scholarly journals Gene sequence and biochemical characterization of FOX-1 from Klebsiella pneumoniae, a new AmpC-type plasmid-mediated beta-lactamase with two molecular variants.

1994 ◽  
Vol 38 (9) ◽  
pp. 2150-2157 ◽  
Author(s):  
M Gonzalez Leiza ◽  
J C Perez-Diaz ◽  
J Ayala ◽  
J M Casellas ◽  
J Martinez-Beltran ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Gene Sequence ◽  
Biochemical Characterization ◽  
Beta Lactamase ◽  
Molecular Variants

Transferable cefoxitin resistance in enterobacteria from Greek hospitals and characterization of a plasmid-mediated group 1 beta-lactamase (LAT-2).

1996 ◽  
Vol 40 (7) ◽  
pp. 1736-1740 ◽  
Author(s):  
M Gazouli ◽  
L S Tzouvelekis ◽  
E Prinarakis ◽  
V Miriagou ◽  
E Tzelepi
Keyword(s):  
Escherichia Coli ◽  
Amino Acids ◽  
Klebsiella Pneumoniae ◽  
Enterobacter Aerogenes ◽  
Citrobacter Freundii ◽  
Beta Lactamase ◽  
Beta Lactamases ◽  
Greek Hospitals ◽  
Group 1

Cefoxitin resistance in Klebsiella pneumoniae from Escherichia coli strains isolated in Greek hospitals was found to be due to the acquisition of similar plasmids coding for group 1 beta-lactamases. The plasmids were not self-transferable but were mobilized by conjugative plasmids. These elements have also been spread to Enterobacter aerogenes. The most common enzyme was a Citrobacter freundii-derived cephalosporinase (LAT-2) which differed from LAT-1 by three amino acids.

Genetic and biochemical characterization of GES-5, an extended-spectrum class A β-lactamase from Klebsiella pneumoniae

2007 ◽  
Vol 58 (4) ◽  
pp. 465-468 ◽  
Author(s):  
Il Kwon Bae ◽  
You-Nae Lee ◽  
Seok Hoon Jeong ◽  
Seong Geun Hong ◽  
Jung Hun Lee ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Biochemical Characterization ◽  
Class A ◽  
Extended Spectrum

scholarly journals Biochemical Characterization of SHV-55, an Extended-Spectrum Class A β-Lactamase from Klebsiella pneumoniae

2008 ◽  
Vol 52 (5) ◽  
pp. 1897-1898 ◽  
Author(s):  
Nuno Mendonça ◽  
Richard Bonnet ◽  
Manuela Caniça ◽  
Vera Manageiro
Keyword(s):  
Klebsiella Pneumoniae ◽  
Biochemical Characterization ◽  
Class A ◽  
Extended Spectrum

CHARACTERIZATION OF KLEBSIELLA PNEUMONIAE AND ESCHERICHIA COLI STRAINS THAT PRODUCE CTX-M-2-TYPE BROAD SPECTRUM BETA-LACTAMASE ISOLATED FROM A CHILD WITH LEUKEMIA

2002 ◽  
Vol 21 (3) ◽  
pp. 260-262 ◽  
Author(s):  
Toshiya Ohkawa ◽  
Masao Yoshinaga ◽  
Naoaki Ikarimoto ◽  
Hiroaki Miyanohara ◽  
Koichiro Miyata ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Klebsiella Pneumoniae ◽  
Broad Spectrum ◽  
Beta Lactamase

scholarly journals Structural Modeling and Biochemical Characterization of Recombinant KPN_02809, a Zinc-Dependent Metalloprotease from Klebsiella pneumoniae MGH 78578

2012 ◽  
Vol 13 (1) ◽  
pp. 901-917 ◽  
Author(s):  
Mun Teng Wong ◽  
Sy Bing Choi ◽  
Chee Sian Kuan ◽  
Siang Ling Chua ◽  
Chiat Han Chang ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Biochemical Characterization ◽  
Structural Modeling

scholarly journals Molecular and Biochemical Characterization of SHV-56, a Novel Inhibitor-Resistant β-Lactamase from Klebsiella pneumoniae

2008 ◽  
Vol 52 (10) ◽  
pp. 3792-3794 ◽  
Author(s):  
Véronique Dubois ◽  
Laurent Poirel ◽  
François Demarthe ◽  
Corinne Arpin ◽  
Laure Coulange ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Biochemical Characterization ◽  
Clinical Strain ◽  
Chromosomal Gene ◽  
Inhibitor Resistance ◽  
Critical Site ◽  
Single Substitution

ABSTRACT A clinical strain of Klebsiella pneumoniae was found to possess the chromosomal gene bla SHV-56, encoding a new inhibitor-resistant β-lactamase with a pI of 7.6. SHV-56 is derived from SHV-11 by the single substitution K234R. This mutation therefore evidences a new critical site for inhibitor resistance among SHV enzymes.

scholarly journals Biochemical Characterization of the TEM-107 Extended-Spectrum β-Lactamase in a Klebsiella pneumoniae Isolate from South Korea

2011 ◽  
Vol 55 (12) ◽  
pp. 5930-5932 ◽  
Author(s):  
Kyungwon Lee ◽  
Jong Hwa Yum ◽  
Dongeun Yong ◽  
Seok Hoon Jeong ◽  
Gian Maria Rossolini ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
South Korea ◽  
Clavulanic Acid ◽  
Clinical Isolate ◽  
Biochemical Characterization ◽  
Content Type ◽  
Extended Spectrum

ABSTRACTThe TEM-107 extended-spectrum β-lactamase detected in aKlebsiella pneumoniaeclinical isolate had a Gly238Ser substitution compared to the TEM-43 β-lactamase. The MIC of ceftazidime was higher (64 μg/ml) than that of cefotaxime (2 μg/ml) for the isolate. Clavulanic acid reduced the MIC of ceftazidime 64-fold.

Biochemical Characterization of CTX-M-15 ESβL purified from clinical strain of Klebsiella pneumoniae SJ16

2021 ◽  
pp. 4080-4084
Author(s):  
Layla T. Yassen ◽  
Saad L. Hamed ◽  
Ban O. Abdulsattar ◽  
Asmaa A. Hussein
Keyword(s):  
Enzyme Activity ◽  
Klebsiella Pneumoniae ◽  
Biochemical Characterization ◽  
Specific Activity ◽  
Copper Chloride ◽  
Gel Filtration ◽  
Clinical Strain ◽  
Minor Effect ◽  
A Minor

The aim of this study was purification and characterization of CTX-M-15 as a medically important enzyme from locally Klebsiella pneumoniae isolate, CTX-M-15 enzyme was subjected to two purification steps including: precipitation with 80% ammonium sulfate saturation and gel filtration chromatography by using Sepharose -6B column. Specific activity of purified enzyme has been increment up to 21.9 IU/mg with 7.3 purification folds and 69% enzyme recapture. Characterization study of purified enzyme demonstrated that the M.wt. of CTX-M-15 produced by K. pneumoniae was almost 32.2 kDa. The maximal enzyme activity at (pH 7.0), and enzyme settled at pH 6-7. The enzyme also revealed a full activity at a range of temperature between 30-37oC. Enzyme activity has inhibited powerfully in the existence of EDTA and calcium chloride, when added separately at a constant concentration. Moreover, copper chloride, and ferric chloride also caused a strong inhibition to the enzyme activity while cloxacillin showed a minor effect on enzyme activity.

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