scholarly journals Molecular and Biochemical Characterization of SHV-56, a Novel Inhibitor-Resistant β-Lactamase from Klebsiella pneumoniae

2008 ◽  
Vol 52 (10) ◽  
pp. 3792-3794 ◽  
Author(s):  
Véronique Dubois ◽  
Laurent Poirel ◽  
François Demarthe ◽  
Corinne Arpin ◽  
Laure Coulange ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Biochemical Characterization ◽  
Clinical Strain ◽  
Chromosomal Gene ◽  
Inhibitor Resistance ◽  
Critical Site ◽  
Single Substitution

ABSTRACT A clinical strain of Klebsiella pneumoniae was found to possess the chromosomal gene bla SHV-56, encoding a new inhibitor-resistant β-lactamase with a pI of 7.6. SHV-56 is derived from SHV-11 by the single substitution K234R. This mutation therefore evidences a new critical site for inhibitor resistance among SHV enzymes.

Biochemical Characterization of CTX-M-15 ESβL purified from clinical strain of Klebsiella pneumoniae SJ16

2021 ◽  
pp. 4080-4084
Author(s):  
Layla T. Yassen ◽  
Saad L. Hamed ◽  
Ban O. Abdulsattar ◽  
Asmaa A. Hussein
Keyword(s):  
Enzyme Activity ◽  
Klebsiella Pneumoniae ◽  
Biochemical Characterization ◽  
Specific Activity ◽  
Copper Chloride ◽  
Gel Filtration ◽  
Clinical Strain ◽  
Minor Effect ◽  
A Minor

The aim of this study was purification and characterization of CTX-M-15 as a medically important enzyme from locally Klebsiella pneumoniae isolate, CTX-M-15 enzyme was subjected to two purification steps including: precipitation with 80% ammonium sulfate saturation and gel filtration chromatography by using Sepharose -6B column. Specific activity of purified enzyme has been increment up to 21.9 IU/mg with 7.3 purification folds and 69% enzyme recapture. Characterization study of purified enzyme demonstrated that the M.wt. of CTX-M-15 produced by K. pneumoniae was almost 32.2 kDa. The maximal enzyme activity at (pH 7.0), and enzyme settled at pH 6-7. The enzyme also revealed a full activity at a range of temperature between 30-37oC. Enzyme activity has inhibited powerfully in the existence of EDTA and calcium chloride, when added separately at a constant concentration. Moreover, copper chloride, and ferric chloride also caused a strong inhibition to the enzyme activity while cloxacillin showed a minor effect on enzyme activity.

Genetic and biochemical characterization of GES-5, an extended-spectrum class A β-lactamase from Klebsiella pneumoniae

2007 ◽  
Vol 58 (4) ◽  
pp. 465-468 ◽  
Author(s):  
Il Kwon Bae ◽  
You-Nae Lee ◽  
Seok Hoon Jeong ◽  
Seong Geun Hong ◽  
Jung Hun Lee ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Biochemical Characterization ◽  
Class A ◽  
Extended Spectrum

scholarly journals Biochemical Characterization of SHV-55, an Extended-Spectrum Class A β-Lactamase from Klebsiella pneumoniae

2008 ◽  
Vol 52 (5) ◽  
pp. 1897-1898 ◽  
Author(s):  
Nuno Mendonça ◽  
Richard Bonnet ◽  
Manuela Caniça ◽  
Vera Manageiro
Keyword(s):  
Klebsiella Pneumoniae ◽  
Biochemical Characterization ◽  
Class A ◽  
Extended Spectrum

scholarly journals Structural Modeling and Biochemical Characterization of Recombinant KPN_02809, a Zinc-Dependent Metalloprotease from Klebsiella pneumoniae MGH 78578

2012 ◽  
Vol 13 (1) ◽  
pp. 901-917 ◽  
Author(s):  
Mun Teng Wong ◽  
Sy Bing Choi ◽  
Chee Sian Kuan ◽  
Siang Ling Chua ◽  
Chiat Han Chang ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Biochemical Characterization ◽  
Structural Modeling

scholarly journals Biochemical Characterization of the TEM-107 Extended-Spectrum β-Lactamase in a Klebsiella pneumoniae Isolate from South Korea

2011 ◽  
Vol 55 (12) ◽  
pp. 5930-5932 ◽  
Author(s):  
Kyungwon Lee ◽  
Jong Hwa Yum ◽  
Dongeun Yong ◽  
Seok Hoon Jeong ◽  
Gian Maria Rossolini ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
South Korea ◽  
Clavulanic Acid ◽  
Clinical Isolate ◽  
Biochemical Characterization ◽  
Content Type ◽  
Extended Spectrum

