scholarly journals Efficacy of Lactic Acid and Modified Atmosphere Packaging against Campylobacter jejuni on Chicken during Refrigerated Storage

Foods ◽  
2020 ◽  
Vol 9 (1) ◽  
pp. 109 ◽  
Author(s):  
Elena Gonzalez-Fandos ◽  
Naiara Maya ◽  
Alba Martínez-Laorden ◽  
Iratxe Perez-Arnedo

The present study was conducted to evaluate the combined effect of lactic acid washing and modified atmospheres packaging on the counts of Campylobacter jejuni on chicken legs stored at 4 °C. In experiment 1, inoculated chicken legs were washed with either 1% or 2% lactic acid solution for 5 min or distilled water (control). The treatment with 2% lactic acid reduced C. jejuni counts 1.42 log units after treatment (day 0). In experiment 2, inoculated samples were packaged under different conditions: air, 100%N2, vacuum, 20%CO2/80%N2, or 40%CO2/60%N2. C. jejuni counts were higher in samples packaged under vacuum or atmospheres containing CO2 than in air. In experiment 3, inoculated chicken legs were washed with a 2% lactic acid solution for 5 min or distilled water (control). Samples were packaged under different conditions: air, vacuum, 20%CO2/80%N2, or 40%CO2/60%N2. C. jejuni counts were lower in samples treated with lactic acid than in samples non-treated. However, C. jejuni counts were higher in chicken legs treated with lactic acid and packaged in modified atmospheres than in those treated and packaged in air. Immersion of chicken legs in a solution containing 2% lactic acid can reduce C. jejuni counts on fresh chicken packaged in modified atmosphere.

2021 ◽  
Vol 6 (2) ◽  
pp. 190
Author(s):  
Dewi Puspitasari ◽  
Nurah Tajjalia ◽  
Diana Wibowo ◽  
Agung Satria Wardhana

Background: Bioactive resin can release calcium ions when contact with solution media, even in acid condition. In the oral cavity, pH may change into acid condition due to the metabolic results of Streptococcus mutans. The bacteria metabolize carbohydrates into organic acids, one of which is lactic acid. Purpose: Analyze the effect of lactic acid solution and artificial saliva on the number of the release of calcium ions of bioactive resin. Methods: Forty-two specimens (diameter 15 mm x thickness 1 mm; n= 7/group fabricated with Activa™ Bioactive Restorative (Pulpdent). The specimens that meet the criteria were divided into 6 groups. The specimen was immersed for 1 and 7 days in the incubator at 37oC. The number of calcium ion release is measured using titration method. Results: Two Way Anova test and Post Hoc Bonferonni test showed there were significant differences among all group for lactic acid 1 day (4.040 ± 0.360) µg, artificial saliva 1 day (0.640 ± 0.338) µg, distilled water 1 day (1.040 ± 0.504) µg, lactic acid 7 days (5.400 ± 0.312), artificial saliva 7 days (1.640 ± 0.215) µg, distilled water 7 days (3.520± 0.356 µg). Conclusion: There was an influence of lactic acid and artificial saliva on the number of calcium ion releases of bioactive resin. Immersion of bioactive resin in the lactic acid solution increase the calcium ion releases and artificial saliva decrease the calcium ion release compared to distilled water. Keywords: artificial saliva, bioactive resin, calcium ion release, lactic acid


2010 ◽  
Vol 79 (9) ◽  
pp. S127-S132 ◽  
Author(s):  
Radka Hulánková ◽  
Gabriela Bořilová ◽  
Iva Steinhauserová

The aim of this study was to find whether low numbers of Salmonella in the presence of natural microflora will survive on the surface of chicken legs stored in 30% CO2/70% N2 and 20% CO2/80% O2. In four experiments, a total of 240 pieces of chicken leg were inoculated with a Salmonella Enteritidis, PT 8, wildtype strain resulting in initial concentrations of approximately 4 log, 2.5 log, 1.5 log and 0.5 log cells per piece and kept under selected modified atmospheres for 14 days. Counts of Salmonella were determined by the Most Probable Number method (MPN) at days 0, 3, 7, 10 and 14 of storage. No significant increase or decrease in Salmonella numbers was observed in the atmosphere of 20% CO2/80% O2. In the atmosphere of 30% CO2/70% N2 there was a significant decrease in cell numbers at days 10 and 14; however, this decrease was proved only in experiments with an initial Salmonella concentration of 4 log and 1.5 log cells per piece. We proved that even low numbers of S. Enteritidis in the presence of natural microflora survive well on the surface of poultry stored at 3 °C in both modified atmospheres we tested. In the case of temperature abuse even products with low initial numbers of Salmonella may constitute a health risk for consumers.


