scholarly journals Early Embryo Exposure to Assisted Reproductive Manipulation Induced Subtle Changes in Liver Epigenetics with No Apparent Negative Health Consequences in Rabbit

2021 ◽  
Vol 22 (18) ◽  
pp. 9716
Author(s):  
Ximo García-Domínguez ◽  
Gianfranco Diretto ◽  
David S. Peñaranda ◽  
Sarah Frusciante ◽  
Victor García-Carpintero ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Lipid Metabolism ◽  
Rabbit Model ◽  
Early Embryo ◽  
Multiple Ovulation ◽  
Metabolic Outcomes ◽  
Embryonic Exposure ◽  
Long Term Effect ◽  
Epigenetic Events

Embryo manipulation is a requisite step in assisted reproductive technology (ART). Therefore, it is of great necessity to appraise the safety of ART and investigate the long-term effect, including lipid metabolism, on ART-conceived offspring. Augmenting our ART rabbit model to investigate lipid metabolic outcomes in offspring longitudinally, we detected variations in hepatic DNA methylation ART offspring in the F3 generation for embryonic exposure (multiple ovulation, vitrification and embryo transfer). Through adult liver metabolomics and proteomics, we identified changes mainly related to lipid metabolism (e.g., polyunsaturated fatty acids, steroids, steroid hormone). We also found that DNA methylation analysis was linked to changes in lipid metabolism and apoptosis genes. Nevertheless, these differences did not apparently alter the general health status. Thus, our findings suggest that ART is likely to be a player in embryo epigenetic events related to hepatic homeostasis alteration in adulthood.

scholarly journals Long-term effect of sodium hyaluronate (Hyalgan®) on osteoarthritis progression in a rabbit model

2003 ◽  
Vol 11 (9) ◽  
pp. 636-643 ◽  
Author(s):  
D Amiel ◽  
T Toyoguchi ◽  
K Kobayashi ◽  
K Bowden ◽  
M.E Amiel ◽  
...  
Keyword(s):  
Rabbit Model ◽  
Sodium Hyaluronate ◽  
Term Effect ◽  
Long Term Effect ◽  
Osteoarthritis Progression

scholarly journals Epigenetic selection and the DNA methylation signatures of adverse prenatal environments

2018 ◽  
Author(s):  
Elmar W Tobi ◽  
Joost van den Heuvel ◽  
Bas J. Zwaan ◽  
L.H. Lumey ◽  
Bastiaan T. Heijmans ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Early Embryo ◽  
Intervention Strategies ◽  
Epigenetic Reprogramming ◽  
Epigenetic Mechanisms ◽  
Physiological Changes ◽  
Prenatally Exposed ◽  
Offspring Health ◽  
Plastic Responses

AbstractMaternal adversity is associated with long-term physiological changes in offspring. These are believed to be mediated through epigenetic mechanisms, including DNA methylation (DNAm). Changes in DNAm are often interpreted as damage or as part of plastic responses of the embryo. We propose that selection on stochastic DNAm differences generated during epigenetic reprogramming after fertilization contributes to the effects of maternal adversity on DNAm. Using a mathematical model of epigenetic reprogramming in the early embryo we predict that this “epigenetic selection” will generate a characteristic reduction in variance of DNAm at selected loci in populations exposed to maternal adversity. We tested this prediction using DNAm data from a human cohort prenatally exposed to the Dutch Famine and confirmed the reduction in DNAm variance, suggesting that epigenetic selection may have occurred. Epigenetic selection should be considered as a possible mechanism linking adversity in pregnancy to offspring health and may have implications for the likely effectiveness of intervention strategies.

scholarly journals DNA Methylation Mediates the Long Term Effect of Breastfeeding Duration on Eczema at Age 18 Years

2018 ◽  
Vol 141 (2) ◽  
pp. AB187
Author(s):  
Nandini Mukherjee ◽  
Hasan Arshad ◽  
John W. Holloway ◽  
Hongmei Zhang ◽  
Wilfried Karmaus
Keyword(s):  
Dna Methylation ◽  
Term Effect ◽  
Breastfeeding Duration ◽  
Long Term Effect

scholarly journals An experimental, non-uremic rabbit model of peritoneal dialysis

