Seed Micromorphology, In Vitro Germination, and Early-Stage Seedling Morphological Traits of Cattleya purpurata (Lindl. & Paxton) Van den Berg

In the context of a symbiotic plant-fungus interaction study concerning Cattleya purpurata, we focused on some aspects of seed morphology and biology, and the early stages of seedling development. Seed morphology was characterized using light and scanning electron microscopy. In vitro seed germination capability was evaluated, comparing symbiotic and asymbiotic methods. The morphology of the seeds was overall comparable to that of other congeneric species, showing classical adaptations related to the aerodynamic properties and to the wettability of seeds, but calcium oxalate druses were identified inside the suspensor cells. Asymbiotic seed germination was successful in all tested media (17.1–46.5%) but was higher on 1/2 Murashige & Skoog. During symbiotic interaction with the fungal strain MUT4178 (Tulasnella calospora), germination rate was significantly lower than that obtained with the best three asymbiotic media, suggesting a low fungal compatibility. Seedling morphology was in line with other taxa from the same genus, showing typical characteristics of epiphytic species. Our observations, in particular, highlighted the presence of stomata with C-shaped guard cells in the leaves, rarely found in Cattleyas (where usually they are reniform), and confirm the presence of tilosomes in the roots. Idioblasts containing raphides were observed in both roots and leaves.

Download Full-text

ASYMBIOTIC SEED GERMINATION AND PLANTLET DEVELOPMENT OF Dendrobium spectabile (Blume) Miq.

Buletin Kebun Raya ◽

10.14203/bkr.v23i1.3 ◽

2020 ◽

Vol 23 (1) ◽

pp. 25-35

Author(s):

Eka Martha Della Rahayu ◽

Melza Mulyani

Keyword(s):

Seed Germination ◽

Leaf Growth ◽

Germination Rate ◽

Economic Value ◽

Sphagnum Moss ◽

Tree Fern ◽

Asymbiotic Seed Germination ◽

Optimal Protocol ◽

Half Strength

Dendrobium spectabile (Blume) Miq. is one of Papuan orchids that is of economic value and threatened. At present, the optimal protocol for asymbiotic seed germination and planlet development of D. spectabile is not yet available. This research aimed to develop an optimal and comprehensive protocol for in vitro culture of D. spectabile to support the conservation and reintroduction of this species. The experiment was conducted using a completely random design, including seed sowing, protocorm subculture (subculture 1), plantlet subculture (subculture 2), and plantlet acclimatization. The highest germination rate at 3 MAS (months after sowing) was found in modified Knudson's C (92.59%). The first subculture at 4 MAP (months after planting) showed that the highest leaf and root growths were found in modified Vacin and Went (4.12 and 2.13, respectively). The second subculture at 6 MAP showed that the highest leaf growth was found in full strength of Murashige and Skoog supplemented with 100 g/l banana homogenate (5.49), while the highest number of roots and average root extension were found in half strength of Vacin and Went supplemented with 100 g/l banana homogenate (7.05 and 0.47 cm, respectively). The plantlets were best acclimatized in media consisting of tree fern fibre and sphagnum moss (2:1).

Download Full-text

In-Vitro Seed Germination and Effect of Growth Regulators on Subsequent Development of Protocorms of Eulophia Nuda Lindl

International Journal of Applied Sciences and Biotechnology ◽

10.3126/ijasbt.v3i2.12476 ◽

2015 ◽

Vol 3 (2) ◽

pp. 243-247 ◽

Cited By ~ 2

Author(s):

Varsha Dawande ◽

Rajaram Gurav

Keyword(s):

Seed Germination ◽

Root Growth ◽

Growth Regulators ◽

Subsequent Development ◽

Ms Medium ◽

Asymbiotic Seed Germination ◽

Endangered Orchid ◽

Maximum Root ◽

Regenerated Plantlets

Asymbiotic seed germination of Eulophia nuda Lindl. was observed on Knudson C medium. About 90% seeds germinated within 8-10 weeks and formed green protocorms in 11-12 weeks. Effect of BA and IBA was studied on plantlet development from protocorms. BA shows the best results with respect to number and length of shoots. Maximum number (6.45±1.36) and length (3.90±0.99) was observed on MS medium supplemented with 4.44μM.BA. Maximum root growth was also observed on same medium (4.8±0.99 number of roots and 1.43±0.13cm length). The regenerated plantlets were successfully acclimatized and transferred to earthen pots. The results presented here show that in vitro seed germination and plantlet development in Eulophia nuda Lindl., an endangered orchid, can be achieved at a higher rate by this method.Int J Appl Sci Biotechnol, Vol 3(2): 243-247 DOI: http://dx.doi.org/10.3126/ijasbt.v3i2.12476

Download Full-text

In vitro seed germination and seedling development of Phaius tancarvilleae (L’Her.) Blume.

