic drugs are synthesized using cell culture techniques by large manufacturing enterprises that produce products for commercial use and clinical research. The reliable implementation of mammalian cell culture technology requires the optimization of a number of variables, including the culture environment and bioreactor conditions, suitable cell lines, operating costs, efficient process management and, most importantly, quality. Successful implementation also requires an appropriate process development strategy, industrial scale, and characteristics, as well as the certification of sustainable procedures that meet the requirements of current regulations. All of this has led to a trend of increasing research in the field of biotechnology and, as a result, to a great accumulation of scientific information which, however, remains fragmentary and non-systematic. The development of information and network technologies allow us to solve this problem. Information system creation allows for implementation of the modern concept of integrating various structured and unstructured data, as well as the collection of information from internal and external sources. We propose and develop an information system which contains the conditions and various parameters of cultivation processes. The associated ranking system is the result of the set of recommendations—both from technological and hardware solutions—which allow for choosing the optimal conditions for the cultivation of mammalian cells at the stage of scientific research, thereby significantly reducing the time and cost of work. The proposed information system allows for the accumulation of experience regarding existing technologies for the cultivation of mammalian cells, along with application to the development of new technologies. The main goal of the present work is to discuss information systems, the organizational support of scientific research in the field of mammalian cell cultivation, and to provide a detailed description of the developed system and its main modules, including the conceptual and logical scheme of the database.

Disposable 600-mL orbitally shaken bioreactor for mammalian cell cultivation in suspension

2013 ◽  
Vol 76 ◽  
pp. 6-12 ◽  
Author(s):  
Dominique T. Monteil ◽  
Giulia Tontodonati ◽  
Saroj Ghimire ◽  
Lucia Baldi ◽  
David L. Hacker ◽  
...  
Keyword(s):  
Mammalian Cell ◽  
Cell Cultivation ◽  
Mammalian Cell Cultivation

Influenza Vaccines – Challenges in Mammalian Cell Culture Technology

2007 ◽  
pp. 503-508 ◽  
Author(s):  
Yvonne Genzel ◽  
Josef Schulze-Horsel ◽  
Lars Möhler ◽  
Yury Sidorenko ◽  
Udo Reichl
Keyword(s):  
Cell Culture ◽  
Mammalian Cell ◽  
Mammalian Cell Culture ◽  
Influenza Vaccines ◽  
Culture Technology

In-situ near infrared spectroscopy to monitor key analytes in mammalian cell cultivation

2003 ◽  
Vol 84 (1) ◽  
pp. 13-19 ◽  
Author(s):  
S. Alison Arnold ◽  
John Crowley ◽  
Nigel Woods ◽  
Linda M. Harvey ◽  
Brian McNeil
Keyword(s):  
Infrared Spectroscopy ◽  
Mammalian Cell ◽  
Near Infrared Spectroscopy ◽  
Near Infrared ◽  
Cell Cultivation ◽  
Mammalian Cell Cultivation

Automated imunoanalysis systems for monitoring mammalian cell cultivation processes

1994 ◽  
Vol 15 (1-3) ◽  
pp. 259-269 ◽  
Author(s):  
Birgitt Schulze ◽  
Cornelia Middendorf ◽  
Martin Reinecke ◽  
Thomas Scheper ◽  
Wolfgang No� ◽  
...  
Keyword(s):  
Mammalian Cell ◽  
Cell Cultivation ◽  
Mammalian Cell Cultivation

scholarly journals S-Trap eliminates cell culture media polymeric surfactants for effective proteomic analysis of mammalian cell bioreactor supernatants

2020 ◽  
Author(s):  
Lucia F. Zacchi ◽  
Dinora Roche Recinos ◽  
Ellen Otte ◽  
Campbell Aitken ◽  
Tony Hunt ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Cell Culture ◽  
Sample Preparation ◽  
Mammalian Cell ◽  
Proteomic Analysis ◽  
Mammalian Cells ◽  
Culture Media ◽  
Quality Data ◽  
Polymeric Surfactants ◽  
Cell Culture Media

AbstractProteomic analysis of bioreactor supernatants can inform on cellular metabolic status, viability, and productivity, as well as product quality, which can in turn help optimize bioreactor operation. Incubating mammalian cells in bioreactors requires the addition of polymeric surfactants such as Pluronic F68, which reduce the sheer stress caused by agitation. However, these surfactants are incompatible with mass spectrometry proteomics and must be eliminated during sample preparation. Here, we compared four different sample preparation methods to eliminate polymeric surfactants from filtered bioreactor supernatant samples: organic solvent precipitation; filter-assisted sample preparation (FASP); S-Trap; and single-pot, solid-phase, sample preparation (SP3). We found that SP3 and S-Trap substantially reduced or eliminated the polymer(s), but S-Trap provided the most robust clean-up and highest quality data. Additionally, we observed that SP3 sample preparation of our samples and in other published datasets was associated with partial alkylation of cysteines, which could impact the confidence and robustness of protein identification and quantification. Finally, we observed that several commercial mammalian cell culture media and media supplements also contained polymers with similar mass spectrometry profiles, and we suggest that proteomic analyses in these media will also benefit from the use of S-Trap sample preparation.

