scholarly journals A First Glimpse of the Mexican Fruit Fly Anastrepha ludens (Diptera: Tephritidae) Antenna Morphology and Proteome in Response to a Proteinaceous Attractant

2020 ◽  
Vol 21 (21) ◽  
pp. 8086
Author(s):  
Eliel Ruiz-May ◽  
Alma Altúzar-Molina ◽  
José M. Elizalde-Contreras ◽  
Jiovanny Arellano-de los Santos ◽  
Juan Monribot-Villanueva ◽  
...  
Keyword(s):  
Odorant Receptor ◽  
Management Strategies ◽  
Fruit Fly ◽  
Anastrepha Ludens ◽  
Mexican Fruit Fly ◽  
Odor Perception ◽  
Related Proteins ◽  
Odorant Binding ◽  
Odor Receptors ◽  
Specific Odor

Anastrepha ludens is a key pest of mangoes and citrus from Texas to Costa Rica but the mechanisms of odorant perception in this species are poorly understood. Detection of volatiles in insects occurs mainly in the antenna, where molecules penetrate sensillum pores and link to soluble proteins in the hemolymph until reaching specific odor receptors that trigger signal transduction and lead to behavioral responses. Scrutinizing the molecular foundation of odorant perception in A. ludens is necessary to improve biorational management strategies against this pest. After exposing adults of three maturity stages to a proteinaceous attractant, we studied antennal morphology and comparative proteomic profiles using nano-LC-MS/MS with tandem mass tags combined with synchronous precursor selection (SPS)-MS3. Antennas from newly emerged flies exhibited dense agglomerations of olfactory sensory neurons. We discovered 4618 unique proteins in the antennas of A. ludens and identified some associated with odor signaling, including odorant-binding and calcium signaling related proteins, the odorant receptor co-receptor (Orco), and putative odorant-degrading enzymes. Antennas of sexually immature flies exhibited the most upregulation of odor perception proteins compared to mature flies exposed to the attractant. This is the first report where critical molecular players are linked to the odor perception mechanism of A. ludens.

Survival ability of Mexican fruit fly males from different strains in presence of the predatory orb-weaving spider Argiope argentata (Araneae: Araneidae)

2018 ◽  
Vol 109 (3) ◽  
pp. 279-286
Author(s):  
A. Dor ◽  
P. Liedo
Keyword(s):  
Integrated Management ◽  
Management Strategies ◽  
Fruit Fly ◽  
Mass Rearing ◽  
Anastrepha Ludens ◽  
Mexican Fruit Fly ◽  
Colony Management ◽  
Rearing Conditions ◽  
Negative Effect ◽  
Different Strains

AbstractThe sterile insect technique (SIT) is a key element for the integrated management of pest populations of the Mexican fruit fly, Anastrepha ludens, in Mexico. Its success depends on the survival of mass-reared sterile males and their ability to mate with wild females. However, colonization and mass-rearing conditions can adversely affect their ability to avoid predators. To test if colony management strategies could contribute to improve field survival abilities of mass-reared flies, we compared the survival of males exposed to the orb-weaver spider Argiope argentata. Males compared originated from three strains with different colonization strategies: (a) a colony started from field-collected wild flies (replacement), (b) a colony started by hybridizing wild males with mass-reared adapted females (hybrid) and (c) a colony started with mass-reared males selected on the basis of their survival ability and mating competitiveness in field cages (selected). Mass-reared males and wild males were used as controls. Males were exposed to spiders under laboratory cage conditions. Overall, wild males showed better survival ability than mass-reared males. Regarding the colonization approach, wild males survived better than a hybrid, replaced and selected males. We conclude that mass-rearing conditions have a strong negative effect on the ability of males to escape spiders. The colonization systems evaluated did not counter this effect. The lower survival of males from the selected colony suggests that the selection over one generation did not contribute to improve males’ predator avoidance and escape abilities and probably needs to be modified. Possible explanations for this and implications on colonization and colony management for SIT purpose are discussed.

scholarly journals Caribbean Fruit Fly, Anastrepha suspensa (Loew) (Insecta: Diptera: Tephritidae)

EDIS ◽  
1969 ◽  
Vol 2004 (5) ◽  
Author(s):  
Howard V. Weems, Jr. ◽  
John B. Heppner ◽  
Thomas R. Fasulo ◽  
James L. Nation
Keyword(s):  
Fruit Fly ◽  
Cooperative Extension Service ◽  
Anastrepha Ludens ◽  
Anastrepha Suspensa ◽  
Mexican Fruit Fly ◽  
The West ◽  
University Of Florida ◽  
Caribbean Fruit Fly ◽  
Guava Fruit ◽  
The Caribbean

The Caribbean fruit fly, Anastrepha suspensa (Loew), has also been called the Greater Antilliean fruit fly, the guava fruit fly and the Caribfly. It is a near relative of the Mexican fruit fly, Anastrepha ludens (Loew), and is one of several species of fruit flies which are indigenous to the West Indies and the larvae of which attack several kinds of tropical and subtropical fruits. This document is EENY-196 (originally published as DPI Entomology Circulars 38 and 260), one of a series of Featured Creatures from the Entomology and Nematology Department, Florida Cooperative Extension Service, Institute of Food and Agricultural Sciences, University of Florida. Published: March 2001. EENY196/IN353: Caribbean Fruit Fly, Anastrepha suspensa (Loew) (Insecta: Diptera: Tephritidae) (ufl.edu)

scholarly journals Host Marking Pheromone (HMP) in the Mexican Fruit Fly Anastrepha ludens

