Performance of Qualitative and Quantitative Antigen Tests for SARS-CoV-2 Using Saliva

The rapid detection of SARS-CoV-2 is critical for the prevention of disease outbreaks. Antigen tests such as immunochromatographic assay (ICA) and chemiluminescent enzyme immunoassay (CLEIA) can yield results more quickly than PCR. We evaluated the performance of ICA and CLEIA using 34 frozen PCR-positive (17 saliva samples and 17 nasopharyngeal swabs [NPS]) and 309 PCR-negative samples. ICA detected SARS-CoV-2 in only 14 (41%) samples, with positivity rates of 24% in saliva and 59% in NPS. Notably, ICA detected SARS-CoV-2 in 5 of 6 samples collected within 4 days after symptom onset. CLEIA detected SARS-CoV-2 in 31 (91%) samples, with a positivity of 82% in saliva and 100% in NPS. These results suggest that the use of ICA should be limited to an earlier time after symptom onset and CLEIA is more sensitive and can be used in situations where quick results are required.

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Performance of qualitative and quantitative antigen tests for SARS-CoV-2 in early symptomatic patients using saliva

10.1101/2020.11.06.20227363 ◽

2020 ◽

Author(s):

Isao Yokota ◽

Takayo Sakurazawa ◽

Junichi Sugita ◽

Sumio Iwasaki ◽

Keiko Yasuda ◽

...

Keyword(s):

Symptom Onset ◽

Gold Standard ◽

Rapid Detection ◽

Disease Outbreaks ◽

Nasopharyngeal Swab ◽

Chain Reaction ◽

Qualitative And Quantitative ◽

Polymerase Chain ◽

Antigen Tests ◽

Chemiluminescent Enzyme Immunoassay

AbstractBackgroundThe rapid detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an urgent need for the prevention and containment of disease outbreaks in communities. Although the gold standard is polymerase chain reaction (PCR), antigen tests such as immunochromatographic assay (ICA) and chemiluminescent enzyme immunoassay (CLEIA) that can yield results within 30 minutes.MethodsWe evaluated performance of ICA and CLEIA using 34 frozen PCR-positive specimens (17 saliva and 17 nasopharyngeal swab) and 307 PCR-negative samples.ResultsICA detected SARS-CoV-2 in only 14 (41%) samples, with positivity of 24% in saliva and 59% in NPS. Notably, ICA detected SARS-CoV-2 in 5 (83%) of 6 samples collected within 4 days after symptom onset. CLEIA detected SARS-CoV-2 in 31 (91%) samples, with positivity of 82% in saliva and 100% in NPS. CLEIA was negative in 3 samples with low viral load by PCR.ConclusionsThese results suggest that use of ICA should be limited to earlier time after symptom onset and CLEIA is more sensitive and can be used in situations where quick results are required.

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Chemiluminescent Enzyme Immunoassay for Rapid Detection of Three α-Cyano Pyrethroid Residues in Agricultural Products

Food Analytical Methods ◽

10.1007/s12161-016-0482-x ◽

2016 ◽

Vol 9 (10) ◽

pp. 2896-2905 ◽

Cited By ~ 2

Author(s):

Niusha Taheri ◽

Meijing Lan ◽

Peng Wei ◽

Rui Liu ◽

Wenjun Gui ◽

...

Keyword(s):

Enzyme Immunoassay ◽

Rapid Detection ◽

Agricultural Products ◽

Chemiluminescent Enzyme Immunoassay

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Immunochromatography and chemiluminescent enzyme immunoassay for COVID-19 diagnosis

Journal of Infection and Chemotherapy ◽

10.1016/j.jiac.2021.02.025 ◽

2021 ◽

Vol 27 (6) ◽

pp. 915-918 ◽

Cited By ~ 1

Author(s):

Toshiaki Ishii ◽

Masakazu Sasaki ◽

Kageto Yamada ◽

Daiki Kato ◽

Hiroyoshi Osuka ◽

...

Keyword(s):

Enzyme Immunoassay ◽

Chemiluminescent Enzyme Immunoassay

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Development of a monoclonal antibody-based immunochromatographic strip for the rapid detection of tigecycline in human serum

Analytical Methods ◽

10.1039/d0ay02182b ◽

2021 ◽

Author(s):

Chuanlai Xu ◽

qianqian lu ◽

Xin-Xin Xu ◽

Shanshan Song ◽

aihong wu ◽

...

Keyword(s):

Monoclonal Antibody ◽

Human Serum ◽

Rapid Detection ◽

Immunochromatographic Assay ◽

Immunochromatographic Strip

In this study, a gold labelled immunochromatographic assay was developed to detect tigecycline (TGC) in human serum. For this purpose, an anti-TGC monoclonal antibody, 2G7, was produced and characterized, and...

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