ABSTRACTThe TEM-107 extended-spectrum β-lactamase detected in aKlebsiella pneumoniaeclinical isolate had a Gly238Ser substitution compared to the TEM-43 β-lactamase. The MIC of ceftazidime was higher (64 μg/ml) than that of cefotaxime (2 μg/ml) for the isolate. Clavulanic acid reduced the MIC of ceftazidime 64-fold.

scholarly journals Molecular and Biochemical Characterization of CTX-M-131, a Natural Asp240Gly Variant Derived from CTX-M-2, Produced by a Providencia rettgeri Clinical Strain in São Paulo, Brazil

2015 ◽  
Vol 59 (3) ◽  
pp. 1815-1817 ◽  
Author(s):  
Milena Dropa ◽  
Barbara Ghiglione ◽  
Maria Helena Matté ◽  
Livia Carminato Balsalobre ◽  
Nilton Lincopan ◽  
...  
Keyword(s):  
Biochemical Characterization ◽  
Clinical Strain ◽  
Class 1 Integron ◽  
Providencia Rettgeri ◽  
Content Type ◽  
Steady State Kinetic ◽  
Class 1 ◽  
Encoding Genes ◽  
State Kinetic

ABSTRACTCTX-M-131 is a natural Asp240Gly variant from the CTX-M-2 group detected in aProvidencia rettgericlinical strain from Brazil. Molecular analysis showed thatblaCTX-M-131was inserted in a complex class 1 integron harbored by a 112-kb plasmid, which has not been previously described as a platform for CTX-M-encoding genes with the Asp240Gly mutation. Steady-state kinetic parameters showed that the enzyme has a typical cefotaximase catalytic profile and an enhanced activity against ceftazidime.

scholarly journals Molecular and Biochemical Characterization of OXA-45, an Extended-Spectrum Class 2d′ β-Lactamase in Pseudomonas aeruginosa

2003 ◽  
Vol 47 (9) ◽  
pp. 2859-2863 ◽  
Author(s):  
Mark A. Toleman ◽  
Kenneth Rolston ◽  
Ronald N. Jones ◽  
Timothy R. Walsh
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Biochemical Characterization ◽  
Polymyxin B ◽  
Clinical Strain ◽  
Surveillance Program ◽  
Extended Spectrum ◽  
Class D ◽  
Antimicrobial Surveillance ◽  
Serovar Typhimurium

ABSTRACT As part of the CANCER Antimicrobial Surveillance Program in North America, a clinical strain of Pseudomonas aeruginosa, strain 07-406, isolated in Texas was found to be resistant to all antimicrobials except polymyxin B. Genetic analysis of this isolate identified two unique extended-spectrum β-lactamase genes. One, bla VIM-7, encoded a metallo-β-lactamase (unpublished data), and the other, bla OXA-45, described here, encoded a class D extended-spectrum β-lactamase. bla OXA-45 was isolated on a Sau3A1 genomic fragment of 1.8 kb and encodes a protein of 264 amino acids with the highest identities to OXA-18 (65.9%), OXA-9 (42.8%), OXA-22 (40.2%), OXA-12 (38.6%), and OXA-29 (35.2%) but weak identities with other class D β-lactamases. bla OXA-45 was found to be harbored on a 24-kb plasmid in a region that displays high identities with a section of the 43-kb genomic island of Salmonella enterica serovar Typhimurium DT104. Biochemically OXA-45 is most similar to OXA-18 in its substrate profile and inhibition by clavulanic acid and is a member of the 2d′ class of β-lactamases.

scholarly journals Genetic and Biochemical Characterization of OXA-519, a Novel OXA-48-Like β-Lactamase

2018 ◽  
Vol 62 (8) ◽  
Author(s):  
Laura Dabos ◽  
Pierre Bogaerts ◽  
Remy A. Bonnin ◽  
Agustin Zavala ◽  
Pierre Sacré ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Biochemical Characterization ◽  
Multidrug Resistant ◽  
Biochemical Tests ◽  
Content Type

ABSTRACT A multidrug-resistant Klebsiella pneumoniae 1210 isolate with reduced carbapenem susceptibility revealed the presence of a novel plasmid-encoded blaOXA-48-like gene, named blaOXA-519. The 60.7-kb plasmid (pOXA-519) was similar to the IncL-OXA-48 prototypical plasmid except for a ca. 2-kb deletion due to an IS1R insertion. OXA-519 differed from OXA-48 by a Val120Leu substitution, which resulted in an overall reduced β-lactam-hydrolysis profile, except those for ertapenem and meropenem, which were increased. Thus, detection of OXA-519 producers using biochemical tests that monitor imipenem hydrolysis will be difficult.

Sign in / Sign up

Export Citation Format

Share Document