Animals ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 1818
Author(s):  
Elena Gonzalez-Fandos ◽  
Alba Martinez-Laorden ◽  
Iratxe Perez-Arnedo

The combined effect of organic acid (citric, propionic or acetic acid) treatment and modified atmosphere packaging (MAP) on the growth of L. monocytogenes in chicken legs kept at 4 °C for 10 days was evaluated. Chicken legs were inoculated with L. monocytogenes and washed with either 2% citric, 2% propionic or 2% acetic acid solution or distilled water (control). Legs were packaged under the following conditions: air, vacuum, 80% N2/20% CO2, 60% N2/40% CO2 or 40% N2/60% CO2. The greatest L. monocytogenes growth reductions after treatment were observed in chicken legs washed with propionic acid (2.14 log units lower compared to control legs). The lowest growth rates of L. monocytogenes were found in samples washed with acetic acid and packaged in atmospheres containing CO2. An extended shelf life was observed in legs packaged in 40% N2/60% CO2, but these packaging conditions did not reduce L. monocytogenes growth. Consequently, it is necessary to design measures in order to control this bacterial pathogen. Washing of chicken with 2% propionic acid or 2% acetic acid can decrease L. monocytogenes counts in chicken packaged in MAP.


2021 ◽  
pp. 174751982110210
Author(s):  
Xiaolong Xu ◽  
Lijuan Liu

Lactic acid is an important platform compound used as raw material for the production of lactide and polylactic acid. However, its concentration and composition distribution are not as simple as those of common compounds. In this work, the mass concentration distribution of highly concentrated lactic acid is determined by back titration. The components of highly concentrated lactic acid, crude lactide, and polymer after the reaction are analyzed by HPLC. Different concentrations of lactic acid solution were prepared for the synthesis of lactide and its content in the product was determined by 1H NMR analysis. We found that lactide is more easily produced from high-concentration lactic acid solution with which the condensed water is easier to release. Hence, the removal of condensed water is crucial to the formation of lactide, although it is not directly formed by esterification of two molecules of lactic acid.


1999 ◽  
Vol 15 (6) ◽  
pp. 413-416 ◽  
Author(s):  
J.W. Nicholson ◽  
B.J. Millar ◽  
B. Czarnecka ◽  
H. Limanowska-Shaw

2010 ◽  
Vol 141 ◽  
pp. S140-S146 ◽  
Author(s):  
Nada Smigic ◽  
Andreja Rajkovic ◽  
Dennis Sandris Nielsen ◽  
Nils Arneborg ◽  
Henrik Siegumfeldt ◽  
...  

1991 ◽  
Vol 54 (3) ◽  
pp. 194-199 ◽  
Author(s):  
RANDALL K. PHEBUS ◽  
FRANCES A. DRAUGHON ◽  
JOHN R. MOUNT

Survival of Campylobacter jejuni, inoculated into turkey roll slices and stored under seven different atmospheric mixtures, was determined. Turkey roll samples were stored at 4°C for 18 d and at 21°C for 48 h. The effects of various atmospheric mixtures on aerobic, psychrotrophic, and lactic acid bacterial populations were also determined throughout storage. Campylobacter jejuni was inactivated under all atmospheric gas mixtures tested throughout storage. Increasing CO2 concentration inside the package from 0% to 100% CO2 resulted in a lower rate of inactivation of C. jejuni at both storage temperatures. Increases in CO2 concentrations provided greater inhibition of aerobic and psychrotrophic populations as compared to low CO2 levels. The effect of CO2 on survival of C. jejuni and growth rate of aerobic, psychrotrophic, and lactic acid bacteria was more pronounced at 4°C. Campylobacters were isolated from inoculated turkey roll held under all atmospheres by enrichment procedures on the 18th day and 48th hour of storage at 4 and 21°C, respectively, with an initial population of log 6.0 Campylobacter s/g. However, no Campylobacters were isolated by 18 d of storage at 4°C by direct plating.


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