scholarly journals Lactobacillus plantarum MA2 Ameliorates Methionine and Choline-Deficient Diet Induced Non-Alcoholic Fatty Liver Disease in Rats by Improving the Intestinal Microecology and Mucosal Barrier

Foods ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 3126
Author(s):  
Yanping Wang ◽  
Yang Zhang ◽  
Jingnan Yang ◽  
Haoran Li ◽  
Jinju Wang ◽  
...  
Keyword(s):  
Liver Disease ◽  
Fatty Liver ◽  
Lactobacillus Plantarum ◽  
Fatty Liver Disease ◽  
Mucosal Barrier ◽  
Intragastric Administration ◽  
Deficient Diet ◽  
Alcoholic Fatty Liver ◽  
Alcoholic Fatty Liver Disease ◽  
Lactobacillus Plantarum Ma2

Non-alcoholic fatty liver disease (NAFLD) has become a highly concerned health issue in modern society. Due to the attentions of probiotics in the prevention of NAFLD, it is necessary to further clarify their roles. In this study, the methionine and choline-deficient (MCD) diet induced NAFLD rats model were constructed and treated with strain L. plantarum MA2 by intragastric administration once a day at a dose of 1 × 108 cfu/g.bw. After 56 days of the therapeutic intervention, the lipid metabolism and the liver pathological damage of the NAFLD rats were significantly improved. The content of total cholesterol (TC) and total triglyceride (TG) in serum were significantly lower than that in the NAFLD group (p < 0.05). Meanwhile, the intestinal mucosal barrier and the structure of intestinal microbiota were also improved. The villi length and the expression of claudin-1 was significantly higher than that in the NAFLD group (p < 0.05). Then, by detecting the content of LPS in the serum and the LPS-TLR4 pathway in the liver, we can conclude that Lactobacillus plantarum MA2 could reduce the LPS by regulating the gut microecology, thereby inhibit the activation of LPS-TLR4 and it downstream inflammatory signaling pathways. Therefore, our studies on rats showed that L. plantarum MA2 has the potential application in the alleviation of NAFLD. Moreover, based on the application of the strain in food industry, this study is of great significance to the development of new therapeutic strategy for NAFLD.

scholarly journals S100A4 Gene is Crucial for Methionine-Choline-Deficient Diet-Induced Non-Alcoholic Fatty Liver Disease in Mice

2018 ◽  
Vol 59 (9) ◽  
pp. 1064 ◽  
Author(s):  
Yin-Hua Zhang ◽  
De-Qiang Ma ◽  
De-Ping Ding ◽  
Juan Li ◽  
Lin-Li Chen ◽  
...  
Keyword(s):  
Liver Disease ◽  
Fatty Liver ◽  
Fatty Liver Disease ◽  
Deficient Diet ◽  
Alcoholic Fatty Liver ◽  
Alcoholic Fatty Liver Disease

Hypoxia aggravates non-alcoholic fatty liver disease in presence of high fat choline deficient diet: A pilot study

Life Sciences ◽  
2020 ◽  
Vol 260 ◽  
pp. 118404
Author(s):  
Soumalya Sarkar ◽  
Sankarsan Bhattacharya ◽  
Md. Jahangir Alam ◽  
Rajni Yadav ◽  
Sanjay K. Banerjee
Keyword(s):  
Liver Disease ◽  
Pilot Study ◽  
Fatty Liver ◽  
Fatty Liver Disease ◽  
High Fat ◽  
Deficient Diet ◽  
Alcoholic Fatty Liver ◽  
Alcoholic Fatty Liver Disease

scholarly journals Gene Set Enrichment Analysis (GSEA) of Differentially Expressed Genes in Green Tea against Methionine-Choline Deficient Diet in High-Fat Patients in the Development of Non-Alcoholic Fatty Liver Disease (NAFLD)

2018 ◽  
Author(s):  
Sarbojoy Saha ◽  
Ishtiaque Ahammad ◽  
Shampa Barmon
Keyword(s):  
Liver Disease ◽  
Fatty Liver ◽  
Green Tea ◽  
Fatty Liver Disease ◽  
Enrichment Analysis ◽  
Differentially Expressed ◽  
High Fat ◽  
Deficient Diet ◽  
Alcoholic Fatty Liver ◽  
Alcoholic Fatty Liver Disease

