scholarly journals Bacterial cell surface hydrophobicity properties in the mediation of in vitro adhesion by the rabbit enteric pathogen Escherichia coli strain RDEC-1.

1989 ◽  
Vol 84 (5) ◽  
pp. 1588-1594 ◽  
Author(s):  
B Drumm ◽  
A W Neumann ◽  
Z Policova ◽  
P M Sherman
2020 ◽  
Vol 21 (15) ◽  
pp. 5548
Author(s):  
Zhiliang Yu ◽  
Zeyu Hu ◽  
Qimiao Xu ◽  
Mengting Zhang ◽  
Nate Yuan ◽  
...  

Members of the Sphingomonadales are renowned for their ability to degrade polycyclic aromatic hydrocarbons (PAHs). However, little is known about the regulatory mechanisms of the degradative pathway. Using cross-feeding bioassay, a functional LuxI/LuxR-type acyl-homoserine lactone (AHL)-mediated quorum sensing (QS) system was identified from Croceicoccus naphthovorans PQ-2, a member of the order Sphingomonadales. Inactivation of the QS system resulted in a significant decrease in PAHs degradation. The QS system positively controlled the expression of three PAH-degrading genes (ahdA1e, xylE and xylG) and a regulatory gene ardR, which are located on the large plasmid. Interestingly, the transcription levels of these three PAH-degrading genes were significantly down-regulated in the ardR mutant. In addition, bacterial cell surface hydrophobicity and cell morphology were altered in the QS-deficient mutant. Therefore, the QS system in strain PQ-2 positively regulates PAH degradation via two mechanisms: (i) by induction of PAH-degrading genes directly and/or indirectly; and (ii) by an increase of bacterial cell surface hydrophobicity. The findings of this study improve our understanding of how the QS system influences the degradation of PAHs, therefore facilitating the development of new strategies for the bioremediation of PAHs.


1985 ◽  
Vol 30 (1-2) ◽  
pp. 131-138 ◽  
Author(s):  
Ferenc Rozgonyi ◽  
Katalin R. Szitha ◽  
Ã…sa Ljungh ◽  
Suraj B. Baloda ◽  
Stellan Hjertén ◽  
...  

2016 ◽  
Vol 25 ◽  
pp. 367-374 ◽  
Author(s):  
Stefan W. Wessel ◽  
Henny C. van der Mei ◽  
Anje M. Slomp ◽  
Betsy van de Belt-Gritter ◽  
Amarnath Maitra ◽  
...  

Author(s):  
Jesús A. Salas-Tovar ◽  
Sarai Escobedo-García ◽  
Guadalupe I. Olivas ◽  
Carlos H. Acosta-Muñiz ◽  
Federico Harte ◽  
...  

2004 ◽  
Vol 56 (7) ◽  
pp. 847-854 ◽  
Author(s):  
G. W. Hanlon ◽  
S. P. Denyer ◽  
N. A. Hodges ◽  
J. A. Brant ◽  
A. B. Lansley ◽  
...  

Author(s):  
Ferenc Rozgonyi ◽  
Åsa Ljungh ◽  
Wubshet Mamo ◽  
Stellan Hjertén ◽  
Torkel Wadström

Author(s):  
Kamni Rajput ◽  
Ramesh Chandra Dubey

In this paper, an investigation on lactic acid bacterial isolates from ethnic goat raw milk samples were examined for their probiotic potential and safety parameters. For this purpose, isolated bacterial cultures were screened based on certain parameters viz., sugar fermentation, tolerance to temperature, salt, low pH, bile salts, and phenol resistance. After that, these bacterial cultures were more estimated in vitro for auto-aggregation, cell surface hydrophobicity, response to simulated stomach duodenum channel, antibiotic resistance, and antimicrobial activity. Besides, probiotic traits show the absence of gelatinase and hemolytic activity supports its safety. The isolate G24 showed good viability at different pH, bile concentration, phenol resistance and response to simulated stomach duodenum passage but it did not show gelatinase and hemolytic activities. Isolate G24 was susceptible to amikacin, carbenicillin, kanamycin, ciprofloxacin, co-trimazine, nitrofurantoin, streptomycin, and tetracycline. Isolate G24 also exhibited antimicrobial action against five common pathogenic bacteria, such as Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Listeria monocytogens, and Salmonella typhimurium. It displayed the maximum auto-aggregation, cell surface hydrophobicity to different hydrocarbons. Following molecular characterization the isolate G24 was identified as Enterococcus hirae with 16S rRNA gene sequencing and phylogeny. E. hirae G24 bears the excellent properties of probiotics.


1999 ◽  
Vol 62 (3) ◽  
pp. 252-256 ◽  
Author(s):  
C. GUSILS ◽  
A. PÉREZ CHAIA ◽  
S. GONZÁLEZ ◽  
G. OLIVER

Lactobacillus strains were tested for their in vitro probiotic properties. Cell surface hydrophobicity was found to be very high for Lactobacillus fermentum subsp. cellobiosus and Salmonella Gallinarum; high values could indicate a greater ability to adhere to epithelial cells. Studies on Lactobacillus animalis indicated relative cell surface hydrophobicities smaller than those of L. fermentum subsp. cellobiosus and L. fermentum. L. animalis and Enterococcus faecalis were able to coaggregate with L. fermentum subsp. cellobiosus and L. fermentum, respectively, but not with Salmonella Gallinarum. After mixed-culture studies for determining suitable growth behavior, the pair of strains L. animalis plus L. fermentum subsp. cellobiosus was selected for an attempted challenge against Salmonella Gallinarum. Double and triple mixed-culture studies indicated that selected lactobacillus strains were able to retain their beneficial characteristics in the presence of Salmonella Gallinarum such as presence of lectins, production of antimicrobial compounds, and ability to grow and compete. The selected microorganisms can be considered as potential ingredients for a chicken probiotic feed formulation intended to control salmonellosis and also improve poultry sanitation.


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