Potential Role of Hydroxyapatite Nanocrystalline for Early Diagnostics of Ovarian Cancer

Calcification is one of the clinical and morphological manifestations of ovarian tumors and it begins at the initial stages of carcinogenesis. Thus, this process can be used for the early diagnostics of some malignant ovarian tumors. We compared the results of ultrasound and histology and found that calcifications of a size less than 200 μm are not detected by ultrasound. These calcified structures are round fragile particles of different sizes. In the EDX (Energy-dispersive X-ray spectroscopy) spectra, the main lines were from Ca and P, and the ratio of these elements corresponds to hydroxyapatite. Thus, we established that hydroxyapatite is the main mineral component of ovarian psammoma bodies and could be used for early diagnostics of ovarian malignant neoplasia.

Download Full-text

Electron Energy-Loss and Energy Dispersive X-Ray Spectros-Copy Studies of Diffusion Bonded Cu-Al2O3 Interfaces

Microscopy and Microanalysis ◽

10.1017/s1431927600011855 ◽

1997 ◽

Vol 3 (S2) ◽

pp. 993-994

Author(s):

R. Hashimoto ◽

E. S. K. Menon ◽

M. Saunders ◽

A. G. Fox

Keyword(s):

Grain Boundary ◽

Energy Loss ◽

Electron Energy ◽

Potential Role ◽

Energy Dispersive ◽

Commercial Alumina ◽

X Ray ◽

Metal Ceramic ◽

Electron Energy Loss

The metal-ceramic interface between copper and alumina has been studied by TEM, Electron Energy-Loss Spectroscopy (EELS), and Energy Dispersive X-ray spectroscopy (EDX). of partic-ular interest is the potential role of silicon which is the major impurity in commercial alumina. This investigation focuses on the presence and distribution of silicon within the bulk alumina and at the diffusion bonded CU-AI2O3 interface using a Topcon 002B TEM equipped with an EDAX EDX detector and a Gatan Imaging Filter. The interface was created under vacuum by diffusion bonding of 100/xm copper foils (99.999% purity) pressed between polished alumina substrates (∼99.5% purity) for several hours at ∼90% of the melting temperature of the metal.An initial investigation of the bulk AI2O3 indicated that the majority of the Si occurred at the triple points. A typical example is shown in Fig. la. EDX spectra acquired with a 6nm probe from the center of the junction (Fig. lbi) and at the grain boundary indicate that the Si wets the grain boundary to a limited extent.

Download Full-text

Correction: Potential Role of Estrogen Receptor Beta as a Tumor Suppressor of Epithelial Ovarian Cancer

PLoS ONE ◽

10.1371/annotation/480acc26-456b-4e06-8cb6-2834bd6f5553 ◽

2013 ◽

Vol 8 (5) ◽

Author(s):

Carine Bossard ◽

Muriel Busson ◽

David Vindrieux ◽

FranÃ§oise Gaudin ◽

VÃ©ronique Machelon ◽

...

Keyword(s):

Ovarian Cancer ◽

Estrogen Receptor ◽

Tumor Suppressor ◽

Epithelial Ovarian Cancer ◽

Potential Role ◽

Estrogen Receptor Beta ◽

Receptor Beta ◽

Correction Potential

Download Full-text

Role of medical-genetic consultations in early diagnostics of ovarian tumors in girls

Journal of Pediatric and Adolescent Gynecology ◽

10.1016/s1083-3188(01)00095-x ◽

2001 ◽

Vol 14 (3) ◽

pp. 146

Author(s):

A. Peresunko

Keyword(s):

Ovarian Tumors ◽

Medical Genetic ◽

Early Diagnostics

Download Full-text

Cryo-scanning electron microscopy (CSEM) in the advancement of functional plant biology: energy dispersive X-ray microanalysis (CEDX) applications

Functional Plant Biology ◽

10.1071/fp10095 ◽

2010 ◽

Vol 37 (11) ◽

pp. 1011 ◽

Cited By ~ 39

Author(s):

Margaret E. McCully ◽

Martin J. Canny ◽

Cheng X. Huang ◽

Celia Miller ◽

Frank Brink

Keyword(s):

