Outbreak of Mortality Associated with Acipenser Iridovirus European (AcIV-E) Detection in Siberian Sturgeon (Acipenser baerii) Farmed in Sweden

Infectious disease is a major challenge in aquaculture and poses a constraint for the development of farming of new species. In 2017, Siberian sturgeon (Acipenser baerii) juveniles were imported from Italy to a Swedish farm. Transport conditions were suboptimal. Thirty percent died during transport and within the first days after arrival. Ten days after arrival, mortalities started to occur again, which prompted initiation of an investigation into the mortalities. Diseased fish were transported live to the National Veterinary Institute (SVA) for necropsy and further analysis. Pathological and histopathological investigation was conducted. Virology was performed on gills and internal organs by cell culture isolation and using specific PCR protocols against nervous necrosis virus (NNV) and Acipenser iridovirus European (AcIV-E). The juveniles displayed neurological signs such as lethargy, inability to maintain an upright position, and erratic swimming. Body condition was below normal, and gills were pale. One fish had petechial hemorrhages on the abdomen and the snout. Two specimens had intestinal hyperemia. Ventricles were air-filled, and swim bladders were deflated. Viral cell cultures gave negative results, but PCR analysis of gills and internal organs detected the presence of AcIV-E. We conclude that AcIV-E was associated with disease and high mortality in the sturgeon juveniles, and stress probably aggravated the course of the infection.

Download Full-text

Outbreak of Mortality Associated with Acipenser Iridovirus European (AcIV-E) Detection in Siberian Sturgeon (Acipenser Baerii) Farmed in Sweden

10.20944/preprints201809.0117.v1 ◽

2018 ◽

Author(s):

Charlotte Axén ◽

Niccolò Vendramin ◽

Anna Toffan

Keyword(s):

Siberian Sturgeon ◽

Internal Organs ◽

National Veterinary Institute ◽

Acipenser Baerii ◽

Specific Pcr ◽

Petechial Hemorrhage ◽

Diseased Fish ◽

Cell Culture Isolation ◽

Histopathological Investigation ◽

Veterinary Institute

Background: Infectious disease is a major challenge in aquaculture and poses a constraint for development of farming of new species. In 2017, Siberian sturgeon (Acipenser baerii) juveniles were imported from Italy to a Swedish farm. Due to stressful conditions, 30% died during transport and in the first days after arrival. Ten days after arrival, mortalities started to occur again. Within two months, only 5% of the juveniles were still alive. Methods: Diseased fish were transported live to the National Veterinary Institute (SVA) for necropsy and further analysis. Pathological and histopathological investigation was conducted. Virology was performed on gills and internal organs by cell culture isolation and specific PCR protocols. Results: The juveniles displayed neurological signs such as lethargy, inability to maintain upright position and erratic swimming. Body condition was low. Gills were pale. One fish had petechial hemorrhage on the abdomen and the snout. The ventricles were air-filled with, but swim bladders were deflated. One specimen had intestinal hemorrhage. Viral cell cultures were negative, but PCR of gills and internal organs detected the presence of Acipenser Iridovirus European (AcIV-E). Conclusions: AcIV-E was associated with disease and high mortality in the sturgeon juveniles. Stress probably aggravated the course of the infection.

Download Full-text

CYP2C9 polymorphism in patients with epilepsy: genotypic frequency analyzes andphenytoin adverse reactions correlation

Arquivos de Neuro-Psiquiatria ◽

10.1590/s0004-282x2011000200002 ◽

2011 ◽

Vol 69 (2a) ◽

pp. 153-158 ◽

Cited By ~ 10

Author(s):

Carlos Alexandre Twardowschy ◽

Lineu César Werneck ◽

Rosana Herminia Scola ◽

Luciano De Paola ◽

Carlos Eduardo Silvado

Keyword(s):

