scholarly journals Diversity and Distribution of Phytophthora Species in Protected Natural Areas in Sicily

Forests ◽  
2019 ◽  
Vol 10 (3) ◽  
pp. 259 ◽  
Author(s):  
Thomas Jung ◽  
Federico La Spada ◽  
Antonella Pane ◽  
Francesco Aloi ◽  
Maria Evoli ◽  
...  
Keyword(s):  
Host Plants ◽  
Rhizosphere Soil ◽  
Stream Water ◽  
Its Region ◽  
Morphological Characters ◽  
Phytophthora Species ◽  
Riparian Ecosystems ◽  
Forest Stands ◽  
Natural Areas ◽  
Protected Natural Areas

: The aim of this study was to investigate the occurrence, diversity, and distribution of Phytophthora species in Protected Natural Areas (PNAs), including forest stands, rivers, and riparian ecosystems, in Sicily (Italy), and assessing correlations with natural vegetation and host plants. Fifteen forest stands and 14 rivers in 10 Sicilian PNAs were studied. Phytophthora isolations from soil and stream water were performed using leaf baitings. Isolates were identified using both morphological characters and sequence analysis of the internal transcribed spacer (ITS) region. A rich community of 20 Phytophthora species from eight phylogenetic clades, including three new Phytophthora taxa, was recovered (17 species in rhizosphere soil from forest stands and 12 species in rivers). New knowledge about the distribution, host associations, and ecology of several Phytophthora species was provided.

scholarly journals An Overview of Phytophthora Species Inhabiting Declining Quercus suber Stands in Sardinia (Italy)

Forests ◽  
2020 ◽  
Vol 11 (9) ◽  
pp. 971
Author(s):  
Salvatore Seddaiu ◽  
Andrea Brandano ◽  
Pino Angelo Ruiu ◽  
Clizia Sechi ◽  
Bruno Scanu
Keyword(s):  
Rhizosphere Soil ◽  
Phytophthora Cinnamomi ◽  
Stream Water ◽  
Morphological Characters ◽  
Quercus Suber ◽  
Phytophthora Species ◽  
Cork Oak ◽  
Oak Forests ◽  
Primary Role ◽  
Ecological Value

Cork oak forests are of immense importance in terms of economic, cultural, and ecological value in the Mediterranean regions. Since the beginning of the 20th century, these forests ecosystems have been threatened by several factors, including human intervention, climate change, wildfires, pathogens, and pests. Several studies have demonstrated the primary role of the oomycete Phytophthora cinnamomi Ronds in the widespread decline of cork oaks in Portugal, Spain, southern France, and Italy, although other congeneric species have also been occasionally associated. Between 2015 and 2019, independent surveys were undertaken to determine the diversity of Phytophthora species in declining cork oak stands in Sardinia (Italy). Rhizosphere soil samples were collected from 39 declining cork oak stands and baited in the laboratory with oak leaflets. In addition, the occurrence of Phytophthora was assayed using an in-situ baiting technique in rivers and streams located throughout ten of the surveyed oak stands. Isolates were identified by means of both morphological characters and sequence analysis of internal transcribed spacer (ITS) regions of ribosomal DNA. In total, 14 different Phytophthora species were detected. Phytophthora cinnamomi was the most frequently isolated species from rhizosphere soil, followed by P. quercina, P. pseudocryptogea, and P. tyrrhenica. In contrast, P. gonapodyides turned out to be the most dominant species in stream water, followed by P. bilorbang, P. pseudocryptogea, P. lacustris, and P. plurivora. Pathogenicity of the most common Phytophthora species detected was tested using both soil infestation and log inoculation methods. This study showed the high diversity of Phytophthora species inhabiting soil and watercourses, including several previously unrecorded species potentially involved in the decline of cork oak forests.

Spider mites (Acari: Tetranychidae) in protected natural areas of Serbia

2018 ◽  
Vol 23 (10) ◽  
pp. 2033
Author(s):  
Ivana Marić ◽  
Irena Međo ◽  
Slobodan Jovanović ◽  
Radmila Petanović ◽  
Dejan Marčić
Keyword(s):  
Plant Species ◽  
Host Plants ◽  
Spider Mite ◽  
Spider Mites ◽  
Mite Species ◽  
Southeast Europe ◽  
Natural Areas ◽  
Relict Species ◽  
Protected Natural Areas ◽  
New Hosts

