scholarly journals One Hundred Candidate Genes and Their Roles in Drought and Salt Tolerance in Wheat

2021 ◽  
Vol 22 (12) ◽  
pp. 6378
Author(s):  
Ieva Urbanavičiūtė ◽  
Luca Bonfiglioli ◽  
Mario A. Pagnotta
Keyword(s):  
Redox Homeostasis ◽  
Climatic Changes ◽  
Main Function ◽  
Sources Of Resistance ◽  
Future Studies ◽  
Drought And Salt Tolerance ◽  
Drought And Salt Stress ◽  
Hydric Stress ◽  
Efficient Selection ◽  
Selection Of

Drought and salinity are major constraints to agriculture. In this review, we present an overview of the global situation and the consequences of drought and salt stress connected to climatic changes. We provide a list of possible genetic resources as sources of resistance or tolerant traits, together with the previous studies that focused on transferring genes from the germplasm to cultivated varieties. We explained the morphological and physiological aspects connected to hydric stresses, described the mechanisms that induce tolerance, and discussed the results of the main studies. Finally, we described more than 100 genes associated with tolerance to hydric stresses in the Triticeae. These were divided in agreement with their main function into osmotic adjustment and ionic and redox homeostasis. The understanding of a given gene function and expression pattern according to hydric stress is particularly important for the efficient selection of new tolerant genotypes in classical breeding. For this reason, the current review provides a crucial reference for future studies on the mechanism involved in hydric stress tolerance and the use of these genes in mark assistance selection (MAS) to select the wheat germplasm to face the climatic changes.

scholarly journals Network Analysis Based on Unique Spectral Features Enables an Efficient Selection of Genomically Diverse Operational Isolation Units

2021 ◽  
Vol 9 (2) ◽  
pp. 416
Author(s):  
Charles Dumolin ◽  
Charlotte Peeters ◽  
Evelien De Canck ◽  
Nico Boon ◽  
Peter Vandamme
Keyword(s):  
Network Analysis ◽  
Hierarchical Clustering ◽  
Mass Spectra ◽  
Spectral Features ◽  
Maldi Tof ◽  
Maldi Tof Mass Spectra ◽  
Diversity Studies ◽  
Technical Sample ◽  
Efficient Selection ◽  
Selection Of

Culturomics-based bacterial diversity studies benefit from the implementation of MALDI-TOF MS to remove genomically redundant isolates from isolate collections. We previously introduced SPeDE, a novel tool designed to dereplicate spectral datasets at an infraspecific level into operational isolation units (OIUs) based on unique spectral features. However, biological and technical variation may result in methodology-induced differences in MALDI-TOF mass spectra and hence provoke the detection of genomically redundant OIUs. In the present study, we used three datasets to analyze to which extent hierarchical clustering and network analysis allowed to eliminate redundant OIUs obtained through biological and technical sample variation and to describe the diversity within a set of spectra obtained from 134 unknown soil isolates. Overall, network analysis based on unique spectral features in MALDI-TOF mass spectra enabled a superior selection of genomically diverse OIUs compared to hierarchical clustering analysis and provided a better understanding of the inter-OIU relationships.

Efficient selection of feature sets possessing high coefficients of determination based on incremental determinations

2003 ◽  
Vol 83 (4) ◽  
pp. 695-712 ◽  
Author(s):  
Ronaldo F. Hashimoto ◽  
Edward.R. Dougherty ◽  
Marcel Brun ◽  
Zheng-Zheng Zhou ◽  
Michael L. Bittner ◽  
...  
Keyword(s):  
Feature Sets ◽  
Efficient Selection ◽  
Selection Of

scholarly journals A Dual-Sensor-Based Screening System for In Vitro Selection of TDP1 Inhibitors

Sensors ◽  
2021 ◽  
Vol 21 (14) ◽  
pp. 4832
Author(s):  
Ann-Katrine Jakobsen ◽  
Josephine Geertsen Keller ◽  
María Gonzalez ◽  
Endika Martin-Encinas ◽  
Francisco Palacios ◽  
...  
Keyword(s):  
Dna Damage ◽  
In Vitro Selection ◽  
Main Function ◽  
Screening System ◽  
Drug Candidates ◽  
Topoisomerase 1 ◽  
High Throughput Drug Screening ◽  
Dual Sensor ◽  
Selection Of

DNA sensors can be used as robust tools for high-throughput drug screening of small molecules with the potential to inhibit specific enzymes. As enzymes work in complex biological pathways, it is important to screen for both desired and undesired inhibitory effects. We here report a screening system utilizing specific sensors for tyrosyl-DNA phosphodiesterase 1 (TDP1) and topoisomerase 1 (TOP1) activity to screen in vitro for drugs inhibiting TDP1 without affecting TOP1. As the main function of TDP1 is repair of TOP1 cleavage-induced DNA damage, inhibition of TOP1 cleavage could thus reduce the biological effect of the TDP1 drugs. We identified three new drug candidates of the 1,5-naphthyridine and 1,2,3,4-tetrahydroquinolinylphosphine sulfide families. All three TDP1 inhibitors had no effect on TOP1 activity and acted synergistically with the TOP1 poison SN-38 to increase the amount of TOP1 cleavage-induced DNA damage. Further, they promoted cell death even with low dose SN-38, thereby establishing two new classes of TDP1 inhibitors with clinical potential. Thus, we here report a dual-sensor screening approach for in vitro selection of TDP1 drugs and three new TDP1 drug candidates that act synergistically with TOP1 poisons.

Cost-efficient selection of a marker panel in genetic studies

2011 ◽  
Vol 76 (1) ◽  
pp. 88-94 ◽  
Author(s):  
Jamie S. Sanderlin ◽  
Nicole Lazar ◽  
Michael J. Conroy ◽  
Jaxk Reeves
Keyword(s):  
Genetic Studies ◽  
Marker Panel ◽  
Cost Efficient ◽  
Efficient Selection ◽  
Selection Of
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