scholarly journals Changes in Phenotypes and DNA Methylation of In Vitro Aging Sperm in Common Carp Cyprinus carpio

2021 ◽  
Vol 22 (11) ◽  
pp. 5925
Author(s):  
Yu Cheng ◽  
Pavlina Vechtova ◽  
Zoltan Fussy ◽  
Jan Sterba ◽  
Zuzana Linhartová ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Common Carp ◽  
Cyprinus Carpio ◽  
Seminal Fluid ◽  
Computer Assisted ◽  
Hatching Rate ◽  
Common Carp Cyprinus Carpio ◽  
In Vitro Aging ◽  
Carp Cyprinus Carpio

The purpose of the current study was to analyze phenotypic and functional characteristics of common carp (Cyprinus carpio) spermatozoa during in vitro aging and to investigate whether global DNA methylation is affected by sperm aging. Milt was collected from five individual males, stored in vitro on ice in a refrigerator for up to 96 h post stripping (HPS) and used to fertilize eggs with intervals of 1, 24 and 96 h. Computer-assisted sperm analysis and a S3e Cell Sorter was employed to determine the spermatozoa phenotypic characteristics (motility, velocity, concentration and viability). In addition, pH and osmolality of the seminal fluid and the capacity of the spermatozoa to fertilize, hatching rate and health of the resulting embryos were examined at different aging times. Whole-genome bisulfite sequencing was used to compare the global and gene-specific DNA methylation in fresh and aged spermatozoa. The results demonstrated that spermatozoa aging in common carp significantly affects their performance and thus the success of artificial fertilization. The methylation level at the cytosine-phosphate-guanine (CpG) sites increased significantly with 24 HPS spermatozoa compared to the fresh group at 1 HPS and then decreased significantly at 96 HPS. A more detailed investigation of gene specific differences in the DNA methylation was hindered by incomplete annotation of the C. carpio genome in the public databases.

scholarly journals The in vitro toxicity of atrazine on kinematics and DNA fragmentation in common carp (Cyprinus carpio) sperm cells

2019 ◽  
Vol 70 (3) ◽  
pp. 1639
Author(s):  
M.E. ÖZGÜR
Keyword(s):  
Dna Fragmentation ◽  
Common Carp ◽  
Cyprinus Carpio ◽  
In Vitro Toxicity ◽  
Computer Assisted ◽  
Sperm Cells ◽  
Common Carp Cyprinus Carpio ◽  
Significant Difference ◽  
Carp Cyprinus Carpio

This study investigated the in vitro effects of different concentrations of Atrazine (0.001, 0.01, 0.1, 0.5 mg/L) added to motile and immotile solutions on kinematics quality of sperm cells of common carp, Cyprinus carpio, which is a fish of economic significance. The kinematics of the sperm cells was analyzed by a computer-assisted sperm analysis system (CASA). As a result of the study, while there was a significant difference (P < 0.05) between the groups in terms of the VSL (μm/s) and VCL (μm/s) values after the Atrazine-added immotile solution’s (IMS) and incubation for 3 hours, there was a significant difference (P < 0.05) in only the VSL values directly activated by the Atrazine-added motile solution (MS). DNA fragmentation was evident but not in higher numbers in the 0.1 mg/L atrazine group. Finally, it was determined the effective concentration (EC50) values of the VSL value of the motile and immotile solution as 0.34 mg/L and 0.03 mg/L, respectively.

scholarly journals In vitro cultivation of Trypanoplasma borreli (Protozoa:Kinetoplastida), a parasite from the blood of common carp Cyprinus carpio

2000 ◽  
Vol 41 ◽  
pp. 195-201 ◽  
Author(s):  
D Steinhagen ◽  
W Hedderich ◽  
A Skouras ◽  
JP Scharsack ◽  
J Schuberth ◽  
...  
Keyword(s):  
Common Carp ◽  
Cyprinus Carpio ◽  
In Vitro Cultivation ◽  
Common Carp Cyprinus Carpio ◽  
Carp Cyprinus Carpio
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