scholarly journals Structural Modeling and Biochemical Characterization of Recombinant KPN_02809, a Zinc-Dependent Metalloprotease from Klebsiella pneumoniae MGH 78578

2012 ◽  
Vol 13 (1) ◽  
pp. 901-917 ◽  
Author(s):  
Mun Teng Wong ◽  
Sy Bing Choi ◽  
Chee Sian Kuan ◽  
Siang Ling Chua ◽  
Chiat Han Chang ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Biochemical Characterization ◽  
Structural Modeling

Genetic and biochemical characterization of GES-5, an extended-spectrum class A β-lactamase from Klebsiella pneumoniae

2007 ◽  
Vol 58 (4) ◽  
pp. 465-468 ◽  
Author(s):  
Il Kwon Bae ◽  
You-Nae Lee ◽  
Seok Hoon Jeong ◽  
Seong Geun Hong ◽  
Jung Hun Lee ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Biochemical Characterization ◽  
Class A ◽  
Extended Spectrum

scholarly journals Biochemical Characterization of SHV-55, an Extended-Spectrum Class A β-Lactamase from Klebsiella pneumoniae

2008 ◽  
Vol 52 (5) ◽  
pp. 1897-1898 ◽  
Author(s):  
Nuno Mendonça ◽  
Richard Bonnet ◽  
Manuela Caniça ◽  
Vera Manageiro
Keyword(s):  
Klebsiella Pneumoniae ◽  
Biochemical Characterization ◽  
Class A ◽  
Extended Spectrum

scholarly journals Molecular and Biochemical Characterization of SHV-56, a Novel Inhibitor-Resistant β-Lactamase from Klebsiella pneumoniae

2008 ◽  
Vol 52 (10) ◽  
pp. 3792-3794 ◽  
Author(s):  
Véronique Dubois ◽  
Laurent Poirel ◽  
François Demarthe ◽  
Corinne Arpin ◽  
Laure Coulange ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Biochemical Characterization ◽  
Clinical Strain ◽  
Chromosomal Gene ◽  
Inhibitor Resistance ◽  
Critical Site ◽  
Single Substitution

ABSTRACT A clinical strain of Klebsiella pneumoniae was found to possess the chromosomal gene bla SHV-56, encoding a new inhibitor-resistant β-lactamase with a pI of 7.6. SHV-56 is derived from SHV-11 by the single substitution K234R. This mutation therefore evidences a new critical site for inhibitor resistance among SHV enzymes.

scholarly journals Biochemical Characterization of the TEM-107 Extended-Spectrum β-Lactamase in a Klebsiella pneumoniae Isolate from South Korea

2011 ◽  
Vol 55 (12) ◽  
pp. 5930-5932 ◽  
Author(s):  
Kyungwon Lee ◽  
Jong Hwa Yum ◽  
Dongeun Yong ◽  
Seok Hoon Jeong ◽  
Gian Maria Rossolini ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
South Korea ◽  
Clavulanic Acid ◽  
Clinical Isolate ◽  
Biochemical Characterization ◽  
Content Type ◽  
Extended Spectrum

ABSTRACTThe TEM-107 extended-spectrum β-lactamase detected in aKlebsiella pneumoniaeclinical isolate had a Gly238Ser substitution compared to the TEM-43 β-lactamase. The MIC of ceftazidime was higher (64 μg/ml) than that of cefotaxime (2 μg/ml) for the isolate. Clavulanic acid reduced the MIC of ceftazidime 64-fold.

Biochemical Characterization of CTX-M-15 ESβL purified from clinical strain of Klebsiella pneumoniae SJ16

2021 ◽  
pp. 4080-4084
Author(s):  
Layla T. Yassen ◽  
Saad L. Hamed ◽  
Ban O. Abdulsattar ◽  
Asmaa A. Hussein
Keyword(s):  
Enzyme Activity ◽  
Klebsiella Pneumoniae ◽  
Biochemical Characterization ◽  
Specific Activity ◽  
Copper Chloride ◽  
Gel Filtration ◽  
Clinical Strain ◽  
Minor Effect ◽  
A Minor

The aim of this study was purification and characterization of CTX-M-15 as a medically important enzyme from locally Klebsiella pneumoniae isolate, CTX-M-15 enzyme was subjected to two purification steps including: precipitation with 80% ammonium sulfate saturation and gel filtration chromatography by using Sepharose -6B column. Specific activity of purified enzyme has been increment up to 21.9 IU/mg with 7.3 purification folds and 69% enzyme recapture. Characterization study of purified enzyme demonstrated that the M.wt. of CTX-M-15 produced by K. pneumoniae was almost 32.2 kDa. The maximal enzyme activity at (pH 7.0), and enzyme settled at pH 6-7. The enzyme also revealed a full activity at a range of temperature between 30-37oC. Enzyme activity has inhibited powerfully in the existence of EDTA and calcium chloride, when added separately at a constant concentration. Moreover, copper chloride, and ferric chloride also caused a strong inhibition to the enzyme activity while cloxacillin showed a minor effect on enzyme activity.

scholarly journals Genetic and Biochemical Characterization of OXA-519, a Novel OXA-48-Like β-Lactamase

2018 ◽  
Vol 62 (8) ◽  
Author(s):  
Laura Dabos ◽  
Pierre Bogaerts ◽  
Remy A. Bonnin ◽  
Agustin Zavala ◽  
Pierre Sacré ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Biochemical Characterization ◽  
Multidrug Resistant ◽  
Biochemical Tests ◽  
Content Type

ABSTRACT A multidrug-resistant Klebsiella pneumoniae 1210 isolate with reduced carbapenem susceptibility revealed the presence of a novel plasmid-encoded blaOXA-48-like gene, named blaOXA-519. The 60.7-kb plasmid (pOXA-519) was similar to the IncL-OXA-48 prototypical plasmid except for a ca. 2-kb deletion due to an IS1R insertion. OXA-519 differed from OXA-48 by a Val120Leu substitution, which resulted in an overall reduced β-lactam-hydrolysis profile, except those for ertapenem and meropenem, which were increased. Thus, detection of OXA-519 producers using biochemical tests that monitor imipenem hydrolysis will be difficult.

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