scholarly journals Salivary Exosome Proteomics and Bioinformatics Analysis in 7,12-Dimethylbenz[a]anthracene-induced Oral Cancer with Radiation Therapy—A Syrian Golden Hamster Model

Diagnostics ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 65
Author(s):  
Wen-Chen Wang ◽  
Yuk-Kwan Chen ◽  
Ming-Yii Huang ◽  
Tzong-Ming Shieh ◽  
Wan-Chen Lan ◽  
...  
Keyword(s):  
Radiation Therapy ◽  
Golden Hamster ◽  
Tumor Tissue ◽  
Treated Group ◽  
Syrian Golden Hamster ◽  
Main Role ◽  
Bioinformatics Analyses ◽  
Hamster Model ◽  
P53 Expression

Exosomes carry cellular proteins and contain molecules that can be potential biomarkers of diseases. This study used a Syrian golden hamster model of 7,12-dimethylbenz[a]anthracene (DMBA)-induced oral squamous cell carcinoma with radiation therapy to exclude the confounding factors that may affect outcomes in clinical studies, and re-examine the role of exosomes during tumorigenesis. We used data-dependent acquisition-based quantitative proteomics and bioinformatics analyses and found unique proteins present (desmocollin-2) or absent (Glucagon-cAMP-PKA-CREB pathway-related proteins) in the salivary exosomes of the pre-radiation DMBA-treated group (PreD). Comparing our data to other studies, salivary exosomes in the PreD group were found carrying proteins that the tumor mass does not express and lacking the proteins needed during tumorigenesis. Immunohistochemistry staining showed p53 expression but a negative apoptotic signal in the PreD tumor tissue. We thus suggest that inhibition of desmocollin-2 expression in tumor tissue may impede the activation of cell apoptosis. However, both the origin of the salivary exosomes and main role of the salivary exosome proteins should be clarified in future studies.

scholarly journals Molecular detection of infection homogeneity and impact of miltefosine treatment in a Syrian golden hamster model of Leishmania donovani and L. infantum visceral leishmaniasis

2016 ◽  
Vol 115 (10) ◽  
pp. 4061-4070 ◽  
Author(s):  
Eline Eberhardt ◽  
Annelies Mondelaers ◽  
Sarah Hendrickx ◽  
Magali Van den Kerkhof ◽  
Louis Maes ◽  
...  
Keyword(s):  
Visceral Leishmaniasis ◽  
Golden Hamster ◽  
Leishmania Donovani ◽  
Molecular Detection ◽  
Syrian Golden Hamster ◽  
Hamster Model

Implantation and Monitoring of a Novel Telemetry Unit in the Syrian Golden Hamster Model

2012 ◽  
Vol 25 (3) ◽  
pp. 186-196 ◽  
Author(s):  
Jennifer Garver ◽  
Oscar A. Bermeo-Blanco ◽  
Neil Gibson ◽  
Heather Bogie ◽  
Jessica Grenwis ◽  
...  
Keyword(s):  
Golden Hamster ◽  
Syrian Golden Hamster ◽  
Hamster Model ◽  
Telemetry Unit

scholarly journals Pathogenic and transcriptomic differences of emerging SARS-CoV-2 variants in the Syrian golden hamster model

EBioMedicine ◽  
2021 ◽  
Vol 73 ◽  
pp. 103675
Author(s):  
Kyle L. O'Donnell ◽  
Amanda N. Pinski ◽  
Chad S. Clancy ◽  
Tylisha Gourdine ◽  
Kyle Shifflett ◽  
...  
Keyword(s):  
Golden Hamster ◽  
Syrian Golden Hamster ◽  
Hamster Model

Genistein, a general kinase inhibitor, as a potential antiviral for arenaviral hemorrhagic fever as described in the Pirital virus-Syrian golden hamster model

2010 ◽  
Vol 87 (3) ◽  
pp. 318-328 ◽  
Author(s):  
Eric M. Vela ◽  
Katherine A. Knostman ◽  
Jason M. Mott ◽  
Richard L. Warren ◽  
Jennifer N. Garver ◽  
...  
Keyword(s):  
Golden Hamster ◽  
Kinase Inhibitor ◽  
Hemorrhagic Fever ◽  
Syrian Golden Hamster ◽  
Hamster Model

scholarly journals An immunosuppressed Syrian golden hamster model for SARS-CoV infection

