scholarly journals Effect of Sodium Fluoride Ingestion on Malondialdehyde Concentration and the Activity of Antioxidant Enzymes in Rat Erythrocytes

2010 ◽  
Vol 11 (6) ◽  
pp. 2443-2452 ◽  
Author(s):  
José A. Morales-González ◽  
José Gutiérrez-Salinas ◽  
Liliana García-Ortiz ◽  
María Del Carmen Chima-Galán ◽  
Eduardo Madrigal-Santillán ◽  
...  
Keyword(s):  
Antioxidant Enzymes ◽  
Sodium Fluoride ◽  
Rat Erythrocytes

scholarly journals In Vivo Protective Effects of Gallic Acid Isolated from Peltiphyllum Peltatum Against Sodium Fluoride-Induced Oxidative Stress in Rat Erythrocytes

2013 ◽  
Vol 64 (4) ◽  
pp. 553-559 ◽  
Author(s):  
Seyed Fazel Nabavi ◽  
Solomon Habtemariam ◽  
Antoni Sureda ◽  
Akbar Hajizadeh Moghaddam ◽  
Maria Daglia ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Vitamin C ◽  
Gallic Acid ◽  
Sodium Fluoride ◽  
Enzyme Activities ◽  
Control Group ◽  
Rat Erythrocytes ◽  
Pre Treatment

Abstract Gallic acid has been identified as an antioxidant component of the edible and medicinal plant Peltiphyllum peltatum. The present study examined its potential protective role against sodium fluoride (NaF)-induced oxidative stress in rat erythrocytes. Oxidative stress was induced by NaF administration through drinking water (1030.675 mg m-3 for one week). Gallic acid at 10 mg kg-1 and 20 mg kg-1 and vitamin C for positive controls (10 mg kg-1) were administered daily intraperitoneally for one week prior to NaF administration. Thiobarbituric acid reactive substances, antioxidant enzyme activities (superoxide dismutase and catalase), and the level of reduced glutathione were evaluated in rat erythrocytes. Lipid peroxidation in NaF-exposed rats significantly increased (by 88.8 %) when compared to the control group (p<0.05). Pre-treatment with gallic acid suppressed lipid peroxidation in erythrocytes in a dose-dependent manner. Catalase and superoxide dismutase enzyme activities and glutathione levels were reduced by NaF intoxication by 54.4 %, 63.69 %, and 42 % (p<0.001; vs. untreated control group), respectively. Pre-treatment with gallic acid or vitamin C significantly attenuated the deleterious effects. Gallic acid isolated from Peltiphyllum peltatum and vitamin C mitigated the NaF-induced oxidative stress in rat erythrocytes.

scholarly journals The Relationship Between Antioxidant Enzymes and Lipid Peroxidation in Senescent Rat Erythrocytes

2015 ◽  
pp. 891-896 ◽  
Author(s):  
M. MLADENOV ◽  
M. GOKIK ◽  
N. HADZI-PETRUSHEV ◽  
I. GJORGOSKI ◽  
N. JANKULOVSKI
Keyword(s):  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Uric Acid ◽  
Antioxidant Enzymes ◽  
Glutathione Peroxidase ◽  
Complete Information ◽  
Rat Erythrocytes ◽  
Endogenous Antioxidants ◽  
The Relationship

The aim of this study was to gain more complete information about the relationships between some endogenous antioxidants and the malondialdehyde (MDA) as a marker of lipid peroxidation, during D-galactose induced senescence. The activities of superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) and the concentrations of uric acid (UA) in plasma and MDA in erythrocyte’s hemolysate, were determined in 15 D-galactose (D-gal), treated rats and compared with 15 placebo. The activity of the erythrocyte’s CAT was found significantly increased due to the senescence. The ratio of the activities of antioxidant enzymes R=SOD/(GPx+CAT) was significantly decreased due to the senescence and negatively correlated with the MDA (ρ=–0.524, p=0.045). The antioxidant enzymes SOD and GPx negatively correlated with the MDA, while CAT displayed no correlation. Further, the UA positively correlated with the ratio of activities of the antioxidant enzymes R=SOD/(GPx+CAT), (ρ=0.564, p=0.029 for senescent rats). Obtained results may contribute to better understanding of the process of D-gal induced senescence in the erythrocytes.

