scholarly journals Ovarian Cancer Targeting Phage for In Vivo Near-Infrared Optical Imaging

Diagnostics ◽  
2019 ◽  
Vol 9 (4) ◽  
pp. 183 ◽  
Author(s):  
Mallika Asar ◽  
Jessica Newton-Northup ◽  
Susan Deutscher ◽  
Mette Soendergaard
Keyword(s):  
Ovarian Cancer ◽  
Optical Imaging ◽  
Near Infrared ◽  
Fluorescent Microscopy ◽  
Reticuloendothelial System ◽  
Detection Methods ◽  
Biodistribution Studies ◽  
Late Stages ◽  
Background Contrast

Ovarian cancer is often diagnosed at late stages due to current inadequate detection. Therefore, the development of new detection methods of ovarian cancer is needed. This may be achieved by phage nanoparticles that display targeting peptides for optical imaging. Here, two such phage clones are reported. Ovarian cancer binding and specificity of phage clones (pJ18, pJ24) and peptides (J18, J24) were investigated using fluorescent microscopy and modified ELISA. Further, AF680-labeled phage particles were subjected to biodistribution and optical imaging studies in SKOV-3 xenografted mice. Fluorescent microscopy and ELISA of phage and peptides showed significantly increased binding to SKOV-3 cells compared to controls. Additionally, these studies revealed that J18 exhibits specificity for ovarian cancer SKOV-3 and OVCAR-3 cell lines. Further, peptides displayed increased SKOV-3 binding compared to N35 (non-relevant peptide) with EC50 values of 22.2 ± 10.6 μM and 29.0 ± 6.9 (mean ± SE), respectively. Biodistribution studies of AF680-labeled phage particles showed tumor uptake after 4 h and excretion through the reticuloendothelial system. Importantly, SKOV-3 tumors were easily localized by optical imaging after 2 h and 4 h and displayed good tumor-to-background contrast. The fluorescent tumor signal intensity was significantly higher for pJ18 compared to wild type (WT) after 2 h.

scholarly journals Ovarian Cancer Targeting Phage Clones for In Vivo Near‐Infrared Optical Imaging

2018 ◽  
Vol 32 (S1) ◽  
Author(s):  
Mallika Asar ◽  
Jessica Newton‐Northup ◽  
Susan Deutscher ◽  
Mette Soendergaard
Keyword(s):  
Ovarian Cancer ◽  
Optical Imaging ◽  
Near Infrared ◽  
Cancer Targeting

scholarly journals In Vivo Biodistribution and Lifetime Analysis of Cy5.5-Conjugated Rituximab in Mice Bearing Lymphoid Tumor Xenograft Using Time-Domain Near-Infrared Optical Imaging

2008 ◽  
Vol 7 (6) ◽  
pp. 7290.2008.00028 ◽  
Author(s):  
Stefania Biffi ◽  
Chiara Garrovo ◽  
Paolo Macor ◽  
Claudio Tripodo ◽  
Sonia Zorzet ◽  
...  
Keyword(s):  
B Cell ◽  
Optical Imaging ◽  
Time Domain ◽  
Near Infrared ◽  
Ex Vivo ◽  
Tumor Xenograft ◽  
Tumor Uptake ◽  
Biodistribution Studies

