scholarly journals Beta3-Tubulin Is Critical for Microtubule Dynamics, Cell Cycle Regulation, and Spontaneous Release of Microvesicles in Human Malignant Melanoma Cells (A375)

2020 ◽  
Vol 21 (5) ◽  
pp. 1656
Author(s):  
Mohammed O. Altonsy ◽  
Anutosh Ganguly ◽  
Matthias Amrein ◽  
Philip Surmanowicz ◽  
Shu Shun Li ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Malignant Melanoma ◽  
Structural Changes ◽  
Melanoma Cells ◽  
Neoplastic Cell ◽  
Primary Component ◽  
Cellular Growth ◽  
Class Iii ◽  
M Cell ◽  
Human Malignant Melanoma

Microtubules (MTs), microfilaments, and intermediate filaments, the main constituents of the cytoskeleton, undergo continuous structural changes (metamorphosis), which are central to cellular growth, division, and release of microvesicles (MVs). Altered MTs dynamics, uncontrolled proliferation, and increased production of MVs are hallmarks of carcinogenesis. Class III beta-tubulin (β3-tubulin), one of seven β-tubulin isotypes, is a primary component of MT, which correlates with enhanced neoplastic cell survival, metastasis and resistance to chemotherapy. We studied the effects of β3-tubulin gene silencing on MTs dynamics, cell cycle, and MVs release in human malignant melanoma cells (A375). The knockdown of β3-tubulin induced G2/M cell cycle arrest, impaired MTs dynamics, and reduced spontaneous MVs release. Additional studies are therefore required to elucidate the pathophysiologic and therapeutic role of β3-tubulin in melanoma.

scholarly journals Anti-oncogenic MicroRNA-203 Induces Senescence by Targeting E2F3 Protein in Human Melanoma Cells

2012 ◽  
Vol 287 (15) ◽  
pp. 11769-11777 ◽  
Author(s):  
Shunsuke Noguchi ◽  
Takashi Mori ◽  
Yusami Otsuka ◽  
Nami Yamada ◽  
Yuki Yasui ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Growth ◽  
Malignant Melanoma ◽  
Cell Cycle Arrest ◽  
Ectopic Expression ◽  
Melanoma Cells ◽  
Microrna Target ◽  
Human Malignant Melanoma ◽  
Cycle Arrest ◽  
Tissue Specimens

MicroRNAs regulate gene expression by repressing translation or directing sequence-specific degradation of their complementary mRNA. We recently reported that miR-203 is down-regulated, and its exogenous expression inhibits cell growth in canine oral malignant melanoma tissue specimens as well as in canine and human malignant melanoma cells. A microRNA target database predicted E2F3 and ZBP-89 as putative targets of microRNA-203 (miR-203). The expression levels of E2F3a, E2F3b, and ZBP-89 were markedly up-regulated in human malignant melanoma Mewo cells compared with those in human epidermal melanocytes. miR-203 significantly suppressed the luciferase activity of reporter plasmids containing the 3′-UTR sequence of either E2F3 or ZBP-89 complementary to miR-203. The ectopic expression of miR-203 in melanoma cells reduced the levels of E2F3a, E2F3b, and ZBP-89 protein expression. At the same time, miR-203 induced cell cycle arrest and senescence phenotypes, such as elevated expression of hypophosphorylated retinoblastoma and other markers for senescence. Silencing of E2F3, but not of ZBP-89, inhibited cell growth and induced cell cycle arrest and senescence. These results demonstrate a novel role for miR-203 as a tumor suppressor acting by inducing senescence in melanoma cells.

scholarly journals Toxicogenomics of A375 human malignant melanoma cells treated with arbutin

2006 ◽  
Vol 14 (1) ◽  
pp. 87-105 ◽  
Author(s):  
Sun-Long Cheng ◽  
Rosa Huang Liu ◽  
Jin-Nan Sheu ◽  
Shui-Tein Chen ◽  
Supachok Sinchaikul ◽  
...  
Keyword(s):  
Malignant Melanoma ◽  
Melanoma Cells ◽  
Human Malignant Melanoma
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