scholarly journals Coumarin Interferes with Polar Auxin Transport Altering Microtubule Cortical Array Organization in Arabidopsis thaliana (L.) Heynh. Root Apical Meristem

2021 ◽  
Vol 22 (14) ◽  
pp. 7305
Author(s):  
Leonardo Bruno ◽  
Emanuela Talarico ◽  
Luz Cabeiras-Freijanes ◽  
Maria Letizia Madeo ◽  
Antonella Muto ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Lateral Root ◽  
Apical Meristem ◽  
Root Formation ◽  
Auxin Transport ◽  
Primary Root ◽  
Polar Auxin Transport ◽  
Root Apical Meristem ◽  
Lateral Root Formation ◽  
Cortical Array

Coumarin is a phytotoxic natural compound able to affect plant growth and development. Previous studies have demonstrated that this molecule at low concentrations (100 µM) can reduce primary root growth and stimulate lateral root formation, suggesting an auxin-like activity. In the present study, we evaluated coumarin’s effects (used at lateral root-stimulating concentrations) on the root apical meristem and polar auxin transport to identify its potential mode of action through a confocal microscopy approach. To achieve this goal, we used several Arabidopsis thaliana GFP transgenic lines (for polar auxin transport evaluation), immunolabeling techniques (for imaging cortical microtubules), and GC-MS analysis (for auxin quantification). The results highlighted that coumarin induced cyclin B accumulation, which altered the microtubule cortical array organization and, consequently, the root apical meristem architecture. Such alterations reduced the basipetal transport of auxin to the apical root apical meristem, inducing its accumulation in the maturation zone and stimulating lateral root formation.

scholarly journals AT-HOOK MOTIF NUCLEAR LOCALISED PROTEIN 18 as a Novel Modulator of Root System Architecture

2020 ◽  
Author(s):  
Marek Šírl ◽  
Tereza Šnajdrová ◽  
Dolores Gutiérrez-Alanís ◽  
Joseph G. Dubrovsky ◽  
Jean Phillipe Vielle-Calzada ◽  
...  
Keyword(s):  
Root System ◽  
System Architecture ◽  
Lateral Root ◽  
Apical Meristem ◽  
Lateral Roots ◽  
Primary Root ◽  
Root System Architecture ◽  
Root Apical Meristem ◽  
Root Initiation ◽  
Lateral Root Initiation

The AT-HOOK MOTIF NUCLEAR LOCALIZED PROTEIN (AHL) gene family encodes embryophyte-specific nuclear proteins with DNA binding activity. They modulate gene expression and affect various developmental processes in plants. We identify AHL18 (At3G60870) as a developmental modulator of root system architecture and growth. AHL18 regulates the length of the proliferation domain and number of dividing cells in the root apical meristem and thereby, cell production. Both primary root growth and lateral root development respond according to AHL18 transcription level. The ahl18 knock-out plants show reduced root systems due to a shorter primary root and a lower number of lateral roots. This change results from a higher number of arrested and non-developing lateral root primordia (LRP) rather than from decreased initiation. Overexpression of AHL18 results in a more extensive root system, longer primary roots, and increased density of lateral root initiation events. Formation of lateral roots is affected during the initiation of LRP and later development. AHL18 regulate root apical meristem activity, lateral root initiation and emergence, which is in accord with localization of its expression.

scholarly journals Control of auxin-regulated root development by the Arabidopsis thaliana SHY2/IAA3 gene

Development ◽  
1999 ◽  
Vol 126 (4) ◽  
pp. 711-721 ◽  
Author(s):  
Q. Tian ◽  
J.W. Reed
Keyword(s):  
Arabidopsis Thaliana ◽  
Lateral Root ◽  
Tissue Specificity ◽  
Plant Hormone ◽  
Root Formation ◽  
Auxin Transport ◽  
Feedback Regulation ◽  
Loss Of Function ◽  
Leaf Formation ◽  
Induced Genes

The plant hormone auxin controls many aspects of development and acts in part by inducing expression of various genes. Arabidopsis thaliana semidominant shy2 (short hypocotyl) mutations cause leaf formation in dark-grown plants, suggesting that SHY2 has an important role in regulating development. Here we show that the SHY2 gene encodes IAA3, a previously known member of the Aux/IAA family of auxin-induced genes. Dominant shy2 mutations cause amino acid changes in domain II, conserved among all members of this family. We isolated loss-of-function shy2 alleles including a putative null mutation. Gain-of-function and loss-of-function shy2 mutations affect auxin-dependent root growth, lateral root formation, and timing of gravitropism, indicating that SHY2/IAA3 regulates multiple auxin responses in roots. The phenotypes suggest that SHY2/IAA3 may activate some auxin responses and repress others. Models invoking tissue-specificity, feedback regulation, or control of auxin transport may explain these results.

scholarly journals Arabidopsis thaliana RALF1 opposes brassinosteroid effects on root cell elongation and lateral root formation

2014 ◽  
Vol 65 (8) ◽  
pp. 2219-2230 ◽  
Author(s):  
Tábata Bergonci ◽  
Bianca Ribeiro ◽  
Paulo H.O. Ceciliato ◽  
Juan Carlos Guerrero-Abad ◽  
Marcio C. Silva-Filho ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Cell Elongation ◽  
Lateral Root ◽  
Root Formation ◽  
Root Cell ◽  
Lateral Root Formation

scholarly journals LBD29 regulates the cell cycle progression in response to auxin during lateral root formation in Arabidopsis thaliana

2012 ◽  
Vol 110 (1) ◽  
pp. 1-10 ◽  
Author(s):  
Zhenhua Feng ◽  
Xudong Sun ◽  
Guangchao Wang ◽  
Hailiang Liu ◽  
Jian Zhu
Keyword(s):  
Cell Cycle ◽  
Arabidopsis Thaliana ◽  
Lateral Root ◽  
Cell Cycle Progression ◽  
Root Formation ◽  
Lateral Root Formation ◽  
Cycle Progression

scholarly journals The cyclophilin A DIAGEOTROPICA gene affects auxin transport in both root and shoot to control lateral root formation

Development ◽  
2015 ◽  
Vol 142 (4) ◽  
pp. 712-721 ◽  
Author(s):  
M. G. Ivanchenko ◽  
J. Zhu ◽  
B. Wang ◽  
E. Medvecka ◽  
Y. Du ◽  
...  
Keyword(s):  
Lateral Root ◽  
Root Formation ◽  
Auxin Transport ◽  
Cyclophilin A ◽  
Lateral Root Formation

scholarly journals Chrysanthemum transcription factor CmLBD1 direct lateral root formation in Arabidopsis thaliana

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Lu Zhu ◽  
Chen Zheng ◽  
Ruixia Liu ◽  
Aiping Song ◽  
Zhaohe Zhang ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Transcription Factor ◽  
Lateral Root ◽  
Root Formation ◽  
Lateral Root Formation

scholarly journals Ethylene regulates lateral root formation and auxin transport inArabidopsis thaliana

2008 ◽  
Vol 55 (2) ◽  
pp. 175-187 ◽  
Author(s):  
Sangeeta Negi ◽  
Maria G. Ivanchenko ◽  
Gloria K. Muday
Keyword(s):  
Lateral Root ◽  
Root Formation ◽  
Auxin Transport ◽  
Lateral Root Formation
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