Rituximab is a chimeric monoclonal antibody directed against human CD20 antigen, which is expressed on B-cell lymphocytes and on the majority of B-cell lymphoid malignancies. Herein we report the conjugate of rituximab with the near-infrared (NIR) fluorophore Cy5.5 (RI-Cy5.5) as a tool for in vitro, in vivo, and ex vivo NIR time-domain (TD) optical imaging. In vitro, RI-Cy5.5 retained biologic activity and led to elevated cell-associated fluorescence on tumor cells. In vivo, TD optical imaging analysis of RI-Cy5.5 injected into lymphoma-bearing mice revealed a slow tumor uptake and a specific long-lasting persistence of the probe within the tumor. Biodistribution studies after intraperitoneal and endovenous administration were undertaken to evaluate differences in the tumor uptake. RI-Cy5.5 concentration in the organs after intraperitoneal injection was not as high as after endovenous injection. Ex vivo analysis of biologic tissues and organs by both TD optical imaging and immunohistochemistry confirmed the probe distribution, as demonstrated by imaging experiment in vivo, showing that RI-Cy5.5 selectively accumulated in the tumor tissue and major excretion organs. In summary, the study indicates that NIR TD optical imaging is a powerful tool for rituximab-targeting investigation, furthering understanding of its administration outcome in lymphoma treatment.

scholarly journals Molecular imaging and deep learning analysis of uMUC1 expression in response to chemotherapy in an orthotopic model of ovarian cancer

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Hongwei Zhao ◽  
Hasaan Hayat ◽  
Xiaohong Ma ◽  
Daguang Fan ◽  
Ping Wang ◽  
...  
Keyword(s):  
Neural Networks ◽  
Ovarian Cancer ◽  
Deep Learning ◽  
Cancer Progression ◽  
In Vivo Imaging ◽  
Near Infrared ◽  
Response To Therapy ◽  
Response To Chemotherapy

Abstract Artificial Intelligence (AI) algorithms including deep learning have recently demonstrated remarkable progress in image-recognition tasks. Here, we utilized AI for monitoring the expression of underglycosylated mucin 1 (uMUC1) tumor antigen, a biomarker for ovarian cancer progression and response to therapy, using contrast-enhanced in vivo imaging. This was done using a dual-modal (magnetic resonance and near infrared optical imaging) uMUC1-specific probe (termed MN-EPPT) consisted of iron-oxide magnetic nanoparticles (MN) conjugated to a uMUC1-specific peptide (EPPT) and labeled with a near-infrared fluorescent dye, Cy5.5. In vitro studies performed in uMUC1-expressing human ovarian cancer cell line SKOV3/Luc and control uMUC1low ES-2 cells showed preferential uptake on the probe by the high expressor (n = 3, p < .05). A decrease in MN-EPPT uptake by SKOV3/Luc cells in vitro due to uMUC1 downregulation after docetaxel therapy was paralleled by in vivo imaging studies that showed a reduction in probe accumulation in the docetaxel treated group (n = 5, p < .05). The imaging data were analyzed using deep learning-enabled segmentation and quantification of the tumor region of interest (ROI) from raw input MRI sequences by applying AI algorithms including a blend of Convolutional Neural Networks (CNN) and Fully Connected Neural Networks. We believe that the algorithms used in this study have the potential to improve studying and monitoring cancer progression, amongst other diseases.

scholarly journals Micelle-based activatable probe for in vivo near-infrared optical imaging of cancer biomolecules

2014 ◽  
Vol 10 (1) ◽  
pp. 187-195 ◽  
Author(s):  
Yoichi Shimizu ◽  
Takashi Temma ◽  
Isao Hara ◽  
Akira Makino ◽  
Ryo Yamahara ◽  
...  
Keyword(s):  
Optical Imaging ◽  
Near Infrared ◽  
Activatable Probe

scholarly journals Assessment of Endothelin-A Receptor Expression in Subcutaneous and Orthotopic Thyroid Carcinoma Xenografts in Vivo Employing Optical Imaging Methods

Endocrinology ◽  
2012 ◽  
Vol 153 (6) ◽  
pp. 2907-2918 ◽  
Author(s):  
Katrin Büther ◽  
Matthijs G. Compeer ◽  
Jo G. R. De Mey ◽  
Otmar Schober ◽  
Michael Schäfers ◽  
...  
Keyword(s):  
Thyroid Carcinoma ◽  
Optical Imaging ◽  
Near Infrared ◽  
Functional Performance ◽  
Thyroid Tumor ◽  
Receptor Expression ◽  
Imaging Methods ◽  
Carcinoma Cell Lines ◽  
Isolated Artery

