As a promising biofabrication technology, extrusion-based bioprinting has gained significant attention in the last decade and major advances have been made in the development of bioinks. However suitable synthetic and stimuli-responsive bioinks are underrepresented in this context. <a>In this work, we described a hybrid system of nanoclay Laponite XLG and thermoresponsive block copolymer poly(2-methyl-2-oxazoline)-<i>b</i>-poly(2-<i>n</i>-propyl-2-oxazine) (PMeOx-<i>b</i>-PnPrOzi) as a novel biomaterial ink, and discussed its critical properties relevant for extrusion-based bioprinting, including viscoelastic properties and printability.</a> <a>The hybrid hydrogel retains the thermogelling properties but is strengthened by the added clay (over 5 kPa of storage modulus, and 240 Pa of yield stress). Importantly, the shear-thinning character is further enhanced, which, in combination with very rapid viscosity recovery (~1 s) and structure recovery (~10 s) is highly beneficial for extrusion-based 3D printing. Accordingly, various 3D patterns could be printed with markedly enhanced resolution and shape fidelity compared to the biomaterial ink without added clay.</a>
Precise Control over the Rheological Behavior of Associating Stimuli-Responsive Block Copolymer Gels
