scholarly journals The LuxI/LuxR-Type Quorum Sensing System Regulates Degradation of Polycyclic Aromatic Hydrocarbons via Two Mechanisms

2020 ◽  
Vol 21 (15) ◽  
pp. 5548
Author(s):  
Zhiliang Yu ◽  
Zeyu Hu ◽  
Qimiao Xu ◽  
Mengting Zhang ◽  
Nate Yuan ◽  
...  
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Quorum Sensing ◽  
Cell Surface ◽  
Aromatic Hydrocarbons ◽  
Bacterial Cell ◽  
Regulatory Gene ◽  
Cell Surface Hydrophobicity ◽  
Surface Hydrophobicity ◽  
Bacterial Cell Surface ◽  
Polycyclic Aromatic

Members of the Sphingomonadales are renowned for their ability to degrade polycyclic aromatic hydrocarbons (PAHs). However, little is known about the regulatory mechanisms of the degradative pathway. Using cross-feeding bioassay, a functional LuxI/LuxR-type acyl-homoserine lactone (AHL)-mediated quorum sensing (QS) system was identified from Croceicoccus naphthovorans PQ-2, a member of the order Sphingomonadales. Inactivation of the QS system resulted in a significant decrease in PAHs degradation. The QS system positively controlled the expression of three PAH-degrading genes (ahdA1e, xylE and xylG) and a regulatory gene ardR, which are located on the large plasmid. Interestingly, the transcription levels of these three PAH-degrading genes were significantly down-regulated in the ardR mutant. In addition, bacterial cell surface hydrophobicity and cell morphology were altered in the QS-deficient mutant. Therefore, the QS system in strain PQ-2 positively regulates PAH degradation via two mechanisms: (i) by induction of PAH-degrading genes directly and/or indirectly; and (ii) by an increase of bacterial cell surface hydrophobicity. The findings of this study improve our understanding of how the QS system influences the degradation of PAHs, therefore facilitating the development of new strategies for the bioremediation of PAHs.

scholarly journals Improvement of the salt aggregation test to study bacterial cell-surface hydrophobicity

1985 ◽  
Vol 30 (1-2) ◽  
pp. 131-138 ◽  
Author(s):  
Ferenc Rozgonyi ◽  
Katalin R. Szitha ◽  
Ã…sa Ljungh ◽  
Suraj B. Baloda ◽  
Stellan Hjertén ◽  
...  
Keyword(s):  
Cell Surface ◽  
Bacterial Cell ◽  
Cell Surface Hydrophobicity ◽  
Surface Hydrophobicity ◽  
Bacterial Cell Surface

Magnolia bark extract increases oral bacterial cell surface hydrophobicity and improves self-perceived breath freshness when added to chewing gum

2016 ◽  
Vol 25 ◽  
pp. 367-374 ◽  
Author(s):  
Stefan W. Wessel ◽  
Henny C. van der Mei ◽  
Anje M. Slomp ◽  
Betsy van de Belt-Gritter ◽  
Amarnath Maitra ◽  
...  
Keyword(s):  
Cell Surface ◽  
Bacterial Cell ◽  
Cell Surface Hydrophobicity ◽  
Surface Hydrophobicity ◽  
Bark Extract ◽  
Chewing Gum ◽  
Bacterial Cell Surface ◽  
Magnolia Bark

Method-induced variation in the bacterial cell surface hydrophobicity MATH test

2021 ◽  
pp. 106234
Author(s):  
Jesús A. Salas-Tovar ◽  
Sarai Escobedo-García ◽  
Guadalupe I. Olivas ◽  
Carlos H. Acosta-Muñiz ◽  
Federico Harte ◽  
...  
Keyword(s):  
Cell Surface ◽  
Bacterial Cell ◽  
Cell Surface Hydrophobicity ◽  
Surface Hydrophobicity ◽  
Bacterial Cell Surface ◽  
Induced Variation ◽  
Math Test

Biofilm formation and changes in bacterial cell surface hydrophobicity during growth in a CAPD model system

2004 ◽  
Vol 56 (7) ◽  
pp. 847-854 ◽  
Author(s):  
G. W. Hanlon ◽  
S. P. Denyer ◽  
N. A. Hodges ◽  
J. A. Brant ◽  
A. B. Lansley ◽  
...  
Keyword(s):  
Cell Surface ◽  
Bacterial Cell ◽  
Biofilm Formation ◽  
Cell Surface Hydrophobicity ◽  
Surface Hydrophobicity ◽  
Model System ◽  
Bacterial Cell Surface

Bacterial Cell-Surface Hydrophobicity

1990 ◽  
pp. 233-244 ◽  
Author(s):  
Ferenc Rozgonyi ◽  
Åsa Ljungh ◽  
Wubshet Mamo ◽  
Stellan Hjertén ◽  
Torkel Wadström
Keyword(s):  
Cell Surface ◽  
Bacterial Cell ◽  
Cell Surface Hydrophobicity ◽  
Surface Hydrophobicity ◽  
Bacterial Cell Surface

Characterization of Microbacterium sp. F10a and its role in polycyclic aromatic hydrocarbon removal in low-temperature soil

2009 ◽  
Vol 55 (5) ◽  
pp. 529-535 ◽  
Author(s):  
X. F. Sheng ◽  
L. Y. He ◽  
L. Zhou ◽  
Y. Y. Shen
Keyword(s):  
Low Temperature ◽  
Polycyclic Aromatic Hydrocarbon ◽  
Cell Surface ◽  
Aromatic Hydrocarbon ◽  
Cell Surface Hydrophobicity ◽  
Surface Hydrophobicity ◽  
Bacterial Inoculation ◽  
Polycyclic Aromatic ◽  
Plant Growth Promoting ◽  
Growth Promoting

A polycyclic aromatic hydrocarbon degrading bacterium isolated from oil-polluted soil was identified as Microbacterium sp. F10a based on 16S rDNA gene sequence analysis. Plant growth promoting characteristics of the strain, degradation rate of phenanthrene and pyrene, and cell surface hydrophobicity characteristics of the strain were further characterized. The strain was also evaluated for promoting the growth of wheat and phenanthrene and pyrene removal from soil artificially contaminated with a mixture of phenanthrene (200 mg·kg–1) and pyrene (150 mg·kg–1) in pot experiments. The strain had the plant growth promoting characteristics of producing indole acetic acid, siderophore, and 1-aminocyclopropane-1-carboxylate deaminase activity and solubilizing inorganic phosphate. The strain also has a cell surface hydrophobicity that could increase the aqueous polycyclic aromatic hydrocarbon solubility. High-performance liquid chromatographic analysis showed that the degradation rates of phenanthrene (50 mg·L–1) and pyrene (20 mg·L–1) were 98% and 65%, respectively, under 28 °C after 7 days. Inoculation with the strain was found to significantly increase (p < 0.05) the growth of wheat and phenanthrene and pyrene removal in the unplanted or planted soils in a low-temperature environment. There were no significant differences in culturable bacterial numbers between live bacterial inoculation and dead bacterial inoculation controls in the unplanted and planted soils. However, the numbers of polycyclic aromatic hydrocarbon degrading bacteria were significantly greater in the inoculated planted or unplanted soils compared with the dead bacterial inoculation controls.

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