scholarly journals Anti-Blastocystis Activity In Vitro of Egyptian Herbal Extracts (Family: Asteraceae) with Emphasis on Artemisia judaica

Author(s):  
Amira B. Mokhtar ◽  
Shahira A. Ahmed ◽  
Enas E. Eltamany ◽  
Panagiotis Karanis

Achillea fragrantissima (Forssk.) Sch. Bip. (known as Qaysoom), Echinops spinosus L. (known as Shoak Elgamal) and Artemisia judaica L. (known Shih Baladi) are members of the Asteraceae family known for their traditional medical use in Egypt. The ethanol extracts of these plants were evaluated for their efficacy against a protozoan parasite (Blastocystis). Two different molecular subtypes of Blastocystis were used (ST1 and ST3). Significant growth inhibition of Blastocystis was observed when exposed to both A. judaica (99.3%) and A. fragrantissima (95.6%) with minimal inhibitory concentration (MIC90) at 2000 µg/mL. Under the effect of the extracts, changes in Blastocystis morphology were noted, with the complete destruction of Blastocystis forms after 72 h with the dose of 4000 µg/mL. Different subtypes displayed different responses to the herbal extracts tested. ST1 exhibited significantly different responses to the herbal extracts compared to ST3. A. judaica was selected as the herb of choice considering all of its variables and because of its effective action against Blastocystis. It was then exposed to further fractionation and observation of its effect on ST1 and ST3. Solvent portioned fractions (dichloromethane (DCM), ethyl acetate (EtOAc) and n-hexane) in A. judaica were found to be the potent active fractions against both of the Blastocystis subtypes used.

2020 ◽  
Vol 36 (6) ◽  
Author(s):  
Cleberton Torres Santos ◽  
Luiz Eduardo Oliveira Teotônio ◽  
Ana Paula Leite Nascimento ◽  
Darcio Luiz de Sousa Júnior ◽  
Ítalo Mykaell da Silva Benjamin ◽  
...  

Baccharis cinerea belongs to the Asteraceae family, in Brazil is found in the Northeast and Southeast, occurring in the Caatinga and Mata Atlântica biomes, on the edges of the seasonal forests, board and altitude forests in both regenerating primary and secondary areas. Has proven antimicrobial and antiviral activity and is widely used in folk medicine for its various therapeutic effects and is used as an antiseptic for skin and wound infections, inflammation, diarrhea as well as being used as a purgative. The plants used in the traditional medicine are more and more explored scientifically because they are possible resources of substances with antimicrobial activity in front damage man’s health microorganism. In this context the objective of the study was to investigate the antimicrobial activity, modulator activity of antibiotic and in vitro phytochemical prospection of leaf ethanol extracts. Tests were performed on the bacterial strains of Staphylococcus aureus (ATCC 25923), Pseudomonas aeruginosa (ATCC 15442) and Escherichia coli (ATCC 10536). The antibacterial activity was analyzed by means determining the Minimum Inhibitory Concentration (MIC). For the evaluation of the modulating activity, the microdilution method of the diluted extract samples with the antibiotic’s amikacin, clindamycin and gentamicin was used. The MIC results were ≥ 1024 μg mL-1 by the bacterial strains. There was a relevance of concentrations in modulation with the antimicrobials tested such as amikacin and gentamicin, there were no discrepancy of clindamycin results in association with the extract. The chemical constituents found were leucoanthocyanidins, flabobenic tannins, flavanones, flavones, flavonoids, xanthones, chalcones, aurones. It is important to note that is necessary to do other studies to evaluate the potential of this species because it has important chemical compounds in reducing antimicrobial resistance.


2017 ◽  
Vol 2017 ◽  
pp. 1-6 ◽  
Author(s):  
Renata Albuquerque Costa ◽  
Oscarina Viana de Sousa ◽  
Ernesto Hofer ◽  
Jair Mafezoli ◽  
Francisco Geraldo Barbosa ◽  
...  

Prospect of antibacterial agents may provide an alternative therapy for diseases caused by multidrug-resistant bacteria. This study aimed to evaluate the in vitro bioactivity of Moringa oleifera seed extracts against 100 vibrios isolated from the marine shrimp Litopenaeus vannamei. Ethanol extracts at low (MOS-E) and hot (MOS-ES) temperature are shown to be bioactive against 92% and 90% of the strains, respectively. The most efficient Minimum Inhibitory Concentration (MIC) levels of MOS-E and MOS-ES against a high percentage of strains were 32 µg mL−1. Bioguided screening of bioactive compounds showed that the ethyl acetate fraction from both extracts was the only one that showed antibacterial activity. Vibriocidal substances, niazirine and niazimicine, were isolated from the aforementioned fraction through chromatographic fractionation.


2011 ◽  
Vol 20 (3) ◽  
pp. 215-218 ◽  
Author(s):  
Silvana Krychak-Furtado ◽  
Ana Luisa Palhano Silva ◽  
Obdulio Gomes Miguel ◽  
Josiane de Fátima Gaspari Dias ◽  
Marilis Dallarmi Miguel ◽  
...  

Data on in vitro evaluation of extracts of three species of the Asteraceae family on the development of Trichostrongylidae eggs in sheep are presented. Egg hatchability was tested using herbal extracts prepared in a Soxhlet extractor, and using hydrolate prepared by means of hydrodistillation. The laboratory tests showed that the ethanol extract from flowers of the species Aster lanceolatus presented high activity against Trichostrongylidae eggs development in sheep, inhibiting larva formation by 91% within 48 hours, and maintaining similar rates after 72 hours.


Planta Medica ◽  
2010 ◽  
Vol 76 (12) ◽  
Author(s):  
C Tarirai ◽  
A Viljoen ◽  
P Chinyemba ◽  
A Khatib ◽  
P Jiyane ◽  
...  

Planta Medica ◽  
2011 ◽  
Vol 77 (12) ◽  
Author(s):  
D Sevim ◽  
FS Senol ◽  
I Orhan ◽  
B Şener ◽  
E Kaya

Planta Medica ◽  
2014 ◽  
Vol 80 (10) ◽  
Author(s):  
JP Jackson ◽  
K Freeman ◽  
J Hatfield ◽  
B St Claire ◽  
C Hubert ◽  
...  

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
EM Pferschy-Wenzig ◽  
K Koskinen ◽  
C Moissl-Eichinger ◽  
R Bauer

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