Effects of methomyl on lipid peroxidation and antioxidant enzymes in rat erythrocytes: In vitro studies

2009 ◽  
Vol 25 (8) ◽  
pp. 557-563 ◽  
Author(s):  
Sameeh A Mansour ◽  
Abdel-Tawab H Mossa ◽  
Tarek M Heikal
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Enzymes ◽  
Oxidative Damage ◽  
Oxidative Stress Response ◽  
Dependent Manner ◽  
Glutathione S Transferase ◽  
Rat Erythrocytes ◽  
Concentration Dependent Manner ◽  
Convenient Model

Erythrocytes are a convenient model to understand the membrane oxidative damage induced by various xenobiotic pro-oxidants. This study was designed to investigate the possibility of methomyl (Lannate® 90% SP), S-methyl N-(methylcarbamoyloxy) thioacetimidate, to induce oxidative stress response in rat erythrocytes in vitro. Erythrocytes were incubated for 4 hours at 37°C with different concentrations (0.0, 0.1, 0.5, 1.0, 1.5 and 2.0 mM) of methomyl. The results showed that methomyl decreased acetylcholinesterase (AChE), superoxide dismutase (SOD) and glutathione S-transferase (GST) activities and increased level of lipid peroxidation (LPO) as well as the percentage of haemolysis. The response occurred in a concentration-dependent manner. The study suggested that methomyl has the capability to induce oxidative damage as evidenced by increasing LPO and perturbations in various antioxidant enzymes.

Cytoprotective Effects of Curcumin on Sodium Fluoride-Induced Intoxication in Rat Erythrocytes

2011 ◽  
Vol 88 (3) ◽  
pp. 486-490 ◽  
Author(s):  
Seyed Fazel Nabavi ◽  
Seyed Mohammad Nabavi ◽  
Fatemeh Abolhasani ◽  
Akbar Hajizadeh Moghaddam ◽  
Shahram Eslami
Keyword(s):  
Sodium Fluoride ◽  
Rat Erythrocytes

scholarly journals In Vitro Effect of Sodium Fluoride on Malondialdehyde Concentration and on Superoxide Dismutase, Catalase, and Glutathione Peroxidase in Human Erythrocytes

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
José Gutiérrez-Salinas ◽  
Liliana García-Ortíz ◽  
José A. Morales González ◽  
Sergio Hernández-Rodríguez ◽  
Sotero Ramírez-García ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Antioxidant Enzymes ◽  
Vitamin E ◽  
Glutathione Peroxidase ◽  
Sodium Fluoride ◽  
Specific Activity ◽  
Human Erythrocytes ◽  
Vitro Effect

The aim of this paper was to describe the in vitro effect of sodium fluoride (NaF) on the specific activity of the major erythrocyte antioxidant enzymes, as well as on the membrane malondialdehyde concentration, as indicators of oxidative stress. For this purpose, human erythrocytes were incubated with NaF (0, 7, 28, 56, and 100 μg/mL) or NaF (100 μg/mL) + vitamin E (1, 2.5, 5 and 10 μg/mL). The malondialdehyde (MDA) concentration on the surface of the erythrocytes was determined, as were the enzymatic activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GlPx). Our results demonstrated that erythrocytes incubated with increasing NaF concentrations had an increased MDA concentration, along with decreased activity of antioxidant enzymes. The presence of vitamin E partially reversed the toxic effects of NaF on erythrocytes. These findings suggest that NaF induces oxidative stress in erythrocytes in vitro, and this stress is partially reversed by the presence of vitamin E.

Protective effects of quercetin against sodium fluoride-induced oxidative stress in rat erythrocytes

2011 ◽  
Vol 93 (8) ◽  
pp. 1666-1675
Author(s):  
Seyed Fazel Nabavi ◽  
Shahram Eslami ◽  
Akbar Hajizadeh Moghaddam ◽  
Naser Jafari ◽  
Seyed Mohammad Nabavi ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Sodium Fluoride ◽  
Protective Effects ◽  
Rat Erythrocytes

scholarly journals Effect of Ocimum sanctum in Sodium fluoride (NaF) induced Fluorosis in Rats: A Study with Respect to Antioxidant Enzymes and Fluorosis Markers

2022 ◽  
Vol 56 (1) ◽  
pp. 175-183
Author(s):  
Kondeti Ramudu Shanmugam ◽  
Bhasha Shanmugam ◽  
Mavulapati Siva ◽  
Sahukari Ravi ◽  
Kesireddy Sathyavelu Reddy
Keyword(s):  
Antioxidant Enzymes ◽  
Sodium Fluoride ◽  
Ocimum Sanctum
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