2008 ◽  
pp. 253-260
Author(s):  
S Zunic-Bozinovski ◽  
Z Lausevic ◽  
S Krstic ◽  
N Jovanovic ◽  
J Trbojevic-Stankovic ◽  
...  
Keyword(s):  
Peritoneal Dialysis ◽  
Rabbit Model ◽  
Peritoneal Membrane ◽  
Experimental Procedure ◽  
Long Term Effect ◽  
Catheter Obstruction ◽  
Dialysis Solutions ◽  
Dialysis Fluids ◽  
Infusion System

Peritoneal dialysis (PD) is a well established method of depuration in uremic patients. Standard dialysis solutions currently in use are not biocompatible with the peritoneal membrane. Studying effects of dialysate on peritoneal membrane in humans is still a challenge. There is no consensus on the ideal experimental model so far. We, therefore, wanted to develop a new experimental non-uremic rabbit model of peritoneal dialysis, which would be practical, easy to conduct, not too costly, and convenient to investigate the long-term effect of dialysis fluids. The study was done on 17 healthy Chinchilla male and female rabbits, anesthetized with Thiopental in a dose of 0.5 mg/kg body mass. A catheter, specially made from Tro-soluset (Troge Medical GMBH, Hamburg, Germany) infusion system, was then surgically inserted and tunneled from animals' abdomen to their neck. The planned experimental procedure was 4 weeks of peritoneal dialysate instillation. The presented non-uremic rabbit model of peritoneal dialysis is relatively inexpensive, does not require sophisticated technology and was well tolerated by the animals. Complications such as peritonitis, dialysis fluid leakage, constipation and catheter obstruction were negligible. This model is reproducible and can be used to analyze the effects of different dialysis solutions on the rabbit peritoneal membrane.

Long-Term Effect of Bran Ingestion on Lipid Metabolism in Healthy Man

1986 ◽  
Vol 30 (6) ◽  
pp. 369-373 ◽  
Author(s):  
P. Gariot ◽  
J.P. Digy ◽  
P. Genton ◽  
D. Lambert ◽  
R.M.H. Bau ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
Term Effect ◽  
Healthy Man ◽  
Long Term Effect

scholarly journals COVID-19 Is a Multi-Organ Aggressor: Epigenetic and Clinical Marks

2021 ◽  
Vol 12 ◽  
Author(s):  
Mankgopo Magdeline Kgatle ◽  
Ismaheel Opeyemi Lawal ◽  
Gabriel Mashabela ◽  
Tebatso Moshoeu Gillian Boshomane ◽  
Palesa Caroline Koatale ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Environmental Factors ◽  
Severe Acute Respiratory Syndrome ◽  
Viral Replication ◽  
Clinical Significance ◽  
Clinical Manifestation ◽  
Epigenetic Events ◽  
Genetic And Environmental Factors ◽  
Control And Management

The progression of coronavirus disease 2019 (COVID-19), resulting from a severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, may be influenced by both genetic and environmental factors. Several viruses hijack the host genome machinery for their own advantage and survival, and similar phenomena might occur upon SARS-CoV-2 infection. Severe cases of COVID-19 may be driven by metabolic and epigenetic driven mechanisms, including DNA methylation and histone/chromatin alterations. These epigenetic phenomena may respond to enhanced viral replication and mediate persistent long-term infection and clinical phenotypes associated with severe COVID-19 cases and fatalities. Understanding the epigenetic events involved, and their clinical significance, may provide novel insights valuable for the therapeutic control and management of the COVID-19 pandemic. This review highlights different epigenetic marks potentially associated with COVID-19 development, clinical manifestation, and progression.

A long-term effect of subliminally presented words

2010 ◽  
Author(s):  
Andreas T. Breuer ◽  
Michael E. J. Masson ◽  
Glen E. Bodner
Keyword(s):  
Term Effect ◽  
Long Term Effect
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