Virology ◽  
2008 ◽  
Vol 380 (2) ◽  
pp. 312-321 ◽  
Author(s):  
Scott R. Schaecher ◽  
Jennifer Stabenow ◽  
Christina Oberle ◽  
Jill Schriewer ◽  
R. Mark Buller ◽  
...  
Keyword(s):  
Golden Hamster ◽  
Syrian Golden Hamster ◽  
Hamster Model

scholarly journals A Syrian Golden Hamster Model Recapitulating Ebola Hemorrhagic Fever

2012 ◽  
Vol 207 (2) ◽  
pp. 306-318 ◽  
Author(s):  
Hideki Ebihara ◽  
Marko Zivcec ◽  
Donald Gardner ◽  
Darryl Falzarano ◽  
Rachel LaCasse ◽  
...  
Keyword(s):  
Golden Hamster ◽  
Hemorrhagic Fever ◽  
Syrian Golden Hamster ◽  
Hamster Model ◽  
Ebola Hemorrhagic Fever

Role of spectrin in membrane fusion and in shape change of human erythrocyte ghost

1978 ◽  
Vol 36 (2) ◽  
pp. 328-329
Author(s):  
Hideo Hayashi ◽  
Yoshikazu Hirai ◽  
John T. Penniston
Keyword(s):  
Conformational Changes ◽  
Human Erythrocyte ◽  
Shape Change ◽  
Membrane Surface ◽  
Slight Modification ◽  
Active Role ◽  
Main Role ◽  
Bank Blood ◽  
Human Erythrocyte Ghost

Spectrin is a membrane associated protein most of which properties have been tentatively elucidated. A main role of the protein has been assumed to give a supporting structure to inside of the membrane. As reported previously, however, the isolated spectrin molecule underwent self assemble to form such as fibrous, meshwork, dispersed or aggregated arrangements depending upon the buffer suspended and was suggested to play an active role in the membrane conformational changes. In this study, the role of spectrin and actin was examined in terms of the molecular arrangements on the erythrocyte membrane surface with correlation to the functional states of the ghosts.Human erythrocyte ghosts were prepared from either freshly drawn or stocked bank blood by the method of Dodge et al with a slight modification as described before. Anti-spectrin antibody was raised against rabbit by injection of purified spectrin and partially purified.

Mesophyll conductance: the leaf corridors for photosynthesis

2020 ◽  
Vol 48 (2) ◽  
pp. 429-439 ◽  
Author(s):  
Jorge Gago ◽  
Danilo M. Daloso ◽  
Marc Carriquí ◽  
Miquel Nadal ◽  
Melanie Morales ◽  
...  
Keyword(s):  
Molecular Mechanisms ◽  
Current Knowledge ◽  
Main Role ◽  
Mesophyll Conductance ◽  
Future Studies ◽  
Cell Structures ◽  
Leaf Tissues ◽  
Change Framework ◽  
Chloroplast Stroma

Besides stomata, the photosynthetic CO2 pathway also involves the transport of CO2 from the sub-stomatal air spaces inside to the carboxylation sites in the chloroplast stroma, where Rubisco is located. This pathway is far to be a simple and direct way, formed by series of consecutive barriers that the CO2 should cross to be finally assimilated in photosynthesis, known as the mesophyll conductance (gm). Therefore, the gm reflects the pathway through different air, water and biophysical barriers within the leaf tissues and cell structures. Currently, it is known that gm can impose the same level of limitation (or even higher depending of the conditions) to photosynthesis than the wider known stomata or biochemistry. In this mini-review, we are focused on each of the gm determinants to summarize the current knowledge on the mechanisms driving gm from anatomical to metabolic and biochemical perspectives. Special attention deserve the latest studies demonstrating the importance of the molecular mechanisms driving anatomical traits as cell wall and the chloroplast surface exposed to the mesophyll airspaces (Sc/S) that significantly constrain gm. However, even considering these recent discoveries, still is poorly understood the mechanisms about signaling pathways linking the environment a/biotic stressors with gm responses. Thus, considering the main role of gm as a major driver of the CO2 availability at the carboxylation sites, future studies into these aspects will help us to understand photosynthesis responses in a global change framework.

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