scholarly journals R2R3-MYB Transcription Factor SmMYB52 Positively Regulates Biosynthesis of Salvianolic Acid B and Inhibits Root Growth in Salvia miltiorrhiza

2021 ◽  
Vol 22 (17) ◽  
pp. 9538
Author(s):  
Rao Yang ◽  
Shengsong Wang ◽  
Haolan Zou ◽  
Lin Li ◽  
Yonghui Li ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Root Growth ◽  
Phenolic Acid ◽  
Salvia Miltiorrhiza ◽  
Salvianolic Acid B ◽  
Myb Transcription Factor ◽  
Acid Biosynthesis ◽  
Key Enzyme ◽  
R2r3 Myb ◽  
Encoding Genes

The dried root of Salvia miltiorrhiza is a renowned traditional Chinese medicine that was used for over 1000 years in China. Salvianolic acid B (SalB) is the main natural bioactive product of S. miltiorrhiza. Although many publications described the regulation mechanism of SalB biosynthesis, few reports simultaneously focused on S. miltiorrhiza root development. For this study, an R2R3-MYB transcription factor gene (SmMYB52) was overexpressed and silenced, respectively, in S. miltiorrhiza sterile seedlings. We found that SmMYB52 significantly inhibited root growth and indole-3-acetic acid (IAA) accumulation, whereas it activated phenolic acid biosynthesis and the jasmonate acid (JA) signaling pathway. Quantitative real-time polymerase chain reaction (qRT-PCR) analyses revealed that SmMYB52 suppressed the transcription levels of key enzyme-encoding genes involved in the IAA biosynthetic pathway and activated key enzyme-encoding genes involved in the JA and phenolic acid biosynthesis pathways. In addition, yeast one-hybrid (Y1H) and dual-luciferase assay showed that SmMYB52 directly binds to and activates the promoters of several key enzyme genes for SalB biosynthesis, including SmTAT1, Sm4CL9, SmC4H1, and SmHPPR1, to promote the accumulation of SalB. This is the first report of a regulator that simultaneously affects root growth and the production of phenolic acids in S. miltiorrhiza.

scholarly journals The methyl jasmonate-responsive transcription factor SmMYB1 promotes phenolic acid biosynthesis in Salvia miltiorrhiza

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Wei Zhou ◽  
Min Shi ◽  
Changping Deng ◽  
Sunjie Lu ◽  
Fenfen Huang ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Methyl Jasmonate ◽  
Phenolic Acids ◽  
Phenolic Acid ◽  
Salvia Miltiorrhiza ◽  
Water Soluble ◽  
Biosynthesis Pathway ◽  
Acid Biosynthesis ◽  
Expression Of Genes ◽  
Genes Encoding

AbstractWater-soluble phenolic acids are major bioactive compounds in the medicinal plant species Salvia miltiorrhiza. Phenolic acid biosynthesis is induced by methyl jasmonate (MeJA) in this important Chinese herb. Here, we investigated the mechanism underlying this induction by analyzing a transcriptome library of S. miltiorrhiza in response to MeJA. Global transcriptome analysis identified the MeJA-responsive R2R3-MYB transcription factor-encoding gene SmMYB1. Overexpressing SmMYB1 significantly promoted phenolic acid accumulation and upregulated the expression of genes encoding key enzymes in the phenolic acid biosynthesis pathway, including cytochrome P450-dependent monooxygenase (CYP98A14). Dual-luciferase (dual-LUC) assays and/or an electrophoretic mobility shift assays (EMSAs) indicated that SmMYB1 activated the expression of CYP98A14, as well as the expression of genes encoding anthocyanin biosynthesis pathway enzymes, including chalcone isomerase (CHI) and anthocyanidin synthase (ANS). In addition, SmMYB1 was shown to interact with SmMYC2 to additively promote CYP98A14 expression compared to the action of SmMYB1 alone. Taken together, these results demonstrate that SmMYB1 is an activator that improves the accumulation of phenolic acids and anthocyanins in S. miltiorrhiza. These findings lay the foundation for in-depth studies of the molecular mechanism underlying MeJA-mediated phenolic acid biosynthesis and for the metabolic engineering of bioactive ingredients in S. miltiorrhiza.

