scholarly journals In Vitro Anti-Inflammatory and Cytotoxic Effects of Aqueous Extracts from the Edible Sea Anemones Anemonia sulcata and Actinia equina

2017 ◽  
Vol 18 (3) ◽  
pp. 653 ◽  
Author(s):  
Tânia Silva ◽  
Paula de Andrade ◽  
Fátima Paiva-Martins ◽  
Patrícia Valentão ◽  
David Pereira
Keyword(s):  
Aqueous Extracts ◽  
Sea Anemones ◽  
Cytotoxic Effects ◽  
Anemonia Sulcata ◽  
Anti Inflammatory

scholarly journals Phenolic Compounds in Organic and Aqueous Extracts from Acacia farnesiana Pods Analyzed by ULPS-ESI-Q-oa/TOF-MS. In Vitro Antioxidant Activity and Anti-Inflammatory Response in CD-1 Mice

Molecules ◽  
2018 ◽  
Vol 23 (9) ◽  
pp. 2386 ◽  
Author(s):  
Delgadillo Claudia ◽  
Cuchillo-Hilario Mario ◽  
Navarro Arturo ◽  
Medina-Campos Omar Noel ◽  
Nieto Antonio ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Quinic Acid ◽  
Aqueous Extracts ◽  
Acacia Farnesiana ◽  
Tnf Α ◽  
Anti Inflammatory ◽  
Tbars Assay ◽  
Galloyl Glucose ◽  
Tof Ms

Background: Acacia farnesiana (AF) pods have been traditionally used to treat dyspepsia, diarrhea and topically for dermal inflammation. Main objectives: (1) investigate the antioxidant activity and protection against oxidative-induced damage of six extracts from AF pods and (2) their capacity to curb the inflammation process as well as to down-regulate the pro-inflammatory mediators. Methods: Five organic extracts (chloroformic, hexanic, ketonic, methanolic, methanolic:aqueous and one aqueous extract) were obtained and analyzed by UPLC-ESI-Q-oa/TOF-MS. Antioxidant activity (DPPH•, ORAC and FRAP assays) and lipid peroxidation (TBARS assay) were performed. Assessment of anti-inflammatory properties was made by the ear edema induced model in CD-1 mice and MPO activity assay. Likewise, histological analysis, IL-1β, IL-6, IL-10, TNF-α, COX measurements plus nitrite and immunohistochemistry analysis were carried out. Results: Methyl gallate, gallic acid, galloyl glucose isomer 1, galloyl glucose isomer 2, galloyl glucose isomer 3, digalloyl glucose isomer 1, digalloyl glucose isomer 2, digalloyl glucose isomer 3, digalloyl glucose isomer 4, hydroxytyrosol acetate, quinic acid, and caffeoylmalic acid were identified. Both organic and aqueous extracts displayed antioxidant activity. All extracts exhibited a positive effect on the interleukins, COX and immunohistochemistry assays. Conclusion: All AF pod extracts can be effective as antioxidant and topical anti-inflammatory agents.

In vitro study of iron coordination properties, anti-inflammatory potential, and cytotoxic effects of N-salicylidene and N-vanillidene anil Schiff bases

2018 ◽  
Vol 72 (9) ◽  
pp. 2171-2180 ◽  
Author(s):  
Zorica D. Petrović ◽  
Jelena Đorović ◽  
Dušica Simijonović ◽  
Snežana Trifunović ◽  
Vladimir P. Petrović
Keyword(s):  
Schiff Bases ◽  
In Vitro Study ◽  
Vitro Study ◽  
Cytotoxic Effects ◽  
Coordination Properties ◽  
Iron Coordination ◽  
Anti Inflammatory

scholarly journals Inhibition of Urease, Elastase, and β-Glucuronidase Enzymatic Activity by Applying Aqueous Extracts of Opuntia oligacantha C.F. Först Acid Fruits: In Vitro Essay under Simulated Digestive Conditions

2021 ◽  
Vol 11 (16) ◽  
pp. 7705
Author(s):  
Gabriela Medina-Pérez ◽  
Laura Peralta-Adauto ◽  
Laura Afanador-Barajas ◽  
Fabián Fernández-Luqueño ◽  
Elizabeth Pérez-Soto ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
Inflammatory Diseases ◽  
Strong Acid ◽  
Aqueous Extracts ◽  
Inhibition Activity ◽  
Gastrointestinal Conditions ◽  
Excessive Intake ◽  
Anti Inflammatory ◽  
High Doses

