Associations of Polymorphisms Localized in the 3′UTR Regions of the KRAS, NRAS, MAPK1 Genes with Laryngeal Squamous Cell Carcinoma

Background: Genetic variations, localized in the 3′ untranslated region (UTR) in mitogen-activated protein kinase (MAPK) pathway-related genes, may alter the transcription and impact the pathogenesis of laryngeal squamous cell carcinoma (LSCC). The present study investigated the associations of single-nucleotide polymorphisms (SNP), localized in the 3′UTR) of the KRAS, NRAS, and MAPK1 genes with LSCC risk and clinicopathological features. Methods: Genomic DNA and clinical data were collected from 327 adult men with LSCC. The control group was formed from 333 healthy men. Genotyping of the SNPs was performed using TaqMan SNP genotyping assays. Five KRAS, NRAS, and MAPK1 polymorphisms were analyzed. All studied genotypes were in Hardy–Weinberg equilibrium and had the same allele distribution as the 1000 Genomes project Phase 3 dataset for the European population. Results: Significant associations of the studied SNPs with reduced LSCC risk were observed between NRAS rs14804 major genotype CC. Significant associations of the studied SNPs with clinicopathologic variables were also observed between NRAS rs14804 minor T allele and advanced tumor stage and positive lymph node status. SNP of MAPK1 rs9340 was associated with distant metastasis. Moreover, haplotype analysis of two KRAS SNPs rs712 and rs7973450 revealed that TG haplotype was associated with positive lymph node status in LSCC patients. Conclusions: According to the present study, 3′UTR SNP in the NRAS and MAPK1 genes may contribute to the identifications of patients at higher risk of LSCC lymph node and distant metastasis development.

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Tumor budding score predicts lymph node status in oral tongue squamous cell carcinoma and should be included in the pathology report

PLoS ONE ◽

10.1371/journal.pone.0239783 ◽

2020 ◽

Vol 15 (9) ◽

pp. e0239783

Author(s):

Inger-Heidi Bjerkli ◽

Helene Laurvik ◽

Elisabeth Sivy Nginamau ◽

Tine M. Søland ◽

Daniela Costea ◽

...

Keyword(s):

Squamous Cell Carcinoma ◽

Lymph Node ◽

Cell Carcinoma ◽

Squamous Cell ◽

Tongue Squamous Cell Carcinoma ◽

Tumor Budding ◽

Lymph Node Status ◽

Pathology Report ◽

Oral Tongue ◽

Node Status

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Value of Positive Lymph Node Ratio for Predicting Postoperative Distant Metastasis and Prognosis in Esophageal Squamous Cell Carcinoma

Oncology Research and Treatment ◽

10.1159/000439038 ◽

2015 ◽

Vol 38 (9) ◽

pp. 424-428 ◽

Cited By ~ 3

Author(s):

Jia Huang ◽

Wenhao Hu ◽

Linrong Pang ◽

Jun Chen ◽

Hui Yang

Keyword(s):

Squamous Cell Carcinoma ◽

Lymph Node ◽

Esophageal Squamous Cell Carcinoma ◽

Cell Carcinoma ◽

Squamous Cell ◽

Distant Metastasis ◽

Lymph Node Ratio ◽

Positive Lymph Node ◽

Node Ratio

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Predictive Value of PET CT in the Histologic Lymph Node Status of Patients With Squamous Cell Carcinoma of the Oral N0 Clinical and Radiological Cavity.

Case Medical Research ◽

10.31525/ct1-nct03947892 ◽

2019 ◽

Author(s):

Keyword(s):

Squamous Cell Carcinoma ◽

Lymph Node ◽

Cell Carcinoma ◽

Squamous Cell ◽

Predictive Value ◽

Lymph Node Status ◽

Node Status ◽

Pet Ct

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Clinical significance of preoperative imaging in oral squamous cell carcinoma compared with lymph node status: a comparative retrospective study

Oral Surgery Oral Medicine Oral Pathology and Oral Radiology ◽

10.1016/j.oooo.2017.11.021 ◽

2018 ◽

Vol 125 (5) ◽

pp. 423-430 ◽

Cited By ~ 1

Author(s):

Elias Mazzawi ◽

Imad Abu El-naaj ◽

Yasmine Ghantous ◽

Salim Balan ◽

Edmond Sabo ◽

...

Keyword(s):

Squamous Cell Carcinoma ◽

Lymph Node ◽

Retrospective Study ◽

Oral Squamous Cell Carcinoma ◽

Cell Carcinoma ◽

Clinical Significance ◽

Squamous Cell ◽

Preoperative Imaging ◽

Lymph Node Status ◽

Node Status

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Evaluation and Correlation of Clinicopathological Parameters of Oral Squamous Cell Carcinoma of Gingivobuccal Sulcus with Lymph Node Status - A Retrospective Institutional Analysis in Navi Mumbai

Journal of Evolution of Medical and Dental Sciences ◽

10.14260/jemds/2021/469 ◽

2021 ◽

Vol 10 (30) ◽

pp. 2294-2299

Author(s):

Akash Shegaonkar ◽

Shilpa Patel ◽

Niharika Swain ◽

Jigna Pathak ◽

Rashmi Hosalkar ◽

...

