Global Transcriptomic Analysis Reveals Differentially Expressed Genes Involved in Embryogenic Callus Induction in Drumstick (Moringa oleifera Lam.)

The plant embryogenic callus (EC) is an irregular embryogenic cell mass with strong regenerative ability that can be used for propagation and genetic transformation. However, difficulties with EC induction have hindered the breeding of drumstick, a tree with diverse potential commercial uses. In this study, three drumstick EC cDNA libraries were sequenced using an Illumina NovaSeq 6000 system. A total of 7191 differentially expressed genes (DEGs) for embryogenic callus development were identified, of which 2325 were mapped to the KEGG database, with the categories of plant hormone signal transduction and Plant-pathogen interaction being well-represented. The results obtained suggest that auxin and cytokinin metabolism and several embryogenesis-labeled genes are involved in embryogenic callus induction. Additionally, 589 transcription factors from 20 different families were differentially expressed during EC formation. The differential expression of 16 unigenes related to auxin signaling pathways was validated experimentally by quantitative real time PCR (qRT-PCR) using samples representing three sequential developmental stages of drumstick EC, supporting their apparent involvement in drumstick EC formation. Our study provides valuable information about the molecular mechanism of EC formation and has revealed new genes involved in this process.

Download Full-text

Suppression Subtractive Hybridization cDNA Libraries to Identify Differentially Expressed Genes from Contrasting Fish Habitats

Marine Biotechnology ◽

10.1007/s10126-004-3146-6 ◽

2004 ◽

Vol 6 (4) ◽

pp. 386-399 ◽

Cited By ~ 22

Author(s):

Peter F. Straub ◽

Mary L. Higham ◽

Arnaud Tanguy ◽

Brenda J. Landau ◽

William C. Phoel ◽

...

Keyword(s):

Differentially Expressed Genes ◽

Suppression Subtractive Hybridization ◽

Subtractive Hybridization ◽

Differentially Expressed ◽

Cdna Libraries ◽

Fish Habitats

Download Full-text

Embryogenic callus induction and fatty acid composition analysis of oil palm (Elaeis guineensis cv. Tenera)

Scientia Horticulturae ◽

10.1016/j.scienta.2018.10.014 ◽

2019 ◽

Vol 245 ◽

pp. 125-130 ◽

Cited By ~ 3

Author(s):

Jixin Zou ◽

Qing Zhang ◽

Zhiyong Zhu ◽

Lingchao Gao ◽

Yusheng Zheng ◽

...

Keyword(s):

Fatty Acid Composition ◽

Fatty Acid ◽

Acid Composition ◽

Oil Palm ◽

Callus Induction ◽

Embryogenic Callus ◽

Elaeis Guineensis ◽

Composition Analysis ◽

Embryogenic Callus Induction ◽

Fatty Acid Composition Analysis

Download Full-text

Embryogenic Callus Induction of Aquilaira Malaccensis Lam. and Aquilaria Subintegra Ding Hou

International Journal of Engineering and Advanced Technology - Regular Issue ◽

10.35940/ijeat.a3057.109119 ◽

2019 ◽

Vol 9 (1) ◽

pp. 5746-5751

Keyword(s):

Callus Induction ◽

Embryogenic Callus ◽

Callus Formation ◽

Dichlorophenoxyacetic Acid ◽

Aseptic Culture ◽

Aquilaria Malaccensis ◽

The Family ◽

Commercially Important ◽

Embryogenic Callus Induction ◽

2,4 Dichlorophenoxyacetic Acid

Aquilaria malaccensis Lam. and Aquilaria subintegra Ding Hou belong to the family of Thymelaeaceae which is commonly known as gaharu or agarwood. It is a commercially important tree and identified as a potential aromatic plant. The overwhelming responses in the lodging sector reduce gaharu species in the forest. Mass propagation through plant tissue culture technology will substitute this problem. The present study was conducted to investigate the embryogenic callus induction between these two species. The most optimum sterilization method for both species was sodium hypochlorite 5.0% which gave the highest percentage of aseptic culture (95%) with the absence of tissue browning. The leaves of both species were cultured on Murashige and Skoog, (1962) (MS) media supplemented with combination of various concentrations of 6-benzylaminopurine (BAP) (0.5, 1.0, 2.0 and 2.5 mg/L) and 2,4-dichlorophenoxyacetic acid (2, 4-D) (0.5, 1.0, 1.5 and 2.0 mg/L) and kept under dark condition. The explants produced embryogenic, white and compact callus at the end cut of the explants after two weeks of culture in all treatments. The highest frequency of embryogenic callus formation was observed in explants cultured on 2.0 mg/L BAP and 0.5 mg/L 2,4-D for both species. From the present study, the optimum sterilization technique and embryogenic callus induction for A. malaccensis Lam. and A. subintegra were established.

Download Full-text