Scientific World ◽

10.3126/sw.v9i9.5518 ◽

1970 ◽

Vol 9 (9) ◽

pp. 50-52 ◽

Cited By ~ 3

Author(s):

Bijaya Pant ◽

Sumitra Shrestha ◽

Shreeti Pradhan

Keyword(s):

Acetic Acid ◽

Seed Germination ◽

Seedling Development ◽

Naphthalene Acetic Acid ◽

Ideal Condition ◽

Ms Medium ◽

Mass Propagation ◽

Asymbiotic Seed Germination ◽

Protocorm Formation

In vitro seed germination and seedling development of Phaius tancarvilleae (L’Her.) Blume. was carried out on 0.8%(w/v) agar solidified MS Medium (Murashige and Skoog, 1962) without hormones or supplemented with different concentration and combination of Naphthalene acetic acid (NAA) and 6-benzylaminopurine (BAP). MS medium supplemented with 0.5 mg/l of BAP was the most ideal condition for early seed germination, protocorm formation and development of seedlings. Germination started after 7 weeks of culture and complete seedlings were obtained after 24 weeks of culture. This protocol might be helpful for mass propagation of orchids by asymbiotic seed germination. Keywords: Orchid; Invitro; Protocorm; Media DOI: http://dx.doi.org/10.3126/sw.v9i9.5518 SW 2011; 9(9): 50-52

Download Full-text

An effective nutrient medium for asymbiotic seed germination and large-scale in vitro regeneration of Dendrobium hookerianum, a threatened orchid of northeast India

AoB Plants ◽

10.1093/aobpla/plr032 ◽

2011 ◽

Vol 2012 ◽

Cited By ~ 12

Author(s):

Sumi Paul ◽

Suman Kumaria ◽

Pramod Tandon

Keyword(s):

Seed Germination ◽

Nutrient Medium ◽

Large Scale ◽

In Vitro Regeneration ◽

Northeast India ◽

Asymbiotic Seed Germination

Download Full-text

Establishment of an EfficientIn VitroRegeneration Protocol for Rapid and Mass Propagation ofDendrobium chrysotoxumLindl. Using Seed Culture

The Scientific World JOURNAL ◽

10.1155/2014/740150 ◽

2014 ◽

Vol 2014 ◽

pp. 1-8 ◽

Cited By ~ 9

Author(s):

Potshangbam Nongdam ◽

Leimapokpam Tikendra

Keyword(s):

Acetic Acid ◽

Seed Germination ◽

Germination Percentage ◽

Naphthalene Acetic Acid ◽

Mass Propagation ◽

Asymbiotic Seed Germination ◽

Orchid Species ◽

Seed Culture ◽

Leaf Formation

An efficientin vitroregeneration protocol from seed culture has been established successfully forDendrobium chrysotoxum, an epiphytic orchid having tremendous ornamental and medicinal values. Seed germination response was encouraging in Mitra (M) medium enriched with different combinations of auxins and cytokinins. Medium supplemented with 0.4% activated charcoal (AC), 2 mg/L 6-benzyl amino purine (BAP), and 2 mg/L indole-3-acetic acid (IAA) produced best seed germination percentage in 2 weeks of culture. Incorporation of higher concentration of kinetin (KN) or BAP in combination with low auxin in medium induced pronounced shooting and leaf formation. Reduction in leaf development was evident when cytokinins exist singly in medium indicating synergistic effect of auxin and cytokinin in leaf induction. Presence of elevated level of indole-3-butyric acid (IBA) or 1-naphthalene acetic acid (NAA) with low cytokinin content in medium generated morein vitrorooting, though IBA was found to be more effective in rooting induction as compared to NAA. Thein vitroprotocol for asymbiotic seed germination developed from the present investigation can be used for rapid mass propagation of this highly importantDendrobiumorchid species.

Download Full-text

Embryo development in association with asymbiotic seed germination in vitro of Paphiopedilum armeniacum S. C. Chen et F. Y. Liu

Scientific Reports ◽

10.1038/srep16356 ◽

2015 ◽

Vol 5 (1) ◽

Cited By ~ 12

Author(s):

Yan-Yan Zhang ◽

Kun-Lin Wu ◽

Jian-Xia Zhang ◽

Ru-Fang Deng ◽

Jun Duan ◽

...

Keyword(s):

Seed Germination ◽

Embryo Development ◽

Asymbiotic Seed Germination

Download Full-text

The influence of light spectra, UV-A, and growth regulators on the in vitro seed germination of Senecio cineraria DC.

Revista CERES ◽

10.1590/s0034-737x2010000500002 ◽

2010 ◽

Vol 57 (5) ◽

pp. 576-580 ◽

Cited By ~ 1

Author(s):

Cristiane Pimentel Victório ◽

Nina Cláudia Barbosa da Silva ◽

Maria Apparecida Esquibel ◽

Alice Sato

Keyword(s):

Seed Germination ◽

Growth Regulators ◽

White Light ◽

Germination Rate ◽

Germination Percentage ◽

Ms Medium ◽

In Vitro Germination ◽

Light Spectra ◽

Indole 3 Acetic Acid

This study was carried out to investigate the effects of light spectra, additional UV-A, and different growth regulators on the in vitro germination of Senecio cineraria DC. Seeds were surface-sterilized and inoculated in MS medium to evaluate the following light spectra: white, white plus UV-A, blue, green, red or darkness. The maximum germinability was obtained using MS0 medium under white light (30%) and MS + 0.3 mg L-1 GA3 in the absence of light (30.5%). S. cineraria seeds were indifferent to light. Blue and green lights inhibited germination. Different concentrations of gibberellic acid (GA3) (0.1; 0.4; 0.6; 0.8; 1.0 and 2.0 mg L-1) and indole-3-acetic acid IAA (0.1; 0.3 and 1.0 mg L-1) were evaluated under white light and darkness. No concentration of GA3 enhanced seed germination percentage under white light. However, when the seeds were maintained in darkness, GA3 improved germination responses in all tested concentrations, except at 1.0 mg L-1. Under white light, these concentrations also increased the germination time and reduced germination rate. Germination rate, under light or darkness, was lower using IAA compared with GA3.

Download Full-text