scholarly journals An R2R3 MYB transcription factor confers brown planthopper resistance by regulating the phenylalanine ammonia-lyase pathway in rice

2019 ◽  
Vol 117 (1) ◽  
pp. 271-277 ◽  
Author(s):  
Jun He ◽  
Yuqiang Liu ◽  
Dingyang Yuan ◽  
Meijuan Duan ◽  
Yanling Liu ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Phenylalanine Ammonia Lyase ◽  
Brown Planthopper ◽  
Rice Cultivar ◽  
Phenylpropanoid Pathway ◽  
Myb Transcription Factor ◽  
Resistance Response ◽  
Key Enzyme ◽  
R2r3 Myb

Brown planthopper (BPH) is one of the most destructive insects affecting rice (Oryza sativaL.) production. Phenylalanine ammonia-lyase (PAL) is a key enzyme involved in plant defense against pathogens, but the role of PAL in insect resistance is still poorly understood. Here we show that expression of the majority ofPALsin rice is significantly induced by BPH feeding. Knockdown of OsPALssignificantly reduces BPH resistance, whereas overexpression ofOsPAL8in a susceptible rice cultivar significantly enhances its BPH resistance. We found thatOsPALsmediate resistance to BPH by regulating the biosynthesis and accumulation of salicylic acid and lignin. Furthermore, we show that expression ofOsPAL6andOsPAL8in response to BPH attack is directly up-regulated by OsMYB30, an R2R3 MYB transcription factor. Taken together, our results demonstrate that the phenylpropanoid pathway plays an important role in BPH resistance response, and provide valuable targets for genetic improvement of BPH resistance in rice.

scholarly journals A single nucleotide polymorphism in an R2R3 MYB transcription factor gene triggers the male sterility in soybean ms6 (Ames1)

2021 ◽  
Author(s):  
Junping Yu ◽  
Guolong Zhao ◽  
Wei Li ◽  
Ying Zhang ◽  
Peng Wang ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Glycine Max ◽  
Male Sterility ◽  
Bulked Segregant Analysis ◽  
Soybean Protein ◽  
Anther Development ◽  
Hybrid Breeding ◽  
Myb Transcription Factor ◽  
Male Sterile ◽  
R2r3 Myb

Abstract Key message Identification and functional analysis of the male sterile gene MS6 in Glycine max. Abstract Soybean (Glycine max (L.) Merr.) is an important crop providing vegetable oil and protein. The male sterility-based hybrid breeding is a promising method for improving soybean yield to meet the globally growing demand. In this research, we identified a soybean genic male sterile locus, MS6, by combining the bulked segregant analysis sequencing method and the map-based cloning technology. MS6, highly expressed in anther, encodes an R2R3 MYB transcription factor (GmTDF1-1) that is homologous to Tapetal Development and Function 1, a key factor for anther development in Arabidopsis and rice. In male sterile ms6 (Ames1), the mutant allele contains a missense mutation, leading to the 76th leucine substituted by histidine in the DNA binding domain of GmTDF1-1. The expression of soybean MS6 under the control of the AtTDF1 promoter could rescue the male sterility of attdf1 but ms6 could not. Additionally, ms6 overexpression in wild-type Arabidopsis did not affect anther development. These results evidence that GmTDF1-1 is a functional TDF1 homolog and L76H disrupts its function. Notably, GmTDF1-1 shows 92% sequence identity with another soybean protein termed as GmTDF1-2, whose active expression also restored the fertility of attdf1. However, GmTDF1-2 is constitutively expressed at a very low level in soybean, and therefore, not able to compensate for the MS6 deficiency. Analysis of the TDF1-involved anther development regulatory pathway showed that expressions of the genes downstream of TDF1 are significantly suppressed in ms6, unveiling that GmTDF1-1 is a core transcription factor regulating soybean anther development.

scholarly journals Different Mechanisms Participate in the R-dependent Activity of the R2R3 MYB Transcription Factor C1

2004 ◽  
Vol 279 (46) ◽  
pp. 48205-48213 ◽  
Author(s):  
J. Marcela Hernandez ◽  
George F. Heine ◽  
Niloufer G. Irani ◽  
Antje Feller ◽  
Min-Gab Kim ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Myb Transcription Factor ◽  
Dependent Activity ◽  
R2r3 Myb
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