Non-communicable diseases such as gastric inflammatory diseases and the hepatic pathologies are mainly related to bad lifestyle habits such as recurrent consumption of non-steroidal anti-inflammatory drugs (NSAIDs), excessive intake of alcohol, tobacco, steroids (high doses), alkaline agents, strong acid foods, and high-fat food, and Helicobacter pylori infections, among others. The fruit of Opuntia oligacantha C.F. Först var. Ulapa (xoconostle) is currently being studied due its nutritional and functional properties. The objective of the present study was to evaluate gastroprotective, anti-inflammatory, and hepatoprotective activities of different parts of xoconostle fruit by establishing in vitro simulated gastrointestinal conditions. Four treatments were established to test aqueous extracts (pericarp (P), mesocarp (M), endocarp (E) and whole fruit (W)). The quantified bioactive compounds were the total phenols, flavonoids, tannins, and betalains. The enzymatic assays were: urease, elastase, and β-glucuronidase. Significant differences (p < 0.05) of bioactive compounds content were measured in xoconostle extracts, the highest concentration was found in W (phenols 313 mg GAE/100 g, flavonoids 189 mg QE/100 g, tannins 71 mg CATE/100 g). The betalains content was higher in E; 17 mg/100 g significant differences were observed (p < 0.05) in the enzymatic inhibitions test (urease, elastase and β-glucuronidase), where W presented the highest inhibition activity (86%, 79%, and 84%), respectively. Bioactive compounds after in vitro gastrointestinal tests were maintained above 60% enzymatic inhibition activity.

scholarly journals In-Vivo and In-Vitro Anti-Inflammatory Activities of the Aqueous Extract of Di-Herbal Formulation (Euphorbia hirta and lactuca virosa)

2021 ◽  
Vol 24 (11) ◽  
pp. 1979-1985
Author(s):  
DO Uwaya ◽  
R Okakwu ◽  
OP Omozuwa
Keyword(s):  
Protein Denaturation ◽  
Aqueous Extracts ◽  
Membrane Stabilization ◽  
Euphorbia Hirta ◽  
Lactuca Virosa ◽  
Herbal Formulation ◽  
Inflammatory Protein ◽  
Anti Inflammatory

Euphorbia hirta and Lactuca virosa are medicinal plants that have been used in the cure and treatment of various diseases and for health care. This study aims at evaluating the anti-inflammatory activities of the aqueous extracts of Euphorbia hirta and Lactuca virosa plants. Albumin and histamine induced inflammation in mice and xylene induced ear edema were used for the in-vivo anti-inflammatory studies. Erythrocyte membrane stabilization and inhibition of protein denaturation assays were used for the in-vitro anti-inflammatory studies. Combined doses of 100 mg/kg Euphorbia hirta and 50 mg/kg Lactuca virosa, 100 mg/kg Euphorbia hirta and 100 mg/kg Lactuca virosa, 100 mg/kg Lactuca virosa only and 10 mg/kg diclofenac significantly reduced inflamed paw in mice (P<0.05) compared to control in albumin and histamine induced inflammatory test. Combined doses of 50 mg/kg Euphorbia hirta and 100 mg/kg Lactuca virosa,100 mg/kg Euphorbia hirta, 100mg/kg Lactuca virosa significantly reduced xylene induced inflammation (P<0.001) compared to control. The extracts at 1 mg/ml, 2 mg/ml and 3 mg/ml significantly inhibited protein denaturation (P<0.001) and heat induced hemolysis of erythrocytes (P<0.0001). The plant extract of Euphorbia hirta and lactuca virosa possesses in-vivo and in-vivo anti-inflammatory effects. Keywords: Euphorbia hirta, Lactuca virosa, anti-inflammatory, protein denaturation

Sesquiterpene Lactones from Dimerostemma Species (Asteraceae) and in vitro Potential Anti-Inflammatory Activities

2006 ◽  
Vol 61 (9-10) ◽  
pp. 647-652 ◽  
Author(s):  
Ricardo Stefani ◽  
Karin Schorr ◽  
Juliana Maria Tureta ◽  
Walter Vichnewski ◽  
Irmgard Merfort ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Dna Binding ◽  
Sesquiterpene Lactones ◽  
Raw 264.7 Cells ◽  
Structure Identification ◽  
Human Neutrophils ◽  
Raw 264.7 ◽  
Cytotoxic Effects ◽  
Anti Inflammatory

AbstractTwo Brazilian species of Dimerostemma (Asteraceae) were chemically investigated. Two known sesquiterpene lactones (STLs), a germacrolide and an eudesmanolide, were isolated from D. episcopale while D. brasilianum afforded the new germacranolide 1β,5β,10α-trihydroxy- 8α-angeloyloxy-germacra-3,11(13)-dien-6α,12-olide in addition to a known one. Structure identification based on NMR and MS analyses. 1β,10α,4α,5β-Diepoxy-8α-angeloyloxycostunolide isolated from D. brasilianum was studied for its anti-inflammatory activity. This STL completely inhibited DNA binding of the transcription factor NF-κB at a concentration of 5 μм and 10 μм in Jurkat T and Raw 264.7 cells, respectively. Elastase release from human neutrophils was reduced to 50% at a concentrations of 23 μм after stimulation with PAF and of 27 μм after stimulation with fMLP without showing cytotoxic effects. Additionally, elastase was also directly inhibited.

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