Single Cells ◽

Energy Dispersive ◽

Intercellular Spaces ◽

Root Pressure ◽

Solute Content ◽

X Ray ◽

Plant Parts ◽

Specific Analysis ◽

Vesicular Arbuscular

The capacity to make measurements of elemental concentrations at the level of single cells by energy dispersive X-ray microanalysis of cryo-fixed, inherently-hydrated plant parts (CEDX) is changing or extending our understanding of many plant functions. We include in this review a wide-ranging catalogue of studies that have used CEDX which provides access to the literature on elements measured, plants and tissues studied, techniques used, level of quantitation and the significant findings. These findings include new perspectives on the following areas: salt tolerance; xylem maturation and solute content, root pressure and embolism refilling; the contents of intercellular spaces; sequestration of toxic elements; biomineralisation with silicon; movement of tracer homologues of native cations; indirect localisation of molecules with a distinctive element component; transfer of nutrients from vesicular-arbuscular (VA) mycorrhizas; the role of mucilages in protection and in generating mechanical force. In an Appendix we discuss the procedures involved in CEDX: cryo-fixation, specimen planing, etching, elemental quantitation and mapping. Limitations on sample numbers, elements measurable, spatial resolution, sensitivity and threshold concentrations quantifiable are outlined. A brief discussion of the potential of emerging technologies for cell-specific analysis of cryo-fixed, hydrated specimens is included. In the Accessory Publication we list our standard protocol for CEDX.

Download Full-text

Potential Role of Estrogen Receptor Beta as a Tumor Suppressor of Epithelial Ovarian Cancer

PLoS ONE ◽

10.1371/journal.pone.0044787 ◽

2012 ◽

Vol 7 (9) ◽

pp. e44787 ◽

Cited By ~ 60

Author(s):

Carine Bossard ◽

Muriel Busson ◽

David Vindrieux ◽

Françoise Gaudin ◽

Véronique Machelon ◽

...

Keyword(s):

Ovarian Cancer ◽

Estrogen Receptor ◽

Tumor Suppressor ◽

Epithelial Ovarian Cancer ◽

Potential Role ◽

Estrogen Receptor Beta ◽

Receptor Beta

Download Full-text

Potential Role of HE4 in Multimodal Screening for Epithelial Ovarian Cancer

JNCI Journal of the National Cancer Institute ◽

10.1093/jnci/djr359 ◽

2011 ◽

Vol 103 (21) ◽

pp. 1630-1634 ◽

Cited By ~ 36

Author(s):

N. Urban ◽

J. D. Thorpe ◽

L. A. Bergan ◽

R. M. Forrest ◽

A. V. Kampani ◽

...

Keyword(s):

Ovarian Cancer ◽

Epithelial Ovarian Cancer ◽

Potential Role

Download Full-text

Potential Role of Coregistered Photoacoustic and Ultrasound Imaging in Ovarian Cancer Detection and Characterization

Translational Oncology ◽

10.1593/tlo.10187 ◽

2011 ◽

Vol 4 (1) ◽

pp. 29-37 ◽

Cited By ~ 47

Author(s):

Andres Aguirre ◽

Yasaman Ardeshirpour ◽

Mary M. Sanders ◽

Molly Brewer ◽

Quing Zhu

Keyword(s):

Ovarian Cancer ◽

Ultrasound Imaging ◽

Cancer Detection ◽

Potential Role

Download Full-text

The potential role of miRNAs and exosomes in chemotherapy in ovarian cancer

Endocrine Related Cancer ◽

10.1530/erc-18-0019 ◽

2018 ◽

Vol 25 (12) ◽

pp. R663-R685 ◽

Cited By ~ 15

Author(s):

Mona Alharbi ◽

Felipe Zuñiga ◽

Omar Elfeky ◽

Dominic Guanzon ◽

Andrew Lai ◽

...

Keyword(s):

Ovarian Cancer ◽

Disease Progression ◽

Potential Role ◽

Chemotherapy Resistance ◽

Predictive Biomarker ◽

Response To Therapy ◽

Target Cells ◽

Repair Mechanisms ◽

Drug Efflux Pumps

Chemoresistance is one of the major obstacles in the treatment of cancer patients. It poses a fundamental challenge to the effectiveness of chemotherapy and is often linked to relapse in patients. Chemoresistant cells can be identified in different types of cancers; however, ovarian cancer has one of the highest rates of chemoresistance-related relapse (50% of patients within 5 years). Resistance in cells can either develop through prolonged cycles of treatment or through intrinsic pathways. Mechanistically, the problem of drug resistance is complex mainly because numerous factors are involved, such as overexpression of drug efflux pumps, drug inactivation, DNA repair mechanisms and alterations to and/or mutations in the drug target. Additionally, there is strong evidence that circulating miRNAs participate in the development of chemoresistance. Recently, miRNAs have been identified in exosomes, where they are encapsulated and hence protected from degradation. These miRNAs within exosomes (exo-miRNAs) can regulate the gene expression of target cells both locally and systemically. Exo-miRNAs play an important role in disease progression and can potentially facilitate chemoresistance in cancer cells. In addition, and from a diagnostic perspective, exo-miRNAs profiles may contribute to the development of predictive models to identify responder and non-responder chemotherapy. Such model may also be used for monitoring treatment response and disease progression. Exo-miRNAs may ultimately serve as both a predictive biomarker for cancer response to therapy and as a prognostic marker for the development of chemotherapy resistance. Therefore, this review examines the potential role of exo-miRNAs in chemotherapy in ovarian cancer.

Download Full-text