scholarly journals Proton Therapy and Src Family Kinase Inhibitor Combined Treatments on U87 Human Glioblastoma Multiforme Cell Line

2019 ◽  
Vol 20 (19) ◽  
pp. 4745 ◽  
Author(s):  
Francesco P Cammarata ◽  
Filippo Torrisi ◽  
Giusi I Forte ◽  
Luigi Minafra ◽  
Valentina Bravatà ◽  
...  
Keyword(s):  
Glioblastoma Multiforme ◽  
Cell Line ◽  
Proton Therapy ◽  
Kinase Inhibitor ◽  
Malignant Gliomas ◽  
Clonogenic Survival ◽  
Quadratic Model ◽  
Linear Quadratic ◽  
Linear Quadratic Model ◽  
New Genes

Glioblastoma Multiforme (GBM) is the most common of malignant gliomas in adults with an exiguous life expectancy. Standard treatments are not curative and the resistance to both chemotherapy and conventional radiotherapy (RT) plans is the main cause of GBM care failures. Proton therapy (PT) shows a ballistic precision and a higher dose conformity than conventional RT. In this study we investigated the radiosensitive effects of a new targeted compound, SRC inhibitor, named Si306, in combination with PT on the U87 glioblastoma cell line. Clonogenic survival assay, dose modifying factor calculation and linear-quadratic model were performed to evaluate radiosensitizing effects mediated by combination of the Si306 with PT. Gene expression profiling by microarray was also conducted after PT treatments alone or combined, to identify gene signatures as biomarkers of response to treatments. Our results indicate that the Si306 compound exhibits a radiosensitizing action on the U87 cells causing a synergic cytotoxic effect with PT. In addition, microarray data confirm the SRC role as the main Si306 target and highlights new genes modulated by the combined action of Si306 and PT. We suggest, the Si306 as a new candidate to treat GBM in combination with PT, overcoming resistance to conventional treatments.

Predicting the clonogenic survival of A549 cells after modulated x-ray irradiation using the linear quadratic model

2008 ◽  
Vol 54 (2) ◽  
pp. 187-206 ◽  
Author(s):  
Regina Bromley ◽  
Lyn Oliver ◽  
Ross Davey ◽  
Rozelle Harvie ◽  
Clive Baldock
Keyword(s):  
A549 Cells ◽  
Clonogenic Survival ◽  
Quadratic Model ◽  
Linear Quadratic ◽  
X Ray ◽  
Linear Quadratic Model

scholarly journals Simulating Radiotherapy Effect in High-Grade Glioma by Using Diffusive Modeling and Brain Atlases

2012 ◽  
Vol 2012 ◽  
pp. 1-9 ◽  
Author(s):  
Alexandros Roniotis ◽  
Kostas Marias ◽  
Vangelis Sakkalis ◽  
Georgios C. Manikis ◽  
Michalis Zervakis
Keyword(s):  
High Grade Glioma ◽  
Quadratic Model ◽  
Normal Brain ◽  
Linear Quadratic ◽  
Linear Quadratic Model ◽  
Diffusive Model ◽  
Glioma Model ◽  
Modern Application ◽  
Diffusion Tensors ◽  
Brain Atlases

Applying diffusive models for simulating the spatiotemporal change of concentration of tumour cells is a modern application of predictive oncology. Diffusive models are used for modelling glioblastoma, the most aggressive type of glioma. This paper presents the results of applying a linear quadratic model for simulating the effects of radiotherapy on an advanced diffusive glioma model. This diffusive model takes into consideration the heterogeneous velocity of glioma in gray and white matter and the anisotropic migration of tumor cells, which is facilitated along white fibers. This work uses normal brain atlases for extracting the proportions of white and gray matter and the diffusion tensors used for anisotropy. The paper also presents the results of applying this glioma model on real clinical datasets.

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