2010 ◽  
Vol 64 (1) ◽  
pp. 37-42 ◽  
Author(s):  
Andrew J. F. Edmunds ◽  
Martin Aluja ◽  
Fransico Diaz-Fleischer ◽  
Bruno Patrian ◽  
Leonhard Hagmann
Keyword(s):  
Fruit Fly ◽  
Anastrepha Ludens ◽  
Mexican Fruit Fly ◽  
Marking Pheromone ◽  
Host Marking Pheromone

scholarly journals Genetic and Ecological Relationships of Anastrepha ludens (Diptera: Tephritidae) Populations in Southern Mexico

Insects ◽  
2020 ◽  
Vol 11 (11) ◽  
pp. 815
Author(s):  
Lorena Ruiz-Montoya ◽  
Rodrigo Verónica Vallejo ◽  
David Haymer ◽  
Pablo Liedo
Keyword(s):  
Sexual Selection ◽  
Genetic Differentiation ◽  
Host Plants ◽  
Control Strategies ◽  
Mangifera Indica ◽  
Random Mating ◽  
Management Strategies ◽  
Fruit Fly ◽  
Anastrepha Ludens ◽  
Southern Mexico

Knowledge of the influence of evolutionary factors that promote either the differentiation or cohesion of pest insect populations is critical for the improvement of control strategies. Here, we explore the extent to which genetic differentiation occurs between populations of the Mexican fruit fly, Anastrepha ludens, in association with four plant hosts (Citrus sinensis, C. paradisi, Mangifera indica and Casimiroa edulis) in the Soconusco region of Chiapas (Mexico). Using variants from six enzymatic loci, we obtained measures of genetic diversity for three sample arrangements: (1) by sex per locality, (2) by locality and (3) by host. The extent of genetic differentiation in populations was assessed using the Analyses of Molecular Variance (AMOVA) method for each array of samples, and moderate to high levels of genetic variation were observed between the sexes, as well as among localities and host plants. A Bayesian approach was then used to assess any population structure underlying the genetic data we obtained, but this analysis showed no significant structuring due to locality or host plant. We also considered whether the observed genotypic frequencies in male and females matched those expected under a hypothesis of random mating. Here we found significant deviations from expected genotypic frequencies, suggesting that sexual selection is acting on these populations. Overall, our results indicate that sexual selection, along with the presence of some heterogeneity in environments provided by both geographical factors and availability of host plants, has influenced the evolution of pest populations in this region of Mexico. Implications for area-wide pest management strategies are discussed.

Superparasitism strategies by a native and an exotic parasitoid species attacking the Mexican fruit fly,Anastrepha ludens(Diptera: Tephritidae)

2014 ◽  
Vol 24 (8) ◽  
pp. 925-935 ◽  
Author(s):  
Amanda Ayala ◽  
Ana Mabel Martinez ◽  
Isaac Figueroa ◽  
Samuel Pineda ◽  
Mario Miranda ◽  
...  
Keyword(s):  
Fruit Fly ◽  
Anastrepha Ludens ◽  
Mexican Fruit Fly ◽  
Parasitoid Species

Comparisons of Cobalt-60 Gamma Irradiation Dose Rates on Grapefruit Flavedo Tissue and on Mexican Fruit Fly Mortality

1990 ◽  
Vol 53 (4) ◽  
pp. 329-331 ◽  
Author(s):  
GENE E. LESTER ◽  
DAN A. WOLFENBARGER
Keyword(s):  
Gamma Irradiation ◽  
Dose Rate ◽  
Electrolyte Leakage ◽  
Irradiation Dose ◽  
Adult Emergence ◽  
Fruit Fly ◽  
Anastrepha Ludens ◽  
Mexican Fruit Fly ◽  
Dose Rates ◽  
Grapefruit Peel

Percent electrolyte leakage, a measure of membrane integrity, proved to be a good predictor (R2 = 0.99) of cobalt-60 gamma irradiation dose injury on mid-season ‘Ruby Red’ grapefruit Citrus paradisi (Macf.) flavedo tissue (peel). Percent electrolyte leakage on grapefruit peel following a dose-rate of 250 grays/1.0, 2.5, 5.0, and 25.0 min decreased as grays/min (dose-rate) decreased. Total phenols, a biochemical response to irradiation following 250 grays/1.0, 2.5, 5.0, and 25.0 min also decreased as dose-rate decreased, demonstrating that injury to grapefruit peel diminished as 250 grays of gamma irradiation/rate declined. Comparisons of 10 and 20 grays of cobalt-60 gamma irradiation showed that a dose-rate of 10 grays/0.25 min to naked 8-d old Mexican fruit fly Anastrepha ludens (Loew) larvae caused a 90% reduction of adult emergence. Whereas, at 20 grays the reduction was greater than 99% with dose-rates of 20 grays/0.25, 0.5, 1.0, or 100 min. These data show that a gamma irradiation dose capable of reducing fly emergence by >99% will maintain an inhibitory effect even at relatively lower dose-rates. Therefore, once a quarantine security treatment for Mexican fruit fly is established, a lower dose-rate will reduce adult emergence and should impart little damage to grapefruit peel tissue.

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