scholarly journals Transcriptome Analysis Reveals Genes Respond to Chlorophyll Deficiency in Green and Yellow Leaves of Chrysanthemum morifolium Ramat

Horticulturae ◽  
2021 ◽  
Vol 8 (1) ◽  
pp. 14
Author(s):  
Gang Shao ◽  
Rui Liu ◽  
Ziyan Qian ◽  
Hua Zhang ◽  
Qian Hu ◽  
...  
Keyword(s):  
Leaf Size ◽  
Chrysanthemum Morifolium ◽  
Chlorophyll Deficiency ◽  
Stem Cell Maintenance ◽  
Physiological Processes ◽  
Chlorophyll Accumulation ◽  
Yellow Color ◽  
Bud Sport ◽  
Molecular Effects ◽  
Chrysanthemum Morifolium Ramat

Chlorophyll is vital for photosynthesis to produce sugars and other useful biochemical products in green plants. However, the molecular effects of chlorophyll deficiency in Chrysanthemum are largely unknown. In this study, we identified a bud sport mutant chrysanthemum belonging to the variety ‘Nannong Binyun’, which has yellow branches. Plant physiological studies have shown that the yellow color is revealed due to chlorophyll loss. RNA extracts of yellow and green tissues were analyzed using high-throughput RNA-sequencing, and a total of 11,649 tissue enriched unigenes that respond to chlorophyll deficiency were identified, including 4803 unigenes upregulated in yellow tissues and 6846 unigenes in green tissues. GO analysis revealed that these tissue-enriched genes may involve in the physiological processes of chlorophyll accumulation and photosynthesis. In addition, many DEGs from the families of AP2-EREBP, bHLH, MYB, and FAR1 that are associated with plant development and stress response were detected. Our study found that most of the genes from the GRAS family were downregulated in yellow leaves, indicating their putative roles in stem cell maintenance and possible contribution to leaf size determination.

scholarly journals Callus Induction of Leaves and Stems in Krisan (Chrysanthemum morifolium Ramat cv Dewi ratih) with Alternative Foliar Fertilizers Media

2021 ◽  
Vol 9 (2) ◽  
pp. 109-115
Author(s):  
Ahmad Saifun Naser ◽  
Muhammad Wisnu
Keyword(s):  
Tissue Culture ◽  
Callus Formation ◽  
Leaf Explants ◽  
Chrysanthemum Morifolium ◽  
Stem Explants ◽  
Appearance Time ◽  
Randomized Design ◽  
The Media ◽  
Completely Randomized Design ◽  
Chrysanthemum Morifolium Ramat

Availability of quality seeds in production of krisan (Chrysanthemum morifolium Ramat cv Dewi ratih) cultivation is still rare, therefore research on seed multiplication through tissue culture is needed. The media used in tissue culture is relatively expensive for home industry. This study aims to determine the respond of leaf and stem explants using foliar fertilizers (Growmore, Gandasil D and Mutiara) as an alternative media for callus inductions. This study used a Completely Randomized Design (CRD) consisted of 4 treatments: P0: ½ MS + 0,25 mg/l BAP, P1 (Growmore + 0,25 mg/l BAP), P2 (Gandasil D + 0,25 mg/l BAP), P3 (Mutiara + 0,25 mg/l BAP). The variables observed in this study included callus appearance time, callus color and callus texture. The result of this study indicated that the use of BAP (6-Benzyl Amino Purine) affected the time of callus formation and callus morphology. Callus was formed on leaf explants 13 days after planting while on stem explants 7 days after planting and compact texture. Growmore + 0,25 mg/l BAP treatment yields the best callus on leaf explant, while Gandasil D + 0,25 mg/l BAP treatment yields the best callus on stem explant.

scholarly journals Selection for wide temperature adaptation in Chrysanthemum morifolium (Ramat.) Hemsl.

1978 ◽  
Vol 26 (1) ◽  
pp. 110-118
Author(s):  
J. de Jong
Keyword(s):  
Low Temperature ◽  
Chrysanthemum Morifolium ◽  
Temperature Adaptation ◽  
Optimum Temperature ◽  
Selected Abstract ◽  
Days To Flowering ◽  
Selection For ◽  
High Or Low Temperature ◽  
Chrysanthemum Morifolium Ramat ◽  
Short Days

Rooted cuttings of commercial cvs were grown to flowering at five temperatures and the the number of short days to flowering was recorded. The optimum temperature for rapid flowering varied between cvs. The number of days to flowering at the optimum temperature was not related to the delay in flowering caused by either high or low temperature. In many cvs the delay in flowering at low temperature was accompanied by a similar delay at high temperature. It was concluded that for the character 'time to flowering' genotypes should preferably be selected at low temperatures. If low temperature cannot be realized, only rapidly flowering genotypes should be selected. (Abstract retrieved from CAB Abstracts by CABI’s permission)

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