Endothelin (ET) receptor dysregulation has been described in a number of pathophysiological processes, including cardiovascular disorders, renal failure, and cancer. The aim of this study was to evaluate the expression of the ET-A receptor (ETAR) in murine models of thyroid carcinoma using optical imaging methods. A recently developed near-infrared fluorescent tracer was first assessed in isolated artery preparations for its functional performance in comparison with known ETAR antagonists BQ123 and PD156707. Before evaluation of the tracer in vivo, different thyroid carcinoma cell lines were characterized with respect to their ET receptor expression by RT-PCR and autoradiography. In vivo, sc and orthotopic papillary thyroid tumor xenografts were clearly visualized by fluorescence reflectance imaging and fluorescence-mediated tomography up to 48 h after injection of the tracer. Binding specificity of the probe was demonstrated by predosing with PD156707 as a competing inhibitor. In conclusion, optical imaging with a fluorescent ETAR tracer allows the noninvasive imaging of tumor-associated ETAR expression in vivo. In the future, this technique may help surgeons to evaluate lesion dimensions in intraoperative settings (e.g. thyroidectomy).

scholarly journals Role of Fluorophore Charge on the In Vivo Optical Imaging Properties of Near-Infrared Cyanine Dye/Monoclonal Antibody Conjugates

2015 ◽  
Vol 27 (2) ◽  
pp. 404-413 ◽  
Author(s):  
Kazuhide Sato ◽  
Alexander P. Gorka ◽  
Tadanobu Nagaya ◽  
Megan S. Michie ◽  
Roger R. Nani ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Optical Imaging ◽  
Near Infrared ◽  
Cyanine Dye ◽  
Antibody Conjugates ◽  
Imaging Properties ◽  
In Vivo Optical Imaging

scholarly journals Correction: Optical imaging of ovarian cancer using a matrix metalloproteinase-3-sensitive near-infrared fluorescent probe

PLoS ONE ◽  
2018 ◽  
Vol 13 (8) ◽  
pp. e0202610
Author(s):  
Kuo-Hwa Wang ◽  
Yun-Ming Wang ◽  
Li-Hsuan Chiu ◽  
Tze-Chien Chen ◽  
Yu-Hui Tsai ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Matrix Metalloproteinase ◽  
Optical Imaging ◽  
Fluorescent Probe ◽  
Near Infrared ◽  
Matrix Metalloproteinase 3

scholarly journals Multi-targeted multi-color in vivo optical imaging in a model of disseminated peritoneal ovarian cancer

2009 ◽  
Vol 14 (1) ◽  
pp. 014023 ◽  
Author(s):  
Nobuyuki Kosaka ◽  
Mikako Ogawa ◽  
Michelle R. Longmire ◽  
Peter L. Choyke ◽  
Hisataka Kobayashi
Keyword(s):  
Ovarian Cancer ◽  
Optical Imaging ◽  
In Vivo Optical Imaging

scholarly journals Bright and stable near-infrared Pluronic–silica nanoparticles as contrast agents for in vivo optical imaging

2016 ◽  
Vol 4 (33) ◽  
pp. 5560-5566 ◽  
Author(s):  
Lesan Yan ◽  
Huiquan Wang ◽  
Anqi Zhang ◽  
Calvin Zhao ◽  
Yongping Chen ◽  
...  
Keyword(s):  
Lymph Node ◽  
Sentinel Lymph Node ◽  
Contrast Agents ◽  
Optical Imaging ◽  
Silica Nanoparticles ◽  
Near Infrared ◽  
Circulation Time ◽  
In Vivo Optical Imaging

The IR780@NPs exhibited excellent characteristics for in vivo imaging with a long circulation time and high retention in tumor and sentinel lymph node.

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