The most common liver disorder nowadays is non-alcoholic fatty liver disease(NAFLD) and it is a progressive disease that rises in severity from steatosis to nonalcoholic steatohepatitis(NASH), fibrosis and cirrhosis to increase risk of developing hepatocellular carcinoma. It is a cause of great concern as there is an estimated seventy million Americans who are currently affected by NAFLD, and this is expected to only increase because of its association with obesity and diabetes and also a lack of therapies to keep its development and progression in check. In this particular study we performed a gene set enrichment analysis(GSEA) of differentially expressed genes in a green tea against methionine-choline deficient diet in high-fat patients in the development of non-alcoholic fatty liver disease(NAFLD). The downregulated genes were used to perform an enrichment analysis and in the ARCHS4 TFs Coexpression database the most significant gene was found to be KLF5_human_tf_ARCHS4_coexpression. In the ARCHS4 Kinases Coexpression pathway database STYK1_human_kinase_ARCHS4 Coexpression was found to be the most significant gene. And finally for the upregulated genes a similar enrichment analysis was performed and in the humancy database &gamma;-linolenate biosynthesis_Homo sapiens_PWY-6000 gene was discovered to be the most significant one. This study has used bioinformatics tools and the Enrichr software to perform a comparative analysis of differentially expressed gene sets for high-fat patients having a diet consisting of green tea against a methionine-choline deficient diet. Green tea is known to contain several antioxidants and polyphenols which provide protection against many liver diseases such as non-alcoholic fatty liver disease(NAFLD). The present study simply tries to build awareness of this to the general public and allow them to learn more about certain diets which have protective effects against liver diseases. Hopefully by implementing these in their daily lifestyles the public can gain some form of protection against these types of liver disorders.

scholarly journals Beneficial Effects of Lactobacillus plantarum Strains on Non-Alcoholic Fatty Liver Disease in High Fat/High Fructose Diet-Fed Rats

Nutrients ◽  
2020 ◽  
Vol 12 (2) ◽  
pp. 542 ◽  
Author(s):  
Eun-Jung Park ◽  
You-Suk Lee ◽  
Sung Min Kim ◽  
Gun-Seok Park ◽  
Yong Hyun Lee ◽  
...  
Keyword(s):  
Liver Disease ◽  
Fatty Liver ◽  
Lactobacillus Plantarum ◽  
Fatty Liver Disease ◽  
Fecal Microbiota ◽  
Antioxidant Enzyme Activities ◽  
High Fat ◽  
Alcoholic Fatty Liver ◽  
High Fructose ◽  
Alcoholic Fatty Liver Disease

Emerging evidence suggests that probiotics are beneficial in non-alcoholic fatty liver disease (NAFLD). This study aimed to explore the effects of two Lactobacillus plantarum strains, ATG-K2 and ATG-K6 (isolated from Korean fermented cabbage), in a rat model of high fat/high fructose (HF/HF) diet-induced NAFLD. Rats with NAFLD were randomized into four groups (HF/HF diet control, (HC); HF/HF diet with silymarin, (PC); HF/HF diet with ATG-K2, (K2); and HF/HF diet with ATG-K6, (K6)) with healthy rats on a normal diet serving as the negative control. After treatment, histopathological and biochemical analyses of the blood and liver tissue were conducted. In addition, fecal microbiota was analyzed using the MiSeq platform. Compared with HC rats, K2 and K6 rats experienced significantly lower body weight gain, displayed decreased hepatic lipid accumulation, had lower serum levels of aspartate aminotransferase and alanine aminotransferase, and showed increased antioxidant enzyme activities. Moreover, de novo lipogenesis-related genes were downregulated following K2 and K6 administration. The fecal microbiota of K2 and K6 rats contained a higher proportion of Bacteriodetes and a lower proportion of Fimicutes than that of HC rats. Taken together, our results suggest that L. plantarum strains ATG-K2 and ATG-K6 are potential therapeutic agents for NAFLD.

scholarly journals The Role of the Microbiome and Intestinal Mucosal Barrier in the Development and Progression of Non-Alcoholic Fatty Liver Disease

2020 ◽  
Vol 30 (5) ◽  
pp. 42-48
Author(s):  
T. S. Krolevets ◽  
M. A. Livzan ◽  
S. I. Mozgovoy
Keyword(s):  
Liver Disease ◽  
Fatty Liver ◽  
Fatty Liver Disease ◽  
Human Microbiome ◽  
Short Chain Fatty Acids ◽  
Mucosal Barrier ◽  
Alcoholic Fatty Liver ◽  
Intestinal Mucosal Barrier ◽  
Alcoholic Fatty Liver Disease

Aim. To review available data on the role of the microbiome and intestinal mucosal barrier in the development and progression of non-alcoholic fatty liver disease (NAFLD).Key points. The role of the human microbiome in the development and progression of NAFLD is associated with its effects on the risk factors (obesity, insulin resistance, type 2 diabetes), permeability of the intestinal barrier and absorption of such substances as short-chain fatty acids, bile acids, choline and endogenous ethanol. Liver fibrosis constitutes the leading factor determining the prognosis of patients in NAFLD, including cases associated with cardiovascular complications. Changes in the microbiome composition were demonstrated for various degrees of fibrosis in NAFLD.Conclusion. The results of modern studies confirm the formation of a new concept in the pathophysiology of NAFLD, which encourages the development of new therapeutic strategies.

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