Despite economic importance of Tetranychidae, knowledge regarding diversity of spider mites in the Balkan Peninsula and Southeast Europe is incomplete, especially in protected natural areas. This study presents diversity of spider mites (Acari: Tetranychidae) collected over five growing seasons at 296 locations in 38 protected natural areas of Serbia. A total of 31 spider mite species were found, 10 from Bryobiinae and 21 from Tetranychinae. The species Eotetranychus fagi Zacher was recorded as new to Serbia and this record was also the first one for Southeast Europe. Spider mites were found on host plants in five basic types as well as many subtypes of terrestrial habitats, with woodland as the most dominant one. A total of 151 plant species from 44 families were recorded as hosts for spider mites including new world records: 60 new hosts for family Tetranychidae and 41 new hosts for 21 spider mite species. Host plants from Rosaceae family harbored the highest number of spider mite species (16). A considerable number of species was found on host plants from the families Betulaceae (11), Asteraceae (10) and Sapindaceae (10). Two cosmopolitan spider mites, Tetranychus urticae Koch and Tetranychus turkestani Ugarov & Nikolskii, were clearly distinguished with 67 (7 new) and 43 (13 new) recorded host species, respectively; among newly recorded hosts for Tetranychidae family, these two mite species were found on 27 and 12 hosts, respectively. After the two most common species, the most striking was the presence of Bryobia praetiosa Koch with 24 (4 new) recorded host plants, followed by Amphitetranychus viennensis (Zacher), Eotetranychus carpini (Oudemans) and Bryobia rubrioculus (Scheuten), with 21 (7 new), 20 (6 new) and 16 (2 new) hosts, respectively. The remaining tetranychids were found on 1–9 host plant species. This study provided the first insight into diversity of tetranychids in Serbian protected areas. Further research in this field should focus on mites from host plants representative of specific areas and habitats, including endangered, endemic and relict species.

scholarly journals Diversity and Pathogenicity of Phytophthora Species Associated with Declining Alder Trees in Italy and Description of Phytophthora alpina sp. nov

Forests ◽  
2020 ◽  
Vol 11 (8) ◽  
pp. 848
Author(s):  
Carlo Bregant ◽  
Gian Paolo Sanna ◽  
Adriano Bottos ◽  
Lucia Maddau ◽  
Lucio Montecchio ◽  
...  
Keyword(s):  
Rhizosphere Soil ◽  
Sequence Data ◽  
Single Agent ◽  
Phytophthora Species ◽  
Multiple Infections ◽  
Riparian Ecosystems ◽  
Dna Sequence Data ◽  
Alpine Regions ◽  
Green Alder ◽  
Depth Study

Extensive decline and mortality events of alder trees have recently been observed in several riparian ecosystems in Italy. Since there is little information about the aetiology of this disease and given the high ecological relevance of riparian ecosystems, an in-depth study was conducted in three sites spanning from the Mediterranean to Alpine regions. From spring 2019 to spring 2020, 261 samples of bleeding cankers, rhizosphere soil and leaves used as baits along waterways were collected and used for Phytophthora isolation. Based on morphology, colony appearance and DNA sequence data, 10 species belonging to 6 clades were identified. These included P. plurivora (84 isolates), P. pseudocryptogea (50), P. hydropathica (18), P. gonapodyides (14), P. bilorbang (13), P. pseudosyringae (12), P. lacustris (11), P. acerina (7), P. cactorum (1) and one isolate of the hybrid Phytophthora ×serendipita. In addition, two new Phytophthora species, one of which is described here as Phytophthora alpina sp. nov., were isolated. The pathogenicity of P. alpina and other species obtained from samples collected in the green alder stand was assessed on 3-year-old seedlings. All species proved to be pathogenic on green alder causing symptoms congruent with field observations. Results obtained have allowed us to expand knowledge about alder decline aetiology. The diversity of pathogenicity of Phytophthora species associated with symptomatic alder trees suggested that no single agent is responsible for the disease, but that it is the result of multiple infections of different Phytophthora species, variable in assemblages among sites.

scholarly journals Bud and Root Rot of Windmill Palm (Trachycarpus fortunei) Caused by Simultaneous Infections of Phytophthora palmivora and P. nicotianae in Sicily

Plant Disease ◽  
2011 ◽  
Vol 95 (6) ◽  
pp. 769-769 ◽  
Author(s):  
S. O. Cacciola ◽  
A. Pane ◽  
R. Faedda ◽  
C. Rizza ◽  
F. Badalà ◽  
...  
Keyword(s):  
Root Rot ◽  
Rhizosphere Soil ◽  
Sprinkler Irrigation ◽  
Its Region ◽  
Selective Medium ◽  
Phytophthora Species ◽  
Phytophthora Palmivora ◽  
Infested Soil ◽  
Tissue Samples ◽  
Reference Isolate