Adverse Reactions ◽

Pcr Analysis ◽

Similar Distribution ◽

Genotypic Frequency ◽

Negative Results ◽

Specific Pcr ◽

Cyp2c9 Polymorphism ◽

Literature Evidence ◽

Allele Type ◽

The Mean

OBJECTIVE: CYP2C9 is a major enzyme in human drug metabolism and the polymorphism observed in the corresponding gene may affect therapeutic outcome during treatment. The distribution of variant CYP2C9 alleles and prevalence of phenytoin adverse reactions were hereby investigated in a population of patients diagnosed with epilepsy. METHOD: Allele-specific PCR analysis was carried out in order to determine frequencies of the two most common variant alleles, CYP2C9*2 and CYP2C9*3 in genomic DNA isolated from 100 epileptic patients. We also analyzed the frequency of phenytoin adverse reactions among those different genotypes groups. The data was presented as mean±standard deviation. RESULTS: The mean age at enrollment was 39.6±10.3 years (range, 17-72 years) and duration of epilepsy was 26.5±11.9 years (range 3-48 years). The mean age at epilepsy onset was 13.1±12.4 years (range, 1 month-62 years). Frequencies of CYP2C9*1 (84%), CYP2C9*2 (9%) and CYP2C9*3 (7%) were similar to other published reports. Phenytoin adverse reactions were usually mild and occurred in 15% patients, without correlation with the CYP2C9 polymorphism (p=0.34). CONCLUSION: Our findings indicate an overall similar distribution of the CYP2C9 alleles in a population of patients diagnosed with epilepsy in the South of Brazil, compared to other samples. This sample of phenytoin users showed no drug related adverse reactions and CYP2C9 allele type correlation. The role of CYP2C9 polymorphism influence on phenytoin adverse reaction remains to be determined since some literature evidence and our data found negative results.

Download Full-text

Impact of starvation on digestive enzymes activities and plasma metabolites in Siberian sturgeon ( Acipenser baerii , Brandt, 1869)

Aquaculture Research ◽

10.1111/are.14515 ◽

2020 ◽

Vol 51 (4) ◽

pp. 1689-1699

Author(s):

Sedigheh Babaei ◽

Abdolmohammad Abedian-Kenari ◽

Mahmood Naseri ◽

Mohammad Ali Yazdani-Sadati ◽

Isidoro Metón

Keyword(s):

Digestive Enzymes ◽

Siberian Sturgeon ◽

Plasma Metabolites ◽

Acipenser Baerii ◽

Enzymes Activities

Download Full-text

Genetic parameters of caviar yield, color, size and firmness using parentage assignment in an octoploid fish species, the Siberian sturgeon Acipenser baerii

Aquaculture ◽

10.1016/j.aquaculture.2021.736725 ◽

2021 ◽

pp. 736725

Author(s):

A. Bestin ◽

O. Brunel ◽

A. Malledant ◽

B. Debeuf ◽

P. Benoit ◽

...

Keyword(s):

Fish Species ◽

Genetic Parameters ◽

Siberian Sturgeon ◽

Parentage Assignment ◽

Acipenser Baerii

Download Full-text

Supplementation of Siberian sturgeon (Acipenser baerii) diet with barberry (Berberis vulgaris) fruit extract: Growth performance, hemato-biochemical parameters, digestive enzyme activity, and growth-related gene expression

Aquaculture ◽

10.1016/j.aquaculture.2021.736750 ◽

2021 ◽

pp. 736750

Author(s):

Farshad Ramezani ◽

Seyed Pezhman Hosseini Shekarabi ◽

Mehdi Shamsaei Mehrgan ◽

Farhad Foroudi ◽

Houman Rajabi Islami

Keyword(s):

Gene Expression ◽

Enzyme Activity ◽

Growth Performance ◽

Biochemical Parameters ◽

Digestive Enzyme ◽

Siberian Sturgeon ◽

Related Gene ◽

Fruit Extract ◽

Acipenser Baerii ◽

Berberis Vulgaris

Download Full-text

Chromosome Distribution of Highly Conserved Tandemly Arranged Repetitive DNAs in the Siberian Sturgeon (Acipenser baerii)

Genes ◽

10.3390/genes11111375 ◽

2020 ◽

Vol 11 (11) ◽

pp. 1375

Author(s):

Larisa S. Biltueva ◽

Dmitry Yu. Prokopov ◽

Svetlana A. Romanenko ◽

Elena A. Interesova ◽

Manfred Schartl ◽

...

Keyword(s):

Duplication Event ◽

Siberian Sturgeon ◽

Genome Duplication Event ◽

Acipenser Baerii ◽

Whole Genome Duplications ◽

Sturgeon Species ◽

Genome Duplications ◽

Size Groups ◽

Genomic Studies ◽

Syntenic Regions

Polyploid genomes present a challenge for cytogenetic and genomic studies, due to the high number of similar size chromosomes and the simultaneous presence of hardly distinguishable paralogous elements. The karyotype of the Siberian sturgeon (Acipenser baerii) contains around 250 chromosomes and is remarkable for the presence of paralogs from two rounds of whole-genome duplications (WGD). In this study, we applied the sterlet-derived acipenserid satDNA-based whole chromosome-specific probes to analyze the Siberian sturgeon karyotype. We demonstrate that the last genome duplication event in the Siberian sturgeon was accompanied by the simultaneous expansion of several repetitive DNA families. Some of the repetitive probes serve as good cytogenetic markers distinguishing paralogous chromosomes and detecting ancestral syntenic regions, which underwent fusions and fissions. The tendency of minisatellite specificity for chromosome size groups previously observed in the sterlet genome is also visible in the Siberian sturgeon. We provide an initial physical chromosome map of the Siberian sturgeon genome supported by molecular markers. The application of these data will facilitate genomic studies in other recent polyploid sturgeon species.

Download Full-text