Keyword(s):

Squamous Cell Carcinoma ◽

Lymph Node ◽

Oral Squamous Cell Carcinoma ◽

Cell Carcinoma ◽

Squamous Cell ◽

Perineural Invasion ◽

Tumour Size ◽

Clinicopathological Parameters ◽

Lymph Node Status ◽

Node Status

BACKGROUND Mortality and Morbidity rates in the patients diagnosed with oral cancers remain static despite availability of advanced diagnostic and treatment modalities. For improving the survival status of the patients, a thorough understanding of the factors that predicts the progression of oral cancer is necessary to determine appropriate line of treatment. To do so in practise, critical knowledge regarding the prognostic factors that has high sensitivity holds immense importance. For determination of prognosis in oral cancer patients, clinical and histopathological parameters are widely used for assessment of treatment strategies. The primary objective of this study was to determine the clinical and histopathological prognostic factors in patients of oral squamous cell carcinoma of gingivobuccal sulcus (GBSSCC) treated by surgical intervention (neck dissection). METHODS Histopathological evaluation of archived samples of 60 GBSSCC patients which were treated by surgical intervention (Neck Dissection) in the time period from January 2011 to December 2020. Recurrent cases were excluded. Clinicopathological parameters such as age, sex, habit, tumour site, tumour size, tumour differentiation, depth of invasion, bone invasion, muscle invasion, perineural invasion & extracapsular spread were evaluated & then correlated with lymphnode status. RESULTS Among all the parameters, variables like habit (tobacco use) (P = 0.045), tumour size (P = 0.003), perineural invasion (P = 0.000) emerged as independent prognosticators and significantly correlated to the lymph node status of the patients. CONCLUSIONS This analysis suggests that habit, tumour size, perineural invasion to be consistent, easy to assess and reliable independent prognosticators which are significantly correlated to the lymph node status. To conclude, it is of paramount importance to include the aforementioned prognosticators in histopathological reports for the prediction of clinical outcome and archiving of valued data for future analysis. KEY WORDS Oral Squamous Cell Carcinoma, Gingivobuccal sulcus, Lymph Node Status, ClinicoPathological Prognosticators

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p16INK4a expression and survival outcomes in patients with penile squamous cell carcinoma: The M.D. Anderson Cancer Center Experience.

Journal of Clinical Oncology ◽

10.1200/jco.2020.38.6_suppl.5 ◽

2020 ◽

Vol 38 (6_suppl) ◽

pp. 5-5 ◽

Cited By ~ 1

Author(s):

Jad Chahoud ◽

Rachel Pham ◽

Ming Guo ◽

Curtis R. Pickering ◽

Wei Qiao ◽

...

Keyword(s):

Squamous Cell Carcinoma ◽

Lymph Node ◽

Cell Carcinoma ◽

Squamous Cell ◽

Lymph Node Status ◽

Survival Outcomes ◽

Risk Of Death ◽

Node Status ◽

Hpv Status ◽

Penile Squamous Cell Carcinoma

5 Background: Penile Squamous Cell Carcinoma (PSCC) is associated with high risk human papillomavirus (HR-HPV) in about 50% of cases. The immunohistochemical test for p16INK4a (p16) is highly correlated with HR-HPV expression and used as prognostic marker for squamous cell carcinomas in various sites. The prognostic role of this marker in PSCC remains unclear. We studied whether the expression of HPV or p16INK4a is associated with survival in a large PSCC cohort. Methods: We conducted a single institution analysis of PSCC patients who received treatment between 1991-2017.Patients with a confirmed diagnosis of PSCC and available tissue were tested for HR-HPV status using the Cobas PCR assay. Histological subtype, tumor grade, LVI and p16 staining patterns were confirmed by an experienced pathologist. Patient characteristics were summarized using descriptive statistics of clinico-pathologic variables. Kaplan-Meier was used to estimate median overall survival (OS) and cancer specific survival (CSS). Log rank test, univariate and multivariate Cox models were applied to identify the prognostic factors for survival. Results: We identified 147 patients with PSCC, with available tissue for testing. The median follow-up was 5.2 years (95% CI; 4.48, 6.68y). Patients with p16(+) tumors showed a significantly longer median OS and CSS in comparison to the p16(–) group (p=0.038 and p=0.012), with respective 5 year OS probability of 73% (95% CI; 0.74, 0.98) in comparison to 56% (95% CI; 0.46, 0.67) and 5 year CSS probability of 89% (95% CI; 0.7, 1) in comparison to 64% (95% CI; 0.54, 0.75). In contrast, HPV status by PCR did not predict survival outcomes, with 5 year CSS probability for HPV(+) of 75% (95% CI; 0.61, 0.91) compared to 65% (95% CI; 0.55, 0.78) for HPV(–) patients. Multivariable analysis to evaluate the association with CSS, showed that p16(+) along with lymph node status was associated with lower risk of death (HR=0.28, 95%CI; 0.09-0.8, p=0.002), and OS (HR=0.49, 95%CI; 0.19-1.24, p=0.13) after adjusting for the covariates. Conclusions: Tumor p16 status was an independent prognostic factor for CSS in our PSCC cohort providing unique information above that of lymph node status alone.

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