In June 2009 in a commercial nursery in eastern Sicily (Italy), 3-year-old potted windmill palms (Trachycarpus fortunei (Hooker) H. Wendl.) showed a decline in growth, wilt, droop, and basal rot of the youngest leaves. The rot progressed inward and killed the bud. Initially, older leaves remained green but eventually the entire plant collapsed. Root rot was consistently associated with aboveground symptoms. Two Phytophthora species were consistently isolated from the petiole base, heart, roots, and rhizosphere soil of symptomatic plants on a selective medium (2) and occasionally recovered from roots and rhizosphere soil of asymptomatic plants. Pure cultures were obtained by single-hypha transfers and the two species were identified on the basis of morphological and molecular characters as Phytophthora palmivora and P. nicotianae. Both species were recovered from all symptomatic plants. From multiple tissue samples per plant, we recovered either or both species. On potato dextrose agar (PDA), P. palmivora isolates grew between 10 and 35°C, with the optimum at 27°C. On V8 juice agar, they produced elliptical to ovoid, papillate, caducous sporangia (32 to 78 × 23 to 39 μm) with a mean length/breadth (l/b) ratio of 1.8:1 and a short pedicel (mean pedicel length = 5 μm). Isolates of P. nicotianae produced arachnoid colonies on PDA, grew at 37°C but did not grow at 40°C. Sporangia (29 to 55 × 23 to 45 μm) were spherical to ovoid (l/b ratio 1.3:1), papillate and often bipapillate, and noncaducous. Isolates of both species produced amphigynous antheridia and oogonia only when paired with reference isolates of P. nicotianae of the A2 mating type. The internal transcribed spacer (ITS) region of rDNA of two isolates of P. palmivora (IMI 398987 and IMI 398988) and an isolate of P. nicotianae (IMI 398989) from T. fortunei was amplified with primers ITS6/ITS4 and sequenced (1). Blast analysis of the sequences of isolates IMI 398987 and IMI 398988 (GenBank Accession Nos. HQ596556 and HQ596558) showed 99% homology with the sequence of two reference isolates of P. palmivora (GQ398157.1 and GU258862), while the sequence of isolate IMI 398989 (HQ596557) showed 99% homology with a reference isolate of P. nicotianae (EU331089.1). Pathogenicity of isolates IMI 398987 and IMI 398989 was proved by inoculating separately each isolate on 1-year-old potted plants of T. fortunei (10 plants per isolate). A zoospores suspension (2 × 104 zoospores/ml) was pipetted onto the petiole base of the three central leaves (200 μl per leaf) of each plant. Sterile water was used for control plants. All plants were incubated at 25 ± 2°C with 100% humidity for 48 h and then maintained in a greenhouse at 24 to 28°C. Within 3 weeks, all inoculated plants showed symptoms of bud rot. Control plants remained healthy. P. palmivora and P. nicotianae were reisolated only from inoculated plants. Bud rot of palms caused by P. palmivora was reported previously in Italy (3). However, to our knowledge, this is the first report of simultaneous infections of P. palmivora and P. nicotianae as causal agents of this disease. Outbreak of bud rot may have been favored by overhead sprinkler irrigation. The recovery of P. palmivora and P. nicotianae from rhizosphere soil and roots of asymptomatic plants suggests infested soil was the primary inoculum source. References: (1) D. E. L. Cooke et al. Fungal Genet. Biol. 30:17, 2000. (2) H. Masago et al. Phytopathology 67:425, 1977. (3) A. Pane et al. Plant Dis. 91:1059, 2007.

Protected Natural Areas of Puerto Rico

2011 ◽  
Author(s):  
William A. Gould ◽  
Maya Quinones ◽  
Mariano Solorzano ◽  
Waldemar Alcobas ◽  
Caryl Alarcon
Keyword(s):  
Puerto Rico ◽  
Natural Areas ◽  
Protected Natural Areas

(A review) Atlas of especially protected natural areas of Saint Petersburg / Ed. in chief V. N. Khramtsov, T. V. Kovaleva, N. Yu. Natsvaladze. St. Petersburg, 2013. 176 p.

2014 ◽  
pp. 124-129
Author(s):  
Z. V. Karamysheva
Keyword(s):  
Protected Areas ◽  
Large Scale ◽  
Natural Areas ◽  
Natural Protected Areas ◽  
Saint Petersburg ◽  
Natural Processes ◽  
Original Illustrations ◽  
Vegetation Maps ◽  
Protected Natural Areas

The review contains detailed description of the «Atlas of especially protected natural areas of Saint Petersburg» published in 2013. This publication presents the results of long-term studies of 12 natural protected areas made by a large research team in the years from 2002 to 2013 (see References). The Atlas contains a large number of the historical maps, new satellite images, the original illustrations, detailed texts on the nature of protected areas, summary tables of rare species of vascular plants, fungi and vertebrates recorded in these areas. Special attention is paid to the principles of thematic large-scale mapping. The landscape maps, the vegetation maps as well as the maps of natural processes in landscapes are included. Reviewed Atlas deserves the highest praise.

The International Legal Status of Marshes of Iraq as Protected Natural Areas

2019 ◽  
Vol 8 (4) ◽  
Author(s):  
Salwan Jaber Hashim ◽  
Valiullina Ksenia Borisovna ◽  
Davletgildeev Rustem Schamilevich
Keyword(s):  
Legal Status ◽  
Natural Areas ◽  
Protected Natural Areas

Topical issues of cartographic supporting specially protected natural areas of Russia

2019 ◽  
Vol 943 (1) ◽  
pp. 13-23
Author(s):  
N.A. Alekseenko
Keyword(s):  
Protected Areas ◽  
Annual Reports ◽  
Natural Areas ◽  
Specially Protected Natural Areas ◽  
Protected Natural Areas

In protected areas of Russia unique spatial-coordinated data on their territories on certain positions and methods is collected by local and other scientists. The data is stored in various formats (sometimes physically lost), very rarely in the form of maps, some of them in the annual reports are transferred to the MNR. Systematically arranged collecting, storage, analysis and transfer of these data could be significantly enhanced and optimized

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