scholarly journals Synthetic auxotrophy remains stable after continuous evolution and in co-culture with mammalian cells

2020 ◽  
Author(s):  
Aditya M. Kunjapur ◽  
Michael G. Napolitano ◽  
Eriona Hysolli ◽  
Karen Noguera ◽  
Evan M. Appleton ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Cell Culture ◽  
Mammalian Cell ◽  
Mammalian Cells ◽  
Mammalian Cell Culture ◽  
Normal Growth ◽  
Evolutionary Stability ◽  
Continuous Evolution ◽  
Molecule Transport ◽  
Small Molecule Transport

AbstractUnderstanding the evolutionary stability and possible context-dependence of biological containment techniques is critical as engineered microbes are increasingly under consideration for applications beyond biomanufacturing. While batch cultures of synthetic auxotrophic Escherichia coli previously exhibited undetectable escape throughout 14 days of monitoring, the long-term effectiveness of synthetic auxotrophy is unknown. Here, we report automated continuous evolution of a synthetic auxotroph using custom chemostats that supply a decreasing concentration of essential biphenylalanine (BipA). After 100 days of evolution in three separate trials, populations exhibit no observable escape and are capable of normal growth rates at 10-fold lower BipA concentration than the ancestral synthetic auxotroph. Allelic reconstruction of three proteins implicated in small molecule transport reveals their contribution to increased fitness at low BipA concentrations. Mutations do not appear in orthogonal translation machinery nor in synthetic auxotrophic markers. Based on its evolutionary stability, we introduce the progenitor synthetic auxotroph directly to mammalian cell culture. We observe containment of bacteria without detrimental effects on HEK293T cells. Overall, our findings reveal that synthetic auxotrophy is effective on timescales and in contexts that enable diverse applications.One Sentence SummaryTo ascertain whether life inevitably finds a way, we continuously evolve an Escherichia coli strain that was not able to escape from engineered biocontainment before, and we find that it does not escape even after 100 days of evolution, nor does it escape when added to mammalian cell culture.

scholarly journals Mammalian Cell Culture Clarification: A Case Study Using Chimeric Anti-CEA Monoclonal Antibodies

2011 ◽  
Vol 12 (4) ◽  
Author(s):  
Mohamed Ali Abol Hassan ◽  
Abdul Wahab Mohammad ◽  
And Badarulhisam Abdul Rahman
Keyword(s):  
Cell Culture ◽  
Monoclonal Antibodies ◽  
Host Cell ◽  
Cell Lines ◽  
Mammalian Cell ◽  
Mammalian Cells ◽  
Culture Media ◽  
Mammalian Cell Culture ◽  
Host Cell Proteins ◽  
Depth Filtration

The extracellular expression of monoclonal antibodies (mAbs) in mammalian cell culture provides both opportunities and restrictions for the design of robust harvest and clarification operations. With advances in cell culture media and cell lines, it is now possible to achieve high titers of over 5 g/l for mAbs. However, Mammalian cells are sensitive to breakage due to shear stress that can result in release of proteases and other host cell proteins (HCPs) which eventually affects product stability and purity. There is larger number of mAbs undergoing clinical development and it has placed significant importance on platform technologies of process development. Generally, Centrifugation and microfiltration are the primary harvest techniques used in the industry and depth filtration is also used as a step operation on clarification. This study compares the unit operations; centrifugation, microfiltration and depth filtration for maximum recovery of monoclonal antibodies. The results have shown that the depth filtration as more suitable operation for mammalian cell culture clarification since it gives 96% recovery of mAbs in comparison to centrifugation and microfiltration. ABSTRAK: Pengungkapan luar sel dari antibodi monoklon (monoclonal antibodies ((mAbs) dalam kultur sel mamalia memberi ruang dan batasan terhadap reka bentuk penuaian yang cekap dan penerangan operasi. Dengan kemajuan dalam media sel kultur dan cell lines (produk yang berupa sel kekal yang digunakan untuk tujuan kajian biologi), kini adalah berkemungkinan untuk memperolehi titer tinggi melebihi 5g/l untuk mAbs [2]. Walaupun begitu, sel mamalia sensitif terhadap retakan disebabkan tegasan ricih yang menyebabkan pengeluaran protease dan hos sel protein yang lain, (host cell proteins (HCPs)) akhirnya mempengaruhi kestabilan dan keaslian produk. Terdapat mAbs dalam jumlah besar yang masih menjalani pembangunan klinikal dan sesungguhnya ini penting sebagai satu landasan teknologi dalam proses pembangunan. Umumnya pengemparan dan mikropenurasan merupakan teknik asas tuaian dalam industri dan penurasan dalam juga digunakan sebagai satu pengendalian langkah dalam penjelasannya. Kajian ini membandingkan operasi unit: pengemparan, mikropenurasan dan penurasan dalam untuk perolehan antibodi monoklon yang maksima. Keputusan menunjukkan penurasan dalam adalah operasi yang lebih sesuai untuk penjelasan kultur sel mamalia kerana ia memberikan perolehan 96 % mAbs berbandingkan dengan cara